{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/97619"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/97619","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Engineered biomaterials as extracellular microenvironments for guiding cell programming and reprogramming","abstract":"The interface between cells and materials is a dynamic and complex environment where cells in contact with materials can sense their properties such as stiffness, matrix protein, and geometry and respond to these cues in multiple ways including through mechanical forces exerted on the matrix by the cells. Cells incorporate these cues via signal propagation through integrins, and translate this information through intracellular signal transduction cascades to regulate gene expression and cell fate decisions. Advances in biomaterials to direct stem cell lineage decisions have focused on designing biomimetic materials that realize the ‘‘in vivo” microenvironments’ ability to interact with cells. However, not only is designing tailored biomaterials that present multiple signals challenging, but the precise roles of physical and biochemical cues in coordinating cellular processes such as migration, proliferation, and differentiation remains difficult to dissect. After a short introduction we explore using model polyacrylamide hydrogel systems in Chapter 2-5 to study the effects of biophysical (elasticity and geometry) and chemical (matrix protein) cues on mesenchymal stem cell (MSC) fate decisions, showing these cues can play a large role in differentiation. In Chapter 6 we explore how switching the biophysical microenvironment (matrix stiffness and cell shape) can be used to understand the plasticity of MSC lineage specification. Finally, in Chapter 7-9, we demonstrate how geometric cues at the interface of tissue, where interfacial energy and curvature can be modulated in vitro, will dictate cancer cell tumorigenicity, metastatic potential, and the regulation of tumorangiogenesis. Moreover, we reveal a mechanism where perimeter features initiate α5β1 adhesion and epithelial-to-mesenchymal transition, Mitogen Activated Protein Kinase (MAPK) and Signal Transducer and Activator of Transcription (STAT) pathways, and regulation of distinct histone marks, to guide gene expression underlying the phenotypic alterations of malignant melanoma. Overall, we believe the work presented here demonstrates the importance and utility of extracellular properties in modulating cell programming and reprogramming, and should aid in the development of biomaterials for more efficiently directing distinct cellular states for the development of synthetic model systems that more accurately recapitulate the in vivo microenvironment.","abstract_html":"The interface between cells and materials is a dynamic and complex environment where cells in contact with materials can sense their properties such as stiffness, matrix protein, and geometry and respond to these cues in multiple ways including through mechanical forces exerted on the matrix by the cells. Cells incorporate these cues via signal propagation through integrins, and translate this information through intracellular signal transduction cascades to regulate gene expression and cell fate decisions. Advances in biomaterials to direct stem cell lineage decisions have focused on designing biomimetic materials that realize the ‘‘in vivo” microenvironments’ ability to interact with cells. However, not only is designing tailored biomaterials that present multiple signals challenging, but the precise roles of physical and biochemical cues in coordinating cellular processes such as migration, proliferation, and differentiation remains difficult to dissect. After a short introduction we explore using model polyacrylamide hydrogel systems in Chapter 2-5 to study the effects of biophysical (elasticity and geometry) and chemical (matrix protein) cues on mesenchymal stem cell (MSC) fate decisions, showing these cues can play a large role in differentiation. In Chapter 6 we explore how switching the biophysical microenvironment (matrix stiffness and cell shape) can be used to understand the plasticity of MSC lineage specification. Finally, in Chapter 7-9, we demonstrate how geometric cues at the interface of tissue, where interfacial energy and curvature can be modulated in vitro, will dictate cancer cell tumorigenicity, metastatic potential, and the regulation of tumorangiogenesis. Moreover, we reveal a mechanism where perimeter features initiate α5β1 adhesion and epithelial-to-mesenchymal transition, Mitogen Activated Protein Kinase (MAPK) and Signal Transducer and Activator of Transcription (STAT) pathways, and regulation of distinct histone marks, to guide gene expression underlying the phenotypic alterations of malignant melanoma. Overall, we believe the work presented here demonstrates the importance and utility of extracellular properties in modulating cell programming and reprogramming, and should aid in the development of biomaterials for more efficiently directing distinct cellular states for the development of synthetic model systems that more accurately recapitulate the in vivo microenvironment.","abstract_has_math":false,"creators":["Lee, Junmin"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Materials Science & Engr","degree_department":null,"school":null,"contributors":["Kilian, Kristopher A.","Cheng, Jianjun","Fan, Timothy M.","Leal, Cecilia"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-08-10T19:52:20Z","date_published":"2017-08-10T19:52:20Z","updated_at":"2026-07-22T22:24:34Z","subjects":["Biomaterials","Extracellular microenvironment","Stiffness","Matrix protein","Geometry"],"languages":["en"],"rights":["Copyright 2017 Junmin Lee"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/97619","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kilian, Kristopher A.","Cheng, Jianjun","Fan, Timothy M.","Leal, Cecilia"]},{"key":"dc:creator","label":"Author","values":["Lee, Junmin"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2017-08-10T19:52:20Z","2019-08-11T09:15:24Z","2017-04-21","2017-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Materials Science & Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biomaterials","Extracellular microenvironment","Stiffness","Matrix protein","Geometry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2017 Junmin Lee"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/97619"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The interface between cells and materials is a dynamic and complex environment where cells in contact with materials can sense their properties such as stiffness, matrix protein, and geometry and respond to these cues in multiple ways including through mechanical forces exerted on the matrix by the cells. Cells incorporate these cues via signal propagation through integrins, and translate this information through intracellular signal transduction cascades to regulate gene expression and cell fate decisions. Advances in biomaterials to direct stem cell lineage decisions have focused on designing biomimetic materials that realize the ‘‘in vivo” microenvironments’ ability to interact with cells. However, not only is designing tailored biomaterials that present multiple signals challenging, but the precise roles of physical and biochemical cues in coordinating cellular processes such as migration, proliferation, and differentiation remains difficult to dissect. After a short introduction we explore using model polyacrylamide hydrogel systems in Chapter 2-5 to study the effects of biophysical (elasticity and geometry) and chemical (matrix protein) cues on mesenchymal stem cell (MSC) fate decisions, showing these cues can play a large role in differentiation. In Chapter 6 we explore how switching the biophysical microenvironment (matrix stiffness and cell shape) can be used to understand the plasticity of MSC lineage specification. Finally, in Chapter 7-9, we demonstrate how geometric cues at the interface of tissue, where interfacial energy and curvature can be modulated in vitro, will dictate cancer cell tumorigenicity, metastatic potential, and the regulation of tumorangiogenesis. Moreover, we reveal a mechanism where perimeter features initiate α5β1 adhesion and epithelial-to-mesenchymal transition, Mitogen Activated Protein Kinase (MAPK) and Signal Transducer and Activator of Transcription (STAT) pathways, and regulation of distinct histone marks, to guide gene expression underlying the phenotypic alterations of malignant melanoma. Overall, we believe the work presented here demonstrates the importance and utility of extracellular properties in modulating cell programming and reprogramming, and should aid in the development of biomaterials for more efficiently directing distinct cellular states for the development of synthetic model systems that more accurately recapitulate the in vivo microenvironment.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2019-05-01","The student, Junmin Lee, accepted the attached license on 2017-04-21 at 11:46.","The student, Junmin Lee, submitted this Dissertation for approval on 2017-04-21 at 11:58.","This Dissertation was approved for publication on 2017-04-21 at 13:50.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10975 on 2017-08-10 at 14:32:27","Made available in DSpace on 2017-08-10T19:52:20Z (GMT). No. of bitstreams: 3 LEE-DISSERTATION-2017.pdf: 12737884 bytes, checksum: 7a816a91105b15add05afb41e8474e1d (MD5) LICENSE.txt: 4207 bytes, checksum: 7039eb18ea9eb981f8e66fde919f9e33 (MD5) PROQUEST_LICENSE.txt: 4553 bytes, checksum: 67fe70c299caf7701aad02094b2ea05b (MD5) Previous issue date: 2017-04-21","Embargo set by: Colleen Fallaw for item 102672 Lift date: 2019-08-10T21:25:30Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 102672 on 2019-08-11T09:15:24Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Engineered biomaterials as extracellular microenvironments for guiding cell programming and reprogramming"]}]}],"canonical_facts":{"dc:contributor":["Kilian, Kristopher A.","Cheng, Jianjun","Fan, Timothy M.","Leal, Cecilia"],"dc:creator":["Lee, Junmin"],"dc:date":["2017-08-10T19:52:20Z","2019-08-11T09:15:24Z","2017-04-21","2017-05"],"dc:description":["The interface between cells and materials is a dynamic and complex environment where cells in contact with materials can sense their properties such as stiffness, matrix protein, and geometry and respond to these cues in multiple ways including through mechanical forces exerted on the matrix by the cells. Cells incorporate these cues via signal propagation through integrins, and translate this information through intracellular signal transduction cascades to regulate gene expression and cell fate decisions. Advances in biomaterials to direct stem cell lineage decisions have focused on designing biomimetic materials that realize the ‘‘in vivo” microenvironments’ ability to interact with cells. However, not only is designing tailored biomaterials that present multiple signals challenging, but the precise roles of physical and biochemical cues in coordinating cellular processes such as migration, proliferation, and differentiation remains difficult to dissect. After a short introduction we explore using model polyacrylamide hydrogel systems in Chapter 2-5 to study the effects of biophysical (elasticity and geometry) and chemical (matrix protein) cues on mesenchymal stem cell (MSC) fate decisions, showing these cues can play a large role in differentiation. In Chapter 6 we explore how switching the biophysical microenvironment (matrix stiffness and cell shape) can be used to understand the plasticity of MSC lineage specification. Finally, in Chapter 7-9, we demonstrate how geometric cues at the interface of tissue, where interfacial energy and curvature can be modulated in vitro, will dictate cancer cell tumorigenicity, metastatic potential, and the regulation of tumorangiogenesis. Moreover, we reveal a mechanism where perimeter features initiate α5β1 adhesion and epithelial-to-mesenchymal transition, Mitogen Activated Protein Kinase (MAPK) and Signal Transducer and Activator of Transcription (STAT) pathways, and regulation of distinct histone marks, to guide gene expression underlying the phenotypic alterations of malignant melanoma. Overall, we believe the work presented here demonstrates the importance and utility of extracellular properties in modulating cell programming and reprogramming, and should aid in the development of biomaterials for more efficiently directing distinct cellular states for the development of synthetic model systems that more accurately recapitulate the in vivo microenvironment.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2019-05-01","The student, Junmin Lee, accepted the attached license on 2017-04-21 at 11:46.","The student, Junmin Lee, submitted this Dissertation for approval on 2017-04-21 at 11:58.","This Dissertation was approved for publication on 2017-04-21 at 13:50.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10975 on 2017-08-10 at 14:32:27","Made available in DSpace on 2017-08-10T19:52:20Z (GMT). No. of bitstreams: 3 LEE-DISSERTATION-2017.pdf: 12737884 bytes, checksum: 7a816a91105b15add05afb41e8474e1d (MD5) LICENSE.txt: 4207 bytes, checksum: 7039eb18ea9eb981f8e66fde919f9e33 (MD5) PROQUEST_LICENSE.txt: 4553 bytes, checksum: 67fe70c299caf7701aad02094b2ea05b (MD5) Previous issue date: 2017-04-21","Embargo set by: Colleen Fallaw for item 102672 Lift date: 2019-08-10T21:25:30Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 102672 on 2019-08-11T09:15:24Z."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/97619"],"dc:language":["en"],"dc:rights":["Copyright 2017 Junmin Lee"],"dc:subject":["Biomaterials","Extracellular microenvironment","Stiffness","Matrix protein","Geometry"],"dc:title":["Engineered biomaterials as extracellular microenvironments for guiding cell programming and reprogramming"],"dc:type":["text"],"thesis:degree_discipline":["Materials Science & Engr"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:34Z"}