{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/97319"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/97319","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Pulmonary disposition and pharmacokinetics of minocycline in the adult horse","abstract":"The purpose of this study was to determine the pharmacokinetics and pulmonary disposition of minocycline in horses after a single intravenous (IV) and intragastric (IG) dose and after multiple IG doses. The study hypotheses were that: minocycline would be present in the pulmonary epithelial lining fluid (PELF) and bronchoalveolar lavage (BAL) cells at concentrations exceeding those in plasma within 3 hours of IV or IG administration and achievable trough concentrations in the PELF and BAL cells after administration of IG minocycline would exceed a target concentration of 0.25 µg/mL. Seven healthy adult horses from the resident teaching herd were used for the two part study. For part one of the study, 6 horses received IV (2.2 mg/kg) or IG (4 mg/kg) minocycline in a randomized cross-over design. Plasma samples were collected prior to minocycline administration and 16 times within 36 hours. Bronchoalveolar lavages were performed 4 times within 24 hours for collection of PELF and BAL cells. For part two of the study, minocycline (4 mg/kg) was administered IG every 12 hours for 5 doses to 6 horses. Plasma samples were collected before minocycline administration and 20 times within 96 hours. Bronchoalveolar lavages were performed 6 times within 72 hours for collection of PELF samples and BAL cells. In study 1, mean bioavailability of minocycline was calculated at 48% (range 35- 75%). In study 2, at steady state, mean + SD maximum concentration (Cmax) of minocycline in the plasma was 2.3 + 1.3 µg/mL and the terminal half-life was 11.8 + 0.5 hours. The median (25th and 75th percentiles) time to peak concentration (Tmax) was 1.3 (1.0 – 1.5) hours. The Cmax and Tmax of minocycline in the PELF were 10.5 + 12.8 µg/mL and 9.0 (5.5 – 12.0) hours, respectively. The Cmax and Tmax for BAL cells were 0.24 + 0.1 µg/mL and 6.0 (0.0 – 6.0) hours, respectively. Oral bioavailability (48%) varied considerably among adult horses (35 – 75%). While minocycline was detected in the PELF and BAL cells within 3 hours of IV or IG drug administration, only concentrations in the PELF exceeded those in plasma. As predicted, PELF trough concentrations exceeded the target concentration of 0.25 µg/mL at all measured time points. Contrary to the study hypothesis, minocycline BAL cell concentrations at all measured time points were well below concentrations detected in plasma and PELF and the 0.25 µg/mL target concentration. It was concluded that minocycline distributes into the PELF and BAL cells of adult horses.","abstract_html":"The purpose of this study was to determine the pharmacokinetics and pulmonary disposition of minocycline in horses after a single intravenous (IV) and intragastric (IG) dose and after multiple IG doses. The study hypotheses were that: minocycline would be present in the pulmonary epithelial lining fluid (PELF) and bronchoalveolar lavage (BAL) cells at concentrations exceeding those in plasma within 3 hours of IV or IG administration and achievable trough concentrations in the PELF and BAL cells after administration of IG minocycline would exceed a target concentration of 0.25 µg/mL. Seven healthy adult horses from the resident teaching herd were used for the two part study. For part one of the study, 6 horses received IV (2.2 mg/kg) or IG (4 mg/kg) minocycline in a randomized cross-over design. Plasma samples were collected prior to minocycline administration and 16 times within 36 hours. Bronchoalveolar lavages were performed 4 times within 24 hours for collection of PELF and BAL cells. For part two of the study, minocycline (4 mg/kg) was administered IG every 12 hours for 5 doses to 6 horses. Plasma samples were collected before minocycline administration and 20 times within 96 hours. Bronchoalveolar lavages were performed 6 times within 72 hours for collection of PELF samples and BAL cells. In study 1, mean bioavailability of minocycline was calculated at 48% (range 35- 75%). In study 2, at steady state, mean + SD maximum concentration (Cmax) of minocycline in the plasma was 2.3 + 1.3 µg/mL and the terminal half-life was 11.8 + 0.5 hours. The median (25th and 75th percentiles) time to peak concentration (Tmax) was 1.3 (1.0 – 1.5) hours. The Cmax and Tmax of minocycline in the PELF were 10.5 + 12.8 µg/mL and 9.0 (5.5 – 12.0) hours, respectively. The Cmax and Tmax for BAL cells were 0.24 + 0.1 µg/mL and 6.0 (0.0 – 6.0) hours, respectively. Oral bioavailability (48%) varied considerably among adult horses (35 – 75%). While minocycline was detected in the PELF and BAL cells within 3 hours of IV or IG drug administration, only concentrations in the PELF exceeded those in plasma. As predicted, PELF trough concentrations exceeded the target concentration of 0.25 µg/mL at all measured time points. Contrary to the study hypothesis, minocycline BAL cell concentrations at all measured time points were well below concentrations detected in plasma and PELF and the 0.25 µg/mL target concentration. It was concluded that minocycline distributes into the PELF and BAL cells of adult horses.","abstract_has_math":false,"creators":["Echeverria, Kate O"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"VMS-Veterinary Clinical Medcne","degree_department":null,"school":null,"contributors":["Lascola, Kara","Foreman, Jonathan","Austin, Scott"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-08-10T19:14:49Z","date_published":"2017-08-10T19:14:49Z","updated_at":"2026-07-22T22:24:32Z","subjects":["Pulmonary pharmacokinetics","Equine","Minocycline","Horse pneumonia","Pulmonary disposition","Pulmonary epithelial lining fluid","Bronchoalveolar lavage"],"languages":["en"],"rights":["Copyright 2017 Kate Echeverria"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/97319","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Lascola, Kara","Foreman, Jonathan","Austin, Scott"]},{"key":"dc:creator","label":"Author","values":["Echeverria, Kate O"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2017-08-10T19:14:49Z","2017-04-24","2017-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["VMS-Veterinary Clinical Medcne"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Pulmonary pharmacokinetics","Equine","Minocycline","Horse pneumonia","Pulmonary disposition","Pulmonary epithelial lining fluid","Bronchoalveolar lavage"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2017 Kate Echeverria"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/97319"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The purpose of this study was to determine the pharmacokinetics and pulmonary disposition of minocycline in horses after a single intravenous (IV) and intragastric (IG) dose and after multiple IG doses. The study hypotheses were that: minocycline would be present in the pulmonary epithelial lining fluid (PELF) and bronchoalveolar lavage (BAL) cells at concentrations exceeding those in plasma within 3 hours of IV or IG administration and achievable trough concentrations in the PELF and BAL cells after administration of IG minocycline would exceed a target concentration of 0.25 µg/mL. Seven healthy adult horses from the resident teaching herd were used for the two part study. For part one of the study, 6 horses received IV (2.2 mg/kg) or IG (4 mg/kg) minocycline in a randomized cross-over design. Plasma samples were collected prior to minocycline administration and 16 times within 36 hours. Bronchoalveolar lavages were performed 4 times within 24 hours for collection of PELF and BAL cells. For part two of the study, minocycline (4 mg/kg) was administered IG every 12 hours for 5 doses to 6 horses. Plasma samples were collected before minocycline administration and 20 times within 96 hours. Bronchoalveolar lavages were performed 6 times within 72 hours for collection of PELF samples and BAL cells. In study 1, mean bioavailability of minocycline was calculated at 48% (range 35- 75%). In study 2, at steady state, mean + SD maximum concentration (Cmax) of minocycline in the plasma was 2.3 + 1.3 µg/mL and the terminal half-life was 11.8 + 0.5 hours. The median (25th and 75th percentiles) time to peak concentration (Tmax) was 1.3 (1.0 – 1.5) hours. The Cmax and Tmax of minocycline in the PELF were 10.5 + 12.8 µg/mL and 9.0 (5.5 – 12.0) hours, respectively. The Cmax and Tmax for BAL cells were 0.24 + 0.1 µg/mL and 6.0 (0.0 – 6.0) hours, respectively. Oral bioavailability (48%) varied considerably among adult horses (35 – 75%). While minocycline was detected in the PELF and BAL cells within 3 hours of IV or IG drug administration, only concentrations in the PELF exceeded those in plasma. As predicted, PELF trough concentrations exceeded the target concentration of 0.25 µg/mL at all measured time points. Contrary to the study hypothesis, minocycline BAL cell concentrations at all measured time points were well below concentrations detected in plasma and PELF and the 0.25 µg/mL target concentration. It was concluded that minocycline distributes into the PELF and BAL cells of adult horses.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2017-08-10 without embargo terms","The student, Kate Echeverria, accepted the attached license on 2017-04-21 at 10:08.","The student, Kate Echeverria, submitted this Thesis for approval on 2017-04-21 at 10:08.","This Thesis was approved for publication on 2017-04-24 at 17:08.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10679 on 2017-08-10 at 13:39:02","Made available in DSpace on 2017-08-10T19:14:49Z (GMT). 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The study hypotheses were that: minocycline would be present in the pulmonary epithelial lining fluid (PELF) and bronchoalveolar lavage (BAL) cells at concentrations exceeding those in plasma within 3 hours of IV or IG administration and achievable trough concentrations in the PELF and BAL cells after administration of IG minocycline would exceed a target concentration of 0.25 µg/mL. Seven healthy adult horses from the resident teaching herd were used for the two part study. For part one of the study, 6 horses received IV (2.2 mg/kg) or IG (4 mg/kg) minocycline in a randomized cross-over design. Plasma samples were collected prior to minocycline administration and 16 times within 36 hours. Bronchoalveolar lavages were performed 4 times within 24 hours for collection of PELF and BAL cells. For part two of the study, minocycline (4 mg/kg) was administered IG every 12 hours for 5 doses to 6 horses. Plasma samples were collected before minocycline administration and 20 times within 96 hours. Bronchoalveolar lavages were performed 6 times within 72 hours for collection of PELF samples and BAL cells. In study 1, mean bioavailability of minocycline was calculated at 48% (range 35- 75%). In study 2, at steady state, mean + SD maximum concentration (Cmax) of minocycline in the plasma was 2.3 + 1.3 µg/mL and the terminal half-life was 11.8 + 0.5 hours. The median (25th and 75th percentiles) time to peak concentration (Tmax) was 1.3 (1.0 – 1.5) hours. The Cmax and Tmax of minocycline in the PELF were 10.5 + 12.8 µg/mL and 9.0 (5.5 – 12.0) hours, respectively. The Cmax and Tmax for BAL cells were 0.24 + 0.1 µg/mL and 6.0 (0.0 – 6.0) hours, respectively. Oral bioavailability (48%) varied considerably among adult horses (35 – 75%). While minocycline was detected in the PELF and BAL cells within 3 hours of IV or IG drug administration, only concentrations in the PELF exceeded those in plasma. As predicted, PELF trough concentrations exceeded the target concentration of 0.25 µg/mL at all measured time points. Contrary to the study hypothesis, minocycline BAL cell concentrations at all measured time points were well below concentrations detected in plasma and PELF and the 0.25 µg/mL target concentration. It was concluded that minocycline distributes into the PELF and BAL cells of adult horses.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2017-08-10 without embargo terms","The student, Kate Echeverria, accepted the attached license on 2017-04-21 at 10:08.","The student, Kate Echeverria, submitted this Thesis for approval on 2017-04-21 at 10:08.","This Thesis was approved for publication on 2017-04-24 at 17:08.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10679 on 2017-08-10 at 13:39:02","Made available in DSpace on 2017-08-10T19:14:49Z (GMT). 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