{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/95626"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/95626","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Xylan degradation in filamentous fungi and bacteria","abstract":"Embargo set by: Seth Robbins for item 98742 Lift date: 2019-03-01T17:05:02Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","abstract_html":"Embargo set by: Seth Robbins for item 98742 Lift date: 2019-03-01T17:05:02Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","abstract_has_math":false,"creators":["Vasconcelos Pereira, Gabriel"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Animal Sciences","degree_department":null,"school":null,"contributors":["Cann, Isaac","Mackie, Roderick","Ridlon, Jason"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-03-01T17:02:12Z","date_published":"2017-03-01T17:02:12Z","updated_at":"2026-07-22T22:26:37Z","subjects":["Xylan degradation","Filamentous fungi","Heterologous expression","Biofuels","Bacteria","Bacteroides","Human gut","Fiber degradation"],"languages":["en"],"rights":["Copyright 2016 Gabriel V. Pereira"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/95626","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Cann, Isaac","Mackie, Roderick","Ridlon, Jason"]},{"key":"dc:creator","label":"Author","values":["Vasconcelos Pereira, Gabriel"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2017-03-01T17:02:12Z","2019-03-02T10:15:30Z","2016-12-09","2016-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Xylan degradation","Filamentous fungi","Heterologous expression","Biofuels","Bacteria","Bacteroides","Human gut","Fiber degradation"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2016 Gabriel V. Pereira"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/95626"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Embargo set by: Seth Robbins for item 98742 Lift date: 2019-03-01T17:05:02Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Biochemical characterization of two recombinant gh10 family glycosyl hidrolases and differential expression in two model filamentous fungi Polysaccharides from plant cell walls are the most abundant biomass on Earth and are important resource for biofuel production. The main components of plant cell walls are cellulose, hemicellulose and lignin. Hemicellulose is the second most abundant component of renewable biomass and as arabinoxylan, is mainly composed of xylose and arabinose. Filamentous fungi, such as Trichoderma reesei, produce gram-per-liter levels of glycoside hydrolases (GHs). GH enzymes are required for hydrolysis of the glycosidic bonds present in complex polysaccharides, releasing fermentable sugars. In this project, we investigated the biochemical characteristics of two endoxylanases in two model filamentous fungi, Neurospora crassa and Aspergillus nidulans. Putative endoxylanase genes from N. crassa (ncu05924) and A. nidulans (an1818) were expressed homologously and heterologously in both filamentous fungi. Here, we demonstrate that A. nidulans was able to expressed and secrete at the same levels, both the recombinant homologous (AN1818) and heterologous (NCU05924) proteins, while N. crassa expressed the recombinant homologous protein at 26-fold more than the recombinant heterologous protein. All 4 endoxylanases had similar optimal pH (~5.8) and temperature (50 to ~55°C), similar secondary structures, and comparable glycosylation patterns. High performance liquid chromatography (HPLC) was used to identify the end products released by each enzyme from xylan substrates. The specific activity of AN1818 was ~50% higher than NCU05924 on different model xylans. Xylan degrading enzymes from human colonic bacteroides intestinalis1 Many human diets contain arabinoxylan, and the ease of genome sequencing coupled with reduced cost have led to unraveling the arsenal of genes utilized by the colonic Bacteroidetes to depolymerize this polysaccharide. The colonic Bacteroidetes with potential to ferment arabinoxylans include Bacteroides intestinalis. In this study, we analyzed the hydrolytic activities of members of a xylan degradation cluster encoded on the genome of Bacteroides intestinalis DSM 17393. Here, it is demonstrated that a cocktail of the xylanolytic enzymes completely hydrolyze arabinoxylans found in human diets. Fascinatingly, this bacterium and other relatives have evolved and secrete a unique bifunctional endoxylanase/arabinofuranosidase in the same polypeptide. The bifunctional enzyme and other secreted enzymes attack the polysaccharides extracellularly to remove the side-chains, exposing the xylan backbone for cleavage to xylo-oligosaccharides and xylose. These end products are transported into the cell where a β-xylosidase cleaves the oligosaccharides to fermentable sugars. While our experiments focused on B. intestinalis, it is likely that the extracellular enzymes also release nutrients to members of the colonic microbial community that practice cross-feeding. The conservation of the genes characterized in this study in other colonic Bacteroidetes alludes to a conserved strategy for energy acquisition from xylans, a component of human diets.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2018-12-01","The student, Gabriel Vasconcelos Pereira, accepted the attached license on 2016-12-09 at 10:19.","The student, Gabriel Vasconcelos Pereira, submitted this Thesis for approval on 2016-12-09 at 15:15.","This Thesis was approved for publication on 2016-12-09 at 16:58.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10491 on 2017-02-28 at 14:43:37","Made available in DSpace on 2017-03-01T17:02:12Z (GMT). No. of bitstreams: 2 VASCONCELOSPEREIRA-THESIS-2016.pdf: 3404837 bytes, checksum: 6f660473b69a7fcb74a63542419adafb (MD5) LICENSE.txt: 4224 bytes, checksum: 08adbaa72186ac97044b8588a6f48600 (MD5) Previous issue date: 2016-12-09","Embargo set by: Seth Robbins for item 98742 Lift date: 2019-03-01T17:02:22Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 98742 Lift date: 2019-03-01T17:03:32Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 98742 Lift date: 2019-03-01T17:06:55Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 98742 on 2019-03-02T10:15:30Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Xylan degradation in filamentous fungi and bacteria"]}]}],"canonical_facts":{"dc:contributor":["Cann, Isaac","Mackie, Roderick","Ridlon, Jason"],"dc:creator":["Vasconcelos Pereira, Gabriel"],"dc:date":["2017-03-01T17:02:12Z","2019-03-02T10:15:30Z","2016-12-09","2016-12"],"dc:description":["Embargo set by: Seth Robbins for item 98742 Lift date: 2019-03-01T17:05:02Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Biochemical characterization of two recombinant gh10 family glycosyl hidrolases and differential expression in two model filamentous fungi Polysaccharides from plant cell walls are the most abundant biomass on Earth and are important resource for biofuel production. The main components of plant cell walls are cellulose, hemicellulose and lignin. Hemicellulose is the second most abundant component of renewable biomass and as arabinoxylan, is mainly composed of xylose and arabinose. Filamentous fungi, such as Trichoderma reesei, produce gram-per-liter levels of glycoside hydrolases (GHs). GH enzymes are required for hydrolysis of the glycosidic bonds present in complex polysaccharides, releasing fermentable sugars. In this project, we investigated the biochemical characteristics of two endoxylanases in two model filamentous fungi, Neurospora crassa and Aspergillus nidulans. Putative endoxylanase genes from N. crassa (ncu05924) and A. nidulans (an1818) were expressed homologously and heterologously in both filamentous fungi. Here, we demonstrate that A. nidulans was able to expressed and secrete at the same levels, both the recombinant homologous (AN1818) and heterologous (NCU05924) proteins, while N. crassa expressed the recombinant homologous protein at 26-fold more than the recombinant heterologous protein. All 4 endoxylanases had similar optimal pH (~5.8) and temperature (50 to ~55°C), similar secondary structures, and comparable glycosylation patterns. High performance liquid chromatography (HPLC) was used to identify the end products released by each enzyme from xylan substrates. The specific activity of AN1818 was ~50% higher than NCU05924 on different model xylans. Xylan degrading enzymes from human colonic bacteroides intestinalis1 Many human diets contain arabinoxylan, and the ease of genome sequencing coupled with reduced cost have led to unraveling the arsenal of genes utilized by the colonic Bacteroidetes to depolymerize this polysaccharide. The colonic Bacteroidetes with potential to ferment arabinoxylans include Bacteroides intestinalis. In this study, we analyzed the hydrolytic activities of members of a xylan degradation cluster encoded on the genome of Bacteroides intestinalis DSM 17393. Here, it is demonstrated that a cocktail of the xylanolytic enzymes completely hydrolyze arabinoxylans found in human diets. Fascinatingly, this bacterium and other relatives have evolved and secrete a unique bifunctional endoxylanase/arabinofuranosidase in the same polypeptide. The bifunctional enzyme and other secreted enzymes attack the polysaccharides extracellularly to remove the side-chains, exposing the xylan backbone for cleavage to xylo-oligosaccharides and xylose. These end products are transported into the cell where a β-xylosidase cleaves the oligosaccharides to fermentable sugars. While our experiments focused on B. intestinalis, it is likely that the extracellular enzymes also release nutrients to members of the colonic microbial community that practice cross-feeding. The conservation of the genes characterized in this study in other colonic Bacteroidetes alludes to a conserved strategy for energy acquisition from xylans, a component of human diets.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2018-12-01","The student, Gabriel Vasconcelos Pereira, accepted the attached license on 2016-12-09 at 10:19.","The student, Gabriel Vasconcelos Pereira, submitted this Thesis for approval on 2016-12-09 at 15:15.","This Thesis was approved for publication on 2016-12-09 at 16:58.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10491 on 2017-02-28 at 14:43:37","Made available in DSpace on 2017-03-01T17:02:12Z (GMT). No. of bitstreams: 2 VASCONCELOSPEREIRA-THESIS-2016.pdf: 3404837 bytes, checksum: 6f660473b69a7fcb74a63542419adafb (MD5) LICENSE.txt: 4224 bytes, checksum: 08adbaa72186ac97044b8588a6f48600 (MD5) Previous issue date: 2016-12-09","Embargo set by: Seth Robbins for item 98742 Lift date: 2019-03-01T17:02:22Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 98742 Lift date: 2019-03-01T17:03:32Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 98742 Lift date: 2019-03-01T17:06:55Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 98742 on 2019-03-02T10:15:30Z."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/95626"],"dc:language":["en"],"dc:rights":["Copyright 2016 Gabriel V. Pereira"],"dc:subject":["Xylan degradation","Filamentous fungi","Heterologous expression","Biofuels","Bacteria","Bacteroides","Human gut","Fiber degradation"],"dc:title":["Xylan degradation in filamentous fungi and bacteria"],"dc:type":["text"],"thesis:degree_discipline":["Animal Sciences"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:37Z"}