Characterization of the vaccinia virus ankyrin repeat protein K1 on NF-κB inhibition and its contributions to viral pathogenesis
type: CreativeWork
description: All viruses strategically alter the anti-viral immune response to their benefit. The goal of this thesis was to examine how the vaccinia virus (VACV) K1 protein, which is encoded by the K1L gene, modulates the immune response in vitro and in vivo. In vitro, the K1 protein has multiple immunomodulatory effects in tissue culture models of infection. We have previously published that the K1 protein inhibits double-stranded RNA-dependent protein kinase (PKR) activation. A consequence of this function is that K1 inhibits PKR-induced NF-κB activation during VACV infection. However, transient expression of K1 also inhibits Toll-like receptor (TLR)-induced NF-κB activation. This suggested that K1 had a second NF-κB inhibitory mechanism that is PKR-independent. This possibility was explored by expressing K1 independently of infection and stimulating NF-κB under conditions that minimized or excluded PKR activation. Using these approaches, I found a second NF-κB inhibitory function for K1: K1 inhibited nuclear acetylation of the RelA (p65) subunit of NF-κB. Moreover, p65-CBP interactions were blocked in the presence of K1. However, K1 did not preclude NF-κB binding to oligonucleotides containing B binding sites. This implies that NF-κB-promoter interactions still occurred in the presence of K1, but NF-κB cannot properly trigger transcriptional activation because K1 antagonizes acetylation of RelA. These data show that K1 has multiple mechanisms to inhibit NF-κB activation and, in comparison to all known VACV NF-κB inhibitory proteins, K1 acts at one of the most downstream events of NF-κB activation. A second goal of my thesis was to understand the contribution of K1 in vivo. I determined that a K1L-less vaccinia virus (vΔK1L) was less pathogenic than wild-type VACV in intranasal and intradermal models of infection. Decreased pathogenicity correlated with diminished virus replication in intranasally-infected mice. However, in intradermally-inoculated ears, vΔK1L replicated to n…
abstract: All viruses strategically alter the anti-viral immune response to their benefit. The goal of this thesis was to examine how the vaccinia virus (VACV) K1 protein, which is encoded by the K1L gene, modulates the immune response in vitro and in vivo. In vitro, the K1 protein has multiple immunomodulatory effects in tissue culture models of infection. We have previously published that the K1 protein inhibits double-stranded RNA-dependent protein kinase (PKR) activation. A consequence of this function is that K1 inhibits PKR-induced NF-κB activation during VACV infection. However, transient expression of K1 also inhibits Toll-like receptor (TLR)-induced NF-κB activation. This suggested that K1 had a second NF-κB inhibitory mechanism that is PKR-independent. This possibility was explored by expressing K1 independently of infection and stimulating NF-κB under conditions that minimized or excluded PKR activation. Using these approaches, I found a second NF-κB inhibitory function for K1: K1 inhibited nuclear acetylation of the RelA (p65) subunit of NF-κB. Moreover, p65-CBP interactions were blocked in the presence of K1. However, K1 did not preclude NF-κB binding to oligonucleotides containing B binding sites. This implies that NF-κB-promoter interactions still occurred in the presence of K1, but NF-κB cannot properly trigger transcriptional activation because K1 antagonizes acetylation of RelA. These data show that K1 has multiple mechanisms to inhibit NF-κB activation and, in comparison to all known VACV NF-κB inhibitory proteins, K1 acts at one of the most downstream events of NF-κB activation. A second goal of my thesis was to understand the contribution of K1 in vivo. I determined that a K1L-less vaccinia virus (vΔK1L) was less pathogenic than wild-type VACV in intranasal and intradermal models of infection. Decreased pathogenicity correlated with diminished virus replication in intranasally-infected mice. However, in intradermally-inoculated ears, vΔK1L replicated to n…
author: Bravo, Ariana G
language: en
keywords: Vaccinia, Poxvirus, K1, Inflammation, NF-κB
date published: 2017-08-10T20:33:05Z
date modified: 2026-07-22T22:24:34Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/97731