Development of Closomer And MIBG based Nanoformulation: Application to HIV Prevention and Neuroblastoma Therapy
type: CreativeWork
description: This dissertation is divided into two parts. Part #1 focuses on the development and characterization of closomer based nanoformulation for the prevention of HIV/AIDS sexual transmission. Part #2 focuses on the development and characterization of a meta-iodobenzylguadinine (MIBG) based nanoformulation of etoposide (VP-16) intended for the treatment of neuroblastoma. This work is presented in 6 chapters as described below, and partially in 3 peer-reviewed papers (see appendix). Part #1 and #2 are summarized in Chapters 4-5 and 6, respectively. In Chapters 1 and 2, an overview of the aims and scope of this study, and a literature review of the two parts of this dissertation is extensively discussed. In Chapter 3, it was hypothesized that fluorine 19 nuclear magnetic resonance (¹⁹F qNMR) can be used for real time quantification and in vitro release of maraviroc (MVC) – a first in its class FDA approved HIV entry inhibitor. The calibration assay produced a linearity curve in concentration range (0.42 mg/ml – 15 mg/ml), and the limits of detection and quantification values were 0.97 mg/ml and 2.93 mg/ml, respectively. The method was confirmed to be specific, accurate, precise, and robust (%RSE < 2%). The motivation for this study is that knowledge gained can be applied to subsequent work; quantifying, in real time fluorinated drug potency, purity, and stability, and in evaluating in vitro drug release kinetic from different formulations, in different biological medias. In chapter 4, it was hypothesized that phenyboronic acids (PBA) (gp-120 ligand) and MVC conjugated to closomer scaffold will be safer and more effective in HIV/AIDS prevention. The subsequent conjugation was completed via a one-pot microwave-assisted organic synthesis, and the reaction conditions were optimized using a screening method. Proton (¹H), carbon (¹³C), boron (¹¹B) NMR, and Fourier transform infrared (FTIR) spectroscopy were used to monitor the chemical reaction progress and elucidate the chemical…
abstract: This dissertation is divided into two parts. Part #1 focuses on the development and characterization of closomer based nanoformulation for the prevention of HIV/AIDS sexual transmission. Part #2 focuses on the development and characterization of a meta-iodobenzylguadinine (MIBG) based nanoformulation of etoposide (VP-16) intended for the treatment of neuroblastoma. This work is presented in 6 chapters as described below, and partially in 3 peer-reviewed papers (see appendix). Part #1 and #2 are summarized in Chapters 4-5 and 6, respectively. In Chapters 1 and 2, an overview of the aims and scope of this study, and a literature review of the two parts of this dissertation is extensively discussed. In Chapter 3, it was hypothesized that fluorine 19 nuclear magnetic resonance (¹⁹F qNMR) can be used for real time quantification and in vitro release of maraviroc (MVC) – a first in its class FDA approved HIV entry inhibitor. The calibration assay produced a linearity curve in concentration range (0.42 mg/ml – 15 mg/ml), and the limits of detection and quantification values were 0.97 mg/ml and 2.93 mg/ml, respectively. The method was confirmed to be specific, accurate, precise, and robust (%RSE < 2%). The motivation for this study is that knowledge gained can be applied to subsequent work; quantifying, in real time fluorinated drug potency, purity, and stability, and in evaluating in vitro drug release kinetic from different formulations, in different biological medias. In chapter 4, it was hypothesized that phenyboronic acids (PBA) (gp-120 ligand) and MVC conjugated to closomer scaffold will be safer and more effective in HIV/AIDS prevention. The subsequent conjugation was completed via a one-pot microwave-assisted organic synthesis, and the reaction conditions were optimized using a screening method. Proton (¹H), carbon (¹³C), boron (¹¹B) NMR, and Fourier transform infrared (FTIR) spectroscopy were used to monitor the chemical reaction progress and elucidate the chemical…
author: Akinjole, Omowumi Victoria
date published: 2023
date modified: 2026-07-24T05:19:15Z
provider: University of Missouri - Kansas City
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Missouri--Kansas City

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property ID: Handle
value: https://hdl.handle.net/10355/96477