{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/97563"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/97563","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Discovery and analysis of long noncoding RNAs in gene expression control and cell cycle progression","abstract":"Recent advances in transcriptome analysis have revealed that a large proportion of the mammalian genome is transcribed. Thousands of these transcripts are classified as long non-coding RNAs (lncRNAs), with size larger than 200 nucleotides, but very few have been functionally characterized. Here, I detail a journey of the discovery and analysis of lncRNAs in gene expression control and cell cycle progression, beginning from the characterization of a mutual regulation between lncRNA MALAT1 and its natural antisense transcript TALAM1, followed by the identification and functional characterization of lncRNAs involved in cell cycle progression. The mutual regulation between MALAT1 and TALAM1 presents a novel feed-forward positive regulatory loop at the MALAT1 locus where MALAT1 positively regulates the transcription and stability of TALAM1, and TALAM1 in turn promotes the processing and maturation events of MALAT1 which are essential to maintain the high cellular levels of MALAT1. In the characterization of functional lncRNAs in cell cycle progression, I focus on an S phase-upregulated lncRNA, termed S7, and demonstrate that it plays crucial roles in cell cycle progression and tumorigenicity, through regulating the expression of genes in the cellular proliferation network including the Hippo signaling pathway. Altogether, these studies support a model whereby pervasive lncRNA transcripts, previously regarded as transcriptional byproducts, function through diverse mechanisms as critical regulators of gene expression and the vital cell cycle processes.","abstract_html":"Recent advances in transcriptome analysis have revealed that a large proportion of the mammalian genome is transcribed. Thousands of these transcripts are classified as long non-coding RNAs (lncRNAs), with size larger than 200 nucleotides, but very few have been functionally characterized. Here, I detail a journey of the discovery and analysis of lncRNAs in gene expression control and cell cycle progression, beginning from the characterization of a mutual regulation between lncRNA MALAT1 and its natural antisense transcript TALAM1, followed by the identification and functional characterization of lncRNAs involved in cell cycle progression. The mutual regulation between MALAT1 and TALAM1 presents a novel feed-forward positive regulatory loop at the MALAT1 locus where MALAT1 positively regulates the transcription and stability of TALAM1, and TALAM1 in turn promotes the processing and maturation events of MALAT1 which are essential to maintain the high cellular levels of MALAT1. In the characterization of functional lncRNAs in cell cycle progression, I focus on an S phase-upregulated lncRNA, termed S7, and demonstrate that it plays crucial roles in cell cycle progression and tumorigenicity, through regulating the expression of genes in the cellular proliferation network including the Hippo signaling pathway. Altogether, these studies support a model whereby pervasive lncRNA transcripts, previously regarded as transcriptional byproducts, function through diverse mechanisms as critical regulators of gene expression and the vital cell cycle processes.","abstract_has_math":false,"creators":["Zong, Xinying"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Cell and Developmental Biology","degree_department":null,"school":null,"contributors":["Prasanth, K.V.","Ceman, Stephanie S.","Chen, Jie","Schuler, Mary A.","Katzenellenbogen, Benita S."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-08-10T19:51:52Z","date_published":"2017-08-10T19:51:52Z","updated_at":"2026-07-22T22:24:34Z","subjects":["Long noncoding RNA","Metastasis associated lung adenocarcinoma transcript 1 (MALAT1)","Cell cycle","Natural antisense transcript"],"languages":["en"],"rights":["Copyright 2017 Xinying Zong"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/97563","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Prasanth, K.V.","Ceman, Stephanie S.","Chen, Jie","Schuler, Mary A.","Katzenellenbogen, Benita S."]},{"key":"dc:creator","label":"Author","values":["Zong, Xinying"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2017-08-10T19:51:52Z","2021-02-17T10:15:42Z","2017-04-14","2017-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Cell and Developmental Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Long noncoding RNA","Metastasis associated lung adenocarcinoma transcript 1 (MALAT1)","Cell cycle","Natural antisense transcript"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2017 Xinying Zong"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/97563"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Recent advances in transcriptome analysis have revealed that a large proportion of the mammalian genome is transcribed. Thousands of these transcripts are classified as long non-coding RNAs (lncRNAs), with size larger than 200 nucleotides, but very few have been functionally characterized. Here, I detail a journey of the discovery and analysis of lncRNAs in gene expression control and cell cycle progression, beginning from the characterization of a mutual regulation between lncRNA MALAT1 and its natural antisense transcript TALAM1, followed by the identification and functional characterization of lncRNAs involved in cell cycle progression. The mutual regulation between MALAT1 and TALAM1 presents a novel feed-forward positive regulatory loop at the MALAT1 locus where MALAT1 positively regulates the transcription and stability of TALAM1, and TALAM1 in turn promotes the processing and maturation events of MALAT1 which are essential to maintain the high cellular levels of MALAT1. In the characterization of functional lncRNAs in cell cycle progression, I focus on an S phase-upregulated lncRNA, termed S7, and demonstrate that it plays crucial roles in cell cycle progression and tumorigenicity, through regulating the expression of genes in the cellular proliferation network including the Hippo signaling pathway. Altogether, these studies support a model whereby pervasive lncRNA transcripts, previously regarded as transcriptional byproducts, function through diverse mechanisms as critical regulators of gene expression and the vital cell cycle processes.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2019-05-01","The student, Xinying Zong, accepted the attached license on 2017-04-12 at 23:30.","The student, Xinying Zong, submitted this Dissertation for approval on 2017-04-12 at 23:42.","This Dissertation was approved for publication on 2017-04-14 at 14:27.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10734 on 2017-08-10 at 14:30:27","Made available in DSpace on 2017-08-10T19:51:52Z (GMT). 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Thousands of these transcripts are classified as long non-coding RNAs (lncRNAs), with size larger than 200 nucleotides, but very few have been functionally characterized. Here, I detail a journey of the discovery and analysis of lncRNAs in gene expression control and cell cycle progression, beginning from the characterization of a mutual regulation between lncRNA MALAT1 and its natural antisense transcript TALAM1, followed by the identification and functional characterization of lncRNAs involved in cell cycle progression. The mutual regulation between MALAT1 and TALAM1 presents a novel feed-forward positive regulatory loop at the MALAT1 locus where MALAT1 positively regulates the transcription and stability of TALAM1, and TALAM1 in turn promotes the processing and maturation events of MALAT1 which are essential to maintain the high cellular levels of MALAT1. In the characterization of functional lncRNAs in cell cycle progression, I focus on an S phase-upregulated lncRNA, termed S7, and demonstrate that it plays crucial roles in cell cycle progression and tumorigenicity, through regulating the expression of genes in the cellular proliferation network including the Hippo signaling pathway. Altogether, these studies support a model whereby pervasive lncRNA transcripts, previously regarded as transcriptional byproducts, function through diverse mechanisms as critical regulators of gene expression and the vital cell cycle processes.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2019-05-01","The student, Xinying Zong, accepted the attached license on 2017-04-12 at 23:30.","The student, Xinying Zong, submitted this Dissertation for approval on 2017-04-12 at 23:42.","This Dissertation was approved for publication on 2017-04-14 at 14:27.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10734 on 2017-08-10 at 14:30:27","Made available in DSpace on 2017-08-10T19:51:52Z (GMT). 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