{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/97342"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/97342","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"A bright, sweet evolution: a molecular genetic analysis of Calvin cycle gene expression response to light and sugar in the liverwort marchantia polymorpha, and insights into Calvin cycle promoter motif enrichment in land plants","abstract":"Higher plants are frequently used as model organisms in Calvin Benson Bassham cycle (CBB) research given their ease of use in the laboratory. The expression of CBB genes in higher plants is well known to be regulated by light, sugar and plant development, yet no focus has been given to lower plant CBB gene expression regulation. Marchantia polymorpha, a bryophyte in the Marchantiphyta division and newly emerging model liverwort plant, has been used in this study to demonstrate its potential use in CBB promoter evolution research. Transferring plants to darkness caused the downregulation of CBB genes MpSBPase, MpFBPase, MpPRK, MpRbcS1A and MpRbcS1B in Marchantia coinciding with the downregulation of these genes in higher plants. Marchantia CBB gene expression response to the exogenous sugars sucrose and glucose was also weakly downregulated, similar to higher plant CBB gene downregulation. However, a CBB promoter motif analysis between Marchantia and a higher plant Arabidopsis thaliana showed a major difference in over-represented motifs with an affinity to bind transcription factors responsive to light, sugar and development in MpCBB promoters compared to AtCBB promoters. Given that Marchantia is one of the earliest land plants, the findings here suggest that good model to understand the evolution of control of photosynthesis-related gene expression in the evolution of highly differentiated flowering plants.","abstract_html":"Higher plants are frequently used as model organisms in Calvin Benson Bassham cycle (CBB) research given their ease of use in the laboratory. The expression of CBB genes in higher plants is well known to be regulated by light, sugar and plant development, yet no focus has been given to lower plant CBB gene expression regulation. Marchantia polymorpha, a bryophyte in the Marchantiphyta division and newly emerging model liverwort plant, has been used in this study to demonstrate its potential use in CBB promoter evolution research. Transferring plants to darkness caused the downregulation of CBB genes MpSBPase, MpFBPase, MpPRK, MpRbcS1A and MpRbcS1B in Marchantia coinciding with the downregulation of these genes in higher plants. Marchantia CBB gene expression response to the exogenous sugars sucrose and glucose was also weakly downregulated, similar to higher plant CBB gene downregulation. However, a CBB promoter motif analysis between Marchantia and a higher plant Arabidopsis thaliana showed a major difference in over-represented motifs with an affinity to bind transcription factors responsive to light, sugar and development in MpCBB promoters compared to AtCBB promoters. Given that Marchantia is one of the earliest land plants, the findings here suggest that good model to understand the evolution of control of photosynthesis-related gene expression in the evolution of highly differentiated flowering plants.","abstract_has_math":false,"creators":["Massenburg, Lynnicia Naomi"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Plant Biology","degree_department":null,"school":null,"contributors":["Long, Steve","Ort, Don","Moose, Steve"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-08-10T19:14:57Z","date_published":"2017-08-10T19:14:57Z","updated_at":"2026-07-22T22:24:32Z","subjects":["Marchantia polymorpha","Liverwort","Arabdipsis thaliana","Dark response","Sugar response","Gene expression","Calvin cycle","Promoter motifs","Position weight matrix analysis"],"languages":["en"],"rights":["Copyright 2017 Lynnicia Massenburg"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/97342","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Long, Steve","Ort, Don","Moose, Steve"]},{"key":"dc:creator","label":"Author","values":["Massenburg, Lynnicia Naomi"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2017-08-10T19:14:57Z","2017-04-25","2017-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Plant Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Marchantia polymorpha","Liverwort","Arabdipsis thaliana","Dark response","Sugar response","Gene expression","Calvin cycle","Promoter motifs","Position weight matrix analysis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2017 Lynnicia Massenburg"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/97342"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Higher plants are frequently used as model organisms in Calvin Benson Bassham cycle (CBB) research given their ease of use in the laboratory. The expression of CBB genes in higher plants is well known to be regulated by light, sugar and plant development, yet no focus has been given to lower plant CBB gene expression regulation. Marchantia polymorpha, a bryophyte in the Marchantiphyta division and newly emerging model liverwort plant, has been used in this study to demonstrate its potential use in CBB promoter evolution research. Transferring plants to darkness caused the downregulation of CBB genes MpSBPase, MpFBPase, MpPRK, MpRbcS1A and MpRbcS1B in Marchantia coinciding with the downregulation of these genes in higher plants. Marchantia CBB gene expression response to the exogenous sugars sucrose and glucose was also weakly downregulated, similar to higher plant CBB gene downregulation. However, a CBB promoter motif analysis between Marchantia and a higher plant Arabidopsis thaliana showed a major difference in over-represented motifs with an affinity to bind transcription factors responsive to light, sugar and development in MpCBB promoters compared to AtCBB promoters. Given that Marchantia is one of the earliest land plants, the findings here suggest that good model to understand the evolution of control of photosynthesis-related gene expression in the evolution of highly differentiated flowering plants.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2017-08-10 without embargo terms","The student, Lynnicia Massenburg, accepted the attached license on 2017-04-21 at 14:42.","The student, Lynnicia Massenburg, submitted this Thesis for approval on 2017-04-21 at 14:55.","This Thesis was approved for publication on 2017-04-25 at 12:20.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10727 on 2017-08-10 at 13:39:33","Made available in DSpace on 2017-08-10T19:14:57Z (GMT). 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The expression of CBB genes in higher plants is well known to be regulated by light, sugar and plant development, yet no focus has been given to lower plant CBB gene expression regulation. Marchantia polymorpha, a bryophyte in the Marchantiphyta division and newly emerging model liverwort plant, has been used in this study to demonstrate its potential use in CBB promoter evolution research. Transferring plants to darkness caused the downregulation of CBB genes MpSBPase, MpFBPase, MpPRK, MpRbcS1A and MpRbcS1B in Marchantia coinciding with the downregulation of these genes in higher plants. Marchantia CBB gene expression response to the exogenous sugars sucrose and glucose was also weakly downregulated, similar to higher plant CBB gene downregulation. However, a CBB promoter motif analysis between Marchantia and a higher plant Arabidopsis thaliana showed a major difference in over-represented motifs with an affinity to bind transcription factors responsive to light, sugar and development in MpCBB promoters compared to AtCBB promoters. Given that Marchantia is one of the earliest land plants, the findings here suggest that good model to understand the evolution of control of photosynthesis-related gene expression in the evolution of highly differentiated flowering plants.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2017-08-10 without embargo terms","The student, Lynnicia Massenburg, accepted the attached license on 2017-04-21 at 14:42.","The student, Lynnicia Massenburg, submitted this Thesis for approval on 2017-04-21 at 14:55.","This Thesis was approved for publication on 2017-04-25 at 12:20.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10727 on 2017-08-10 at 13:39:33","Made available in DSpace on 2017-08-10T19:14:57Z (GMT). No. of bitstreams: 2 MASSENBURG-THESIS-2017.pdf: 3499664 bytes, checksum: 913ddff19d882ebfd008525b57bfa84b (MD5) LICENSE.txt: 4216 bytes, checksum: 2cc6154da1af850c556b0f48baf0019e (MD5) Previous issue date: 2017-04-25"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/97342"],"dc:language":["en"],"dc:rights":["Copyright 2017 Lynnicia Massenburg"],"dc:subject":["Marchantia polymorpha","Liverwort","Arabdipsis thaliana","Dark response","Sugar response","Gene expression","Calvin cycle","Promoter motifs","Position weight matrix analysis"],"dc:title":["A bright, sweet evolution: a molecular genetic analysis of Calvin cycle gene expression response to light and sugar in the liverwort marchantia polymorpha, and insights into Calvin cycle promoter motif enrichment in land plants"],"dc:type":["text"],"thesis:degree_discipline":["Plant Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:32Z"}