{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/98165"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/98165","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Functional analysis of soybean dirigent genes","abstract":"In this project, the expression of the soybean dirigent genes and their effect on plant defense to pathogens was studied. Dirigent proteins, present in all of the major land plants, have been hypothesized to work in concert with oxidative enzymes to direct the synthesis of stereospecific lignans, such as (+) or (-)-pinoresinol, and lignins, from the selective coupling of specific stereoisomers of deprotonated alcohol molecules. Lignans and lignins have been shown to have antimicrobial properties and human health benefits when polymerized. The expression patterns in leaves of several genes encoding dirigent-like proteins in soybean suggest that these genes are pathogen-induced. The first goal of this research project was to identify all dirigent-like genes in soybean. Once identified, alignments and a phylogenetic tree of their protein sequences were made, and 3 selected ones were chemically synthesized and overexpressed in Arabidopsis thaliana to determine in the future if they function in these plants to enhance resistance to pathogens. In addition, I analyzed the promoter of the 3 selected dirigent genes that were determined to be the most pathogen-specific in its expression pattern. Transient transformation and disease assays were performed in Nicotiana benthamiana and soybean, showing possible reduction in the size of lesions induced by Sclerotinia sclerotiorum when the soybean dirigent-like genes were transiently expressed in N. benthamiana.","abstract_html":"In this project, the expression of the soybean dirigent genes and their effect on plant defense to pathogens was studied. Dirigent proteins, present in all of the major land plants, have been hypothesized to work in concert with oxidative enzymes to direct the synthesis of stereospecific lignans, such as (+) or (-)-pinoresinol, and lignins, from the selective coupling of specific stereoisomers of deprotonated alcohol molecules. Lignans and lignins have been shown to have antimicrobial properties and human health benefits when polymerized. The expression patterns in leaves of several genes encoding dirigent-like proteins in soybean suggest that these genes are pathogen-induced. The first goal of this research project was to identify all dirigent-like genes in soybean. Once identified, alignments and a phylogenetic tree of their protein sequences were made, and 3 selected ones were chemically synthesized and overexpressed in Arabidopsis thaliana to determine in the future if they function in these plants to enhance resistance to pathogens. In addition, I analyzed the promoter of the 3 selected dirigent genes that were determined to be the most pathogen-specific in its expression pattern. Transient transformation and disease assays were performed in Nicotiana benthamiana and soybean, showing possible reduction in the size of lesions induced by Sclerotinia sclerotiorum when the soybean dirigent-like genes were transiently expressed in N. benthamiana.","abstract_has_math":false,"creators":["Berruezo, Florencia"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Crop Sciences","degree_department":null,"school":null,"contributors":["Clough, Steven J.","Domier, Leslie","Lambert, Kris"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-09-29T17:45:35Z","date_published":"2017-09-29T17:45:35Z","updated_at":"2026-07-22T22:24:35Z","subjects":["Dirigents (DIR)","Lignin","Lignan","Soybean","Pathogen-induced defense"],"languages":["en"],"rights":["Copyright 2017 Florencia Berruezo"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/98165","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Clough, Steven J.","Domier, Leslie","Lambert, Kris"]},{"key":"dc:creator","label":"Author","values":["Berruezo, Florencia"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2017-09-29T17:45:35Z","2020-03-03T10:15:25Z","2017-07-17","2017-08"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Dirigents (DIR)","Lignin","Lignan","Soybean","Pathogen-induced defense"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2017 Florencia Berruezo"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/98165"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["In this project, the expression of the soybean dirigent genes and their effect on plant defense to pathogens was studied. Dirigent proteins, present in all of the major land plants, have been hypothesized to work in concert with oxidative enzymes to direct the synthesis of stereospecific lignans, such as (+) or (-)-pinoresinol, and lignins, from the selective coupling of specific stereoisomers of deprotonated alcohol molecules. Lignans and lignins have been shown to have antimicrobial properties and human health benefits when polymerized. The expression patterns in leaves of several genes encoding dirigent-like proteins in soybean suggest that these genes are pathogen-induced. The first goal of this research project was to identify all dirigent-like genes in soybean. Once identified, alignments and a phylogenetic tree of their protein sequences were made, and 3 selected ones were chemically synthesized and overexpressed in Arabidopsis thaliana to determine in the future if they function in these plants to enhance resistance to pathogens. In addition, I analyzed the promoter of the 3 selected dirigent genes that were determined to be the most pathogen-specific in its expression pattern. Transient transformation and disease assays were performed in Nicotiana benthamiana and soybean, showing possible reduction in the size of lesions induced by Sclerotinia sclerotiorum when the soybean dirigent-like genes were transiently expressed in N. benthamiana.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2019-08-01","The student, Florencia Berruezo, accepted the attached license on 2017-06-14 at 14:37.","The student, Florencia Berruezo, submitted this Thesis for approval on 2017-06-14 at 14:42.","This Thesis was approved for publication on 2017-07-17 at 08:57.","DSpace SAF Submission Ingestion Package generated from Vireo submission #11213 on 2017-09-29 at 10:45:47","Made available in DSpace on 2017-09-29T17:45:35Z (GMT). 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Dirigent proteins, present in all of the major land plants, have been hypothesized to work in concert with oxidative enzymes to direct the synthesis of stereospecific lignans, such as (+) or (-)-pinoresinol, and lignins, from the selective coupling of specific stereoisomers of deprotonated alcohol molecules. Lignans and lignins have been shown to have antimicrobial properties and human health benefits when polymerized. The expression patterns in leaves of several genes encoding dirigent-like proteins in soybean suggest that these genes are pathogen-induced. The first goal of this research project was to identify all dirigent-like genes in soybean. Once identified, alignments and a phylogenetic tree of their protein sequences were made, and 3 selected ones were chemically synthesized and overexpressed in Arabidopsis thaliana to determine in the future if they function in these plants to enhance resistance to pathogens. In addition, I analyzed the promoter of the 3 selected dirigent genes that were determined to be the most pathogen-specific in its expression pattern. Transient transformation and disease assays were performed in Nicotiana benthamiana and soybean, showing possible reduction in the size of lesions induced by Sclerotinia sclerotiorum when the soybean dirigent-like genes were transiently expressed in N. benthamiana.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2019-08-01","The student, Florencia Berruezo, accepted the attached license on 2017-06-14 at 14:37.","The student, Florencia Berruezo, submitted this Thesis for approval on 2017-06-14 at 14:42.","This Thesis was approved for publication on 2017-07-17 at 08:57.","DSpace SAF Submission Ingestion Package generated from Vireo submission #11213 on 2017-09-29 at 10:45:47","Made available in DSpace on 2017-09-29T17:45:35Z (GMT). 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