{"id":{"repo_id":"umn","oai_identifier":"oai:conservancy.umn.edu:11299/201096"},"canonical_url":"https://search.dev.ndltd.org/etd/umn/oai:conservancy.umn.edu:11299/201096","repository":{"repo_id":"umn","name":"University of Minnesota","base_url":"https://conservancy.umn.edu/server/oai/request"},"display":{"title":"Integrating Genomics and Metabolomics to Inform Breeding for Powdery Mildew Resistance in Grapevine","abstract":"Two powdery mildew resistance loci have been identified using pedigree-connected F1 mapping families at the University of Minnesota grape breeding program. A consensus linkage map of the resistant parent (MN1264) was developed for genetic mapping. The resistance loci were mapped on chromosomes 2 and 15, with additive effects accounting for over 30% phenotypic variation. Marker haplotypes, hap+chr2 and hap+chr15, were constructed to trace the inheritance of resistance loci in grandparent-parent-progeny relationships. Both hap+chr2 and hap+chr15 in the resistant F1 progeny were inherited from parent MN1264, that originated from grandparent ‘Seyval blanc’. Additionally, two microsatellites markers (i.e., UDV-015b and VViv67) were identified to be associated with hap+chr15, and can be applied for marker-assisted selection. In a follow-up study to characterize metabolic changes attributed to hap+chr2 and hap+chr15, a metabolomic experiment was conducted on whole-plant propagated grapes in a time-course response to in vivo inoculation. The use of several multivariate analyses systematically identified 52 biomarkers that were associated with hap+chr2, and 12 biomarkers with hap+chr15. In a temporal assessment of biomarkers, the discriminating metabolic changes distinguishing resistant and susceptible individuals appeared to be occurring from 24 to 48 hours after inoculation.","abstract_html":"Two powdery mildew resistance loci have been identified using pedigree-connected F1 mapping families at the University of Minnesota grape breeding program. A consensus linkage map of the resistant parent (MN1264) was developed for genetic mapping. The resistance loci were mapped on chromosomes 2 and 15, with additive effects accounting for over 30% phenotypic variation. Marker haplotypes, hap+chr2 and hap+chr15, were constructed to trace the inheritance of resistance loci in grandparent-parent-progeny relationships. Both hap+chr2 and hap+chr15 in the resistant F1 progeny were inherited from parent MN1264, that originated from grandparent ‘Seyval blanc’. Additionally, two microsatellites markers (i.e., UDV-015b and VViv67) were identified to be associated with hap+chr15, and can be applied for marker-assisted selection. In a follow-up study to characterize metabolic changes attributed to hap+chr2 and hap+chr15, a metabolomic experiment was conducted on whole-plant propagated grapes in a time-course response to in vivo inoculation. The use of several multivariate analyses systematically identified 52 biomarkers that were associated with hap+chr2, and 12 biomarkers with hap+chr15. In a temporal assessment of biomarkers, the discriminating metabolic changes distinguishing resistant and susceptible individuals appeared to be occurring from 24 to 48 hours after inoculation.","abstract_has_math":false,"creators":["Teh, Soon Li"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-08","date_published":"2018-08","updated_at":"2026-07-24T05:19:52Z","subjects":["breeding","genetics","genomics","grapevine","marker","metabolomics"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11299/201096","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Teh, Soon Li"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2018-11-28T14:11:56Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2018-11-28T14:11:56Z"]},{"key":"dc:date.issued","label":"Date","values":["2018-08"]},{"key":"dc:type","label":"Dc Type","values":["Thesis or Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["breeding","genetics","genomics","grapevine","marker","metabolomics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11299/201096"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["University of Minnesota Ph.D. dissertation. August 2018. Major: Applied Plant Sciences. Advisors: Adrian Hegeman, James Luby. 1 computer file (PDF); x, 135 pages."]},{"key":"dc:description.abstract","label":"Abstract","values":["Two powdery mildew resistance loci have been identified using pedigree-connected F1 mapping families at the University of Minnesota grape breeding program. A consensus linkage map of the resistant parent (MN1264) was developed for genetic mapping. The resistance loci were mapped on chromosomes 2 and 15, with additive effects accounting for over 30% phenotypic variation. Marker haplotypes, hap+chr2 and hap+chr15, were constructed to trace the inheritance of resistance loci in grandparent-parent-progeny relationships. Both hap+chr2 and hap+chr15 in the resistant F1 progeny were inherited from parent MN1264, that originated from grandparent ‘Seyval blanc’. Additionally, two microsatellites markers (i.e., UDV-015b and VViv67) were identified to be associated with hap+chr15, and can be applied for marker-assisted selection. In a follow-up study to characterize metabolic changes attributed to hap+chr2 and hap+chr15, a metabolomic experiment was conducted on whole-plant propagated grapes in a time-course response to in vivo inoculation. The use of several multivariate analyses systematically identified 52 biomarkers that were associated with hap+chr2, and 12 biomarkers with hap+chr15. In a temporal assessment of biomarkers, the discriminating metabolic changes distinguishing resistant and susceptible individuals appeared to be occurring from 24 to 48 hours after inoculation."]},{"key":"dc:title","label":"Title","values":["Integrating Genomics and Metabolomics to Inform Breeding for Powdery Mildew Resistance in Grapevine"]}]}],"canonical_facts":{"dc:creator":["Teh, Soon Li"],"dc:date.accessioned":["2018-11-28T14:11:56Z"],"dc:date.available":["2018-11-28T14:11:56Z"],"dc:date.issued":["2018-08"],"dc:description":["University of Minnesota Ph.D. dissertation. August 2018. Major: Applied Plant Sciences. Advisors: Adrian Hegeman, James Luby. 1 computer file (PDF); x, 135 pages."],"dc:description.abstract":["Two powdery mildew resistance loci have been identified using pedigree-connected F1 mapping families at the University of Minnesota grape breeding program. A consensus linkage map of the resistant parent (MN1264) was developed for genetic mapping. The resistance loci were mapped on chromosomes 2 and 15, with additive effects accounting for over 30% phenotypic variation. Marker haplotypes, hap+chr2 and hap+chr15, were constructed to trace the inheritance of resistance loci in grandparent-parent-progeny relationships. Both hap+chr2 and hap+chr15 in the resistant F1 progeny were inherited from parent MN1264, that originated from grandparent ‘Seyval blanc’. Additionally, two microsatellites markers (i.e., UDV-015b and VViv67) were identified to be associated with hap+chr15, and can be applied for marker-assisted selection. In a follow-up study to characterize metabolic changes attributed to hap+chr2 and hap+chr15, a metabolomic experiment was conducted on whole-plant propagated grapes in a time-course response to in vivo inoculation. The use of several multivariate analyses systematically identified 52 biomarkers that were associated with hap+chr2, and 12 biomarkers with hap+chr15. In a temporal assessment of biomarkers, the discriminating metabolic changes distinguishing resistant and susceptible individuals appeared to be occurring from 24 to 48 hours after inoculation."],"dc:identifier.uri":["http://hdl.handle.net/11299/201096"],"dc:language.iso":["en"],"dc:subject":["breeding","genetics","genomics","grapevine","marker","metabolomics"],"dc:title":["Integrating Genomics and Metabolomics to Inform Breeding for Powdery Mildew Resistance in Grapevine"],"dc:type":["Thesis or Dissertation"]},"updated_at":"2026-07-24T05:19:52Z"}