{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/98156"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/98156","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Noise in noise out - single molecule studies of molecular motors","abstract":"Many cellular functions rely on the movements of molecular motors. Single molecule studies of these motors allow investigation into their step-sizes and stall forces. Such studies inevitably involve noise, which is inherent in the biological and imaging systems probing these motors. Having to work with noise in the system, we develop a parameter, called the FIONA Index, which serves as a guide for data quality based on noise per step-size (NPS) and frames per step (FPS). Attempting to bring the noise out of the system, we develop Magnetic Cytoskeleton Affinity (MiCA) purification to remove excess dyes that prevent clear observation of individual motors. We show that with appropriate treatment of noise, complex systems can be assembled to study how multiple motors work together.","abstract_html":"Many cellular functions rely on the movements of molecular motors. Single molecule studies of these motors allow investigation into their step-sizes and stall forces. Such studies inevitably involve noise, which is inherent in the biological and imaging systems probing these motors. Having to work with noise in the system, we develop a parameter, called the FIONA Index, which serves as a guide for data quality based on noise per step-size (NPS) and frames per step (FPS). Attempting to bring the noise out of the system, we develop Magnetic Cytoskeleton Affinity (MiCA) purification to remove excess dyes that prevent clear observation of individual motors. We show that with appropriate treatment of noise, complex systems can be assembled to study how multiple motors work together.","abstract_has_math":false,"creators":["Tjioe, Marco"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biophysics & Computnl Biology","degree_department":null,"school":null,"contributors":["Selvin, Paul R.","Chemla, Yann R.","DeMarco, Brian L.","Zhang, Kai"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-09-29T17:45:38Z","date_published":"2017-09-29T17:45:38Z","updated_at":"2026-07-22T22:24:35Z","subjects":["Kinesin","Single molecule","Dynein","Noise","Frames per step (FPS)","Magnetic purification","Signal-to-noise ratio","Molecular motor"],"languages":["en"],"rights":["Copyright 2017 Marco Tjioe"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/98156","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Selvin, Paul R.","Chemla, Yann R.","DeMarco, Brian L.","Zhang, Kai"]},{"key":"dc:creator","label":"Author","values":["Tjioe, Marco"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2017-09-29T17:45:38Z","2020-03-03T10:15:38Z","2017-05-26","2017-08"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biophysics & Computnl Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Kinesin","Single molecule","Dynein","Noise","Frames per step (FPS)","Magnetic purification","Signal-to-noise ratio","Molecular motor"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2017 Marco Tjioe"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/98156"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Many cellular functions rely on the movements of molecular motors. Single molecule studies of these motors allow investigation into their step-sizes and stall forces. Such studies inevitably involve noise, which is inherent in the biological and imaging systems probing these motors. Having to work with noise in the system, we develop a parameter, called the FIONA Index, which serves as a guide for data quality based on noise per step-size (NPS) and frames per step (FPS). Attempting to bring the noise out of the system, we develop Magnetic Cytoskeleton Affinity (MiCA) purification to remove excess dyes that prevent clear observation of individual motors. We show that with appropriate treatment of noise, complex systems can be assembled to study how multiple motors work together.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2019-08-01","The student, Marco Tjioe, accepted the attached license on 2017-05-22 at 16:46.","The student, Marco Tjioe, submitted this Dissertation for approval on 2017-05-22 at 16:53.","This Dissertation was approved for publication on 2017-05-26 at 11:59.","DSpace SAF Submission Ingestion Package generated from Vireo submission #11170 on 2017-09-29 at 10:45:35","Made available in DSpace on 2017-09-29T17:45:38Z (GMT). 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Single molecule studies of these motors allow investigation into their step-sizes and stall forces. Such studies inevitably involve noise, which is inherent in the biological and imaging systems probing these motors. Having to work with noise in the system, we develop a parameter, called the FIONA Index, which serves as a guide for data quality based on noise per step-size (NPS) and frames per step (FPS). Attempting to bring the noise out of the system, we develop Magnetic Cytoskeleton Affinity (MiCA) purification to remove excess dyes that prevent clear observation of individual motors. We show that with appropriate treatment of noise, complex systems can be assembled to study how multiple motors work together.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2019-08-01","The student, Marco Tjioe, accepted the attached license on 2017-05-22 at 16:46.","The student, Marco Tjioe, submitted this Dissertation for approval on 2017-05-22 at 16:53.","This Dissertation was approved for publication on 2017-05-26 at 11:59.","DSpace SAF Submission Ingestion Package generated from Vireo submission #11170 on 2017-09-29 at 10:45:35","Made available in DSpace on 2017-09-29T17:45:38Z (GMT). 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