{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/97537"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/97537","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Discovery and structural characterization of lanthipeptides from soil and rumen bacteria","abstract":"As members of the ribosomally synthesized and post-translationally modified peptide natural products (RiPPs), lanthipeptides possess myriad structural diversity and potential for discovery using genome-guided approaches. Biological activities of interest embedded within lanthipeptide structures include antimicrobial, antiviral, and morphogenetic functions. Continued discovery efforts seek to expand the characterized activities and structures of these compounds, as well as the enzymes that execute their biosynthesis. The contents of this thesis are focused on the discovery of lanthipeptides using a variety of methods. Chapters 2 and 4 demonstrate the application of heterologous expression of gene clusters to produce lanthipeptides. The resulting peptide structures and enzyme activities provide intriguing insights into diversity of biosynthetic approaches present in this class of compounds. Furthermore, the abundance of precursor peptides encoded in the lanthipeptide biosynthetic systems discussed in Chapters 2 and 4 offer examples of combinatorial biosynthesis and the tantalizing possibility to harness this attribute. In contrast, Chapter 3 provides a glimpse into the targeted screening of Actinobacteria for the production of lanthipeptides, which suggests additional structural diversity. The successful application of these methods increases the number of lanthipeptide compounds and systems, which ultimately allows for a deeper understanding of and appreciation for this class of natural products.","abstract_html":"As members of the ribosomally synthesized and post-translationally modified peptide natural products (RiPPs), lanthipeptides possess myriad structural diversity and potential for discovery using genome-guided approaches. Biological activities of interest embedded within lanthipeptide structures include antimicrobial, antiviral, and morphogenetic functions. Continued discovery efforts seek to expand the characterized activities and structures of these compounds, as well as the enzymes that execute their biosynthesis. The contents of this thesis are focused on the discovery of lanthipeptides using a variety of methods. Chapters 2 and 4 demonstrate the application of heterologous expression of gene clusters to produce lanthipeptides. The resulting peptide structures and enzyme activities provide intriguing insights into diversity of biosynthetic approaches present in this class of compounds. Furthermore, the abundance of precursor peptides encoded in the lanthipeptide biosynthetic systems discussed in Chapters 2 and 4 offer examples of combinatorial biosynthesis and the tantalizing possibility to harness this attribute. In contrast, Chapter 3 provides a glimpse into the targeted screening of Actinobacteria for the production of lanthipeptides, which suggests additional structural diversity. The successful application of these methods increases the number of lanthipeptide compounds and systems, which ultimately allows for a deeper understanding of and appreciation for this class of natural products.","abstract_has_math":false,"creators":["Zhao, Xi Ling"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["van der Donk, Wilfred A.","Metcalf, William W.","Mitchell, Douglas A.","Sweedler, Jonathan V."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-08-10T19:51:37Z","date_published":"2017-08-10T19:51:37Z","updated_at":"2026-07-22T22:24:34Z","subjects":["Ribosomally synthesized and post-translationally modified peptide natural products (RiPPs)","Lanthipeptides","Heterologous expression of gene clusters","Combinatorial biosynthesis"],"languages":["en"],"rights":["Copyright 2017 Xi Zhao"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/97537","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["van der Donk, Wilfred A.","Metcalf, William W.","Mitchell, Douglas A.","Sweedler, Jonathan V."]},{"key":"dc:creator","label":"Author","values":["Zhao, Xi Ling"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2017-08-10T19:51:37Z","2019-08-11T09:15:31Z","2017-02-17","2017-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Ribosomally synthesized and post-translationally modified peptide natural products (RiPPs)","Lanthipeptides","Heterologous expression of gene clusters","Combinatorial biosynthesis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2017 Xi Zhao"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/97537"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["As members of the ribosomally synthesized and post-translationally modified peptide natural products (RiPPs), lanthipeptides possess myriad structural diversity and potential for discovery using genome-guided approaches. Biological activities of interest embedded within lanthipeptide structures include antimicrobial, antiviral, and morphogenetic functions. Continued discovery efforts seek to expand the characterized activities and structures of these compounds, as well as the enzymes that execute their biosynthesis. The contents of this thesis are focused on the discovery of lanthipeptides using a variety of methods. Chapters 2 and 4 demonstrate the application of heterologous expression of gene clusters to produce lanthipeptides. The resulting peptide structures and enzyme activities provide intriguing insights into diversity of biosynthetic approaches present in this class of compounds. Furthermore, the abundance of precursor peptides encoded in the lanthipeptide biosynthetic systems discussed in Chapters 2 and 4 offer examples of combinatorial biosynthesis and the tantalizing possibility to harness this attribute. In contrast, Chapter 3 provides a glimpse into the targeted screening of Actinobacteria for the production of lanthipeptides, which suggests additional structural diversity. The successful application of these methods increases the number of lanthipeptide compounds and systems, which ultimately allows for a deeper understanding of and appreciation for this class of natural products.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2019-05-01","The student, Xi Zhao, accepted the attached license on 2017-02-14 at 19:34.","The student, Xi Zhao, submitted this Dissertation for approval on 2017-02-14 at 19:41.","This Dissertation was approved for publication on 2017-02-17 at 09:12.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10562 on 2017-08-10 at 14:29:31","Made available in DSpace on 2017-08-10T19:51:37Z (GMT). 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Biological activities of interest embedded within lanthipeptide structures include antimicrobial, antiviral, and morphogenetic functions. Continued discovery efforts seek to expand the characterized activities and structures of these compounds, as well as the enzymes that execute their biosynthesis. The contents of this thesis are focused on the discovery of lanthipeptides using a variety of methods. Chapters 2 and 4 demonstrate the application of heterologous expression of gene clusters to produce lanthipeptides. The resulting peptide structures and enzyme activities provide intriguing insights into diversity of biosynthetic approaches present in this class of compounds. Furthermore, the abundance of precursor peptides encoded in the lanthipeptide biosynthetic systems discussed in Chapters 2 and 4 offer examples of combinatorial biosynthesis and the tantalizing possibility to harness this attribute. In contrast, Chapter 3 provides a glimpse into the targeted screening of Actinobacteria for the production of lanthipeptides, which suggests additional structural diversity. The successful application of these methods increases the number of lanthipeptide compounds and systems, which ultimately allows for a deeper understanding of and appreciation for this class of natural products.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2019-05-01","The student, Xi Zhao, accepted the attached license on 2017-02-14 at 19:34.","The student, Xi Zhao, submitted this Dissertation for approval on 2017-02-14 at 19:41.","This Dissertation was approved for publication on 2017-02-17 at 09:12.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10562 on 2017-08-10 at 14:29:31","Made available in DSpace on 2017-08-10T19:51:37Z (GMT). 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