{"id":{"repo_id":"umn","oai_identifier":"oai:conservancy.umn.edu:11299/278172"},"canonical_url":"https://search.dev.ndltd.org/etd/umn/oai:conservancy.umn.edu:11299/278172","repository":{"repo_id":"umn","name":"University of Minnesota","base_url":"https://conservancy.umn.edu/server/oai/request"},"display":{"title":"Expanding the synthetic cell toolkit: programmable fusion for complex genetic circuits and a synthetic cell cycle","abstract":"Synthetic biology as a scientific discipline is relatively new; however, major strides have been taken to develop current in vitro technologies into platforms that can imitate life. Currently, synthetic cell technologies often address only one feature of life (cell division, protein expression, DNA replication, etc.) due to the technological limitations of the current systems. In this dissertation, major improvements in the synthetic cell field are achieved by coupling well-established liposomal technologies with other novel technologies. The first of these is the development of a synthetic cell system that uses programmable liposomal fusion events to control and regulate complex genetic circuits originating from within synthetic cells. The second combines several synthetic cell technologies to realize a model cell cycle within these life-like liposomal bioreactors. Although the product of this dissertation is quite obviously not fully “alive,” major improvements in current methodologies were achieved, allowing for these artificially produced synthetic cells to blur the line a even more between in vitro systems and biological life.","abstract_html":"Synthetic biology as a scientific discipline is relatively new; however, major strides have been taken to develop current in vitro technologies into platforms that can imitate life. Currently, synthetic cell technologies often address only one feature of life (cell division, protein expression, DNA replication, etc.) due to the technological limitations of the current systems. In this dissertation, major improvements in the synthetic cell field are achieved by coupling well-established liposomal technologies with other novel technologies. The first of these is the development of a synthetic cell system that uses programmable liposomal fusion events to control and regulate complex genetic circuits originating from within synthetic cells. The second combines several synthetic cell technologies to realize a model cell cycle within these life-like liposomal bioreactors. Although the product of this dissertation is quite obviously not fully “alive,” major improvements in current methodologies were achieved, allowing for these artificially produced synthetic cells to blur the line a even more between in vitro systems and biological life.","abstract_has_math":false,"creators":["Gaut, Nathaniel"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022","date_published":"2022","updated_at":"2026-07-24T05:19:44Z","subjects":["Genetic Circuits","Liposomes","Synthetic Biology","Synthetic Cells"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/11299/278172","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Gaut, Nathaniel"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2026-02-03T19:58:12Z"]},{"key":"dc:date.issued","label":"Date","values":["2022"]},{"key":"dc:type","label":"Dc Type","values":["Thesis or Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Genetic Circuits","Liposomes","Synthetic Biology","Synthetic Cells"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/11299/278172"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["University of Minnesota Ph.D. dissertation. 2022. Major: Biological Science. Advisors: Katarzyna Adamala, Aaron Engelhart. 1 computer file (PDF); viii, 174 pages."]},{"key":"dc:description.abstract","label":"Abstract","values":["Synthetic biology as a scientific discipline is relatively new; however, major strides have been taken to develop current in vitro technologies into platforms that can imitate life. Currently, synthetic cell technologies often address only one feature of life (cell division, protein expression, DNA replication, etc.) due to the technological limitations of the current systems. In this dissertation, major improvements in the synthetic cell field are achieved by coupling well-established liposomal technologies with other novel technologies. The first of these is the development of a synthetic cell system that uses programmable liposomal fusion events to control and regulate complex genetic circuits originating from within synthetic cells. The second combines several synthetic cell technologies to realize a model cell cycle within these life-like liposomal bioreactors. Although the product of this dissertation is quite obviously not fully “alive,” major improvements in current methodologies were achieved, allowing for these artificially produced synthetic cells to blur the line a even more between in vitro systems and biological life."]},{"key":"dc:title","label":"Title","values":["Expanding the synthetic cell toolkit: programmable fusion for complex genetic circuits and a synthetic cell cycle"]}]}],"canonical_facts":{"dc:creator":["Gaut, Nathaniel"],"dc:date.accessioned":["2026-02-03T19:58:12Z"],"dc:date.issued":["2022"],"dc:description":["University of Minnesota Ph.D. dissertation. 2022. Major: Biological Science. Advisors: Katarzyna Adamala, Aaron Engelhart. 1 computer file (PDF); viii, 174 pages."],"dc:description.abstract":["Synthetic biology as a scientific discipline is relatively new; however, major strides have been taken to develop current in vitro technologies into platforms that can imitate life. Currently, synthetic cell technologies often address only one feature of life (cell division, protein expression, DNA replication, etc.) due to the technological limitations of the current systems. In this dissertation, major improvements in the synthetic cell field are achieved by coupling well-established liposomal technologies with other novel technologies. The first of these is the development of a synthetic cell system that uses programmable liposomal fusion events to control and regulate complex genetic circuits originating from within synthetic cells. The second combines several synthetic cell technologies to realize a model cell cycle within these life-like liposomal bioreactors. Although the product of this dissertation is quite obviously not fully “alive,” major improvements in current methodologies were achieved, allowing for these artificially produced synthetic cells to blur the line a even more between in vitro systems and biological life."],"dc:identifier.uri":["https://hdl.handle.net/11299/278172"],"dc:language.iso":["en"],"dc:subject":["Genetic Circuits","Liposomes","Synthetic Biology","Synthetic Cells"],"dc:title":["Expanding the synthetic cell toolkit: programmable fusion for complex genetic circuits and a synthetic cell cycle"],"dc:type":["Thesis or Dissertation"]},"updated_at":"2026-07-24T05:19:44Z"}