{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/95620"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/95620","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The C-terminal domains of leucyl-tRNA synthetases","abstract":"The mitochondrial Leucyl-tRNA synthetase (mtLeuRS) of certain Caenorhabditis species contains an idiosyncratic C-terminal addition. Bioinformatic analyses identified this domain in the mtLeuRS and also four putative nuclear localization sequences (NLSs) encoded within the domain (Ezak, Hong, Chaparro-Garcia, & Ferkey, 2010). This C-terminal extension of Caenrhabditis mitochondrial LeuRS, or CECL domain, is highly positively charged and shows no obvious homologs in protein databases. Bioinformatic and computational modeling suggest that CECL is an added domain rather than being a part of the canonical C-terminal domain. An annotated splice-variant of the mitochondrial LeuRS hints at a mechanism for subcellular localization. Deletion of CECL from the chromosome prevents homozygous worms from surviving fertilization in most cases. From these observations, we hypothesize that the mitochondrial LeuRS of C. elegans performs an alternate nuclear function, perhaps carried out specifically in the neurons of the animal.","abstract_html":"The mitochondrial Leucyl-tRNA synthetase (mtLeuRS) of certain Caenorhabditis species contains an idiosyncratic C-terminal addition. Bioinformatic analyses identified this domain in the mtLeuRS and also four putative nuclear localization sequences (NLSs) encoded within the domain (Ezak, Hong, Chaparro-Garcia, & Ferkey, 2010). This C-terminal extension of Caenrhabditis mitochondrial LeuRS, or CECL domain, is highly positively charged and shows no obvious homologs in protein databases. Bioinformatic and computational modeling suggest that CECL is an added domain rather than being a part of the canonical C-terminal domain. An annotated splice-variant of the mitochondrial LeuRS hints at a mechanism for subcellular localization. Deletion of CECL from the chromosome prevents homozygous worms from surviving fertilization in most cases. From these observations, we hypothesize that the mitochondrial LeuRS of C. elegans performs an alternate nuclear function, perhaps carried out specifically in the neurons of the animal.","abstract_has_math":false,"creators":["Frimel, Aaron Michael"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Martinis, Susan A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-03-01T17:02:06Z","date_published":"2017-03-01T17:02:06Z","updated_at":"2026-07-22T22:26:37Z","subjects":["Leucyl-tRNA synthetase","Mitochondria","C. elegans"],"languages":["en"],"rights":["Copyright 2016 Aaron Frimel"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/95620","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Martinis, Susan A."]},{"key":"dc:creator","label":"Author","values":["Frimel, Aaron Michael"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2017-03-01T17:02:06Z","2019-03-02T10:15:14Z","2016-12-08","2016-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Leucyl-tRNA synthetase","Mitochondria","C. elegans"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2016 Aaron Frimel"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/95620"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The mitochondrial Leucyl-tRNA synthetase (mtLeuRS) of certain Caenorhabditis species contains an idiosyncratic C-terminal addition. Bioinformatic analyses identified this domain in the mtLeuRS and also four putative nuclear localization sequences (NLSs) encoded within the domain (Ezak, Hong, Chaparro-Garcia, & Ferkey, 2010). This C-terminal extension of Caenrhabditis mitochondrial LeuRS, or CECL domain, is highly positively charged and shows no obvious homologs in protein databases. Bioinformatic and computational modeling suggest that CECL is an added domain rather than being a part of the canonical C-terminal domain. An annotated splice-variant of the mitochondrial LeuRS hints at a mechanism for subcellular localization. Deletion of CECL from the chromosome prevents homozygous worms from surviving fertilization in most cases. From these observations, we hypothesize that the mitochondrial LeuRS of C. elegans performs an alternate nuclear function, perhaps carried out specifically in the neurons of the animal.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2018-12-01","The student, Aaron Frimel, accepted the attached license on 2016-12-07 at 13:02.","The student, Aaron Frimel, submitted this Thesis for approval on 2016-12-07 at 13:04.","This Thesis was approved for publication on 2016-12-08 at 16:13.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10471 on 2017-02-28 at 14:43:22","Made available in DSpace on 2017-03-01T17:02:06Z (GMT). No. of bitstreams: 2 FRIMEL-THESIS-2016.pdf: 2211377 bytes, checksum: 0cfe23660f539bd9b6d367c54c911149 (MD5) LICENSE.txt: 4209 bytes, checksum: 4acf17a29dbacc47e2057935d0f94658 (MD5) Previous issue date: 2016-12-08","Embargo set by: Seth Robbins for item 98736 Lift date: 2019-03-01T17:02:22Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 98736 Lift date: 2019-03-01T17:03:32Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 98736 Lift date: 2019-03-01T17:05:02Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 98736 Lift date: 2019-03-01T17:06:55Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 98736 on 2019-03-02T10:15:14Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The C-terminal domains of leucyl-tRNA synthetases"]}]}],"canonical_facts":{"dc:contributor":["Martinis, Susan A."],"dc:creator":["Frimel, Aaron Michael"],"dc:date":["2017-03-01T17:02:06Z","2019-03-02T10:15:14Z","2016-12-08","2016-12"],"dc:description":["The mitochondrial Leucyl-tRNA synthetase (mtLeuRS) of certain Caenorhabditis species contains an idiosyncratic C-terminal addition. Bioinformatic analyses identified this domain in the mtLeuRS and also four putative nuclear localization sequences (NLSs) encoded within the domain (Ezak, Hong, Chaparro-Garcia, & Ferkey, 2010). This C-terminal extension of Caenrhabditis mitochondrial LeuRS, or CECL domain, is highly positively charged and shows no obvious homologs in protein databases. Bioinformatic and computational modeling suggest that CECL is an added domain rather than being a part of the canonical C-terminal domain. An annotated splice-variant of the mitochondrial LeuRS hints at a mechanism for subcellular localization. Deletion of CECL from the chromosome prevents homozygous worms from surviving fertilization in most cases. From these observations, we hypothesize that the mitochondrial LeuRS of C. elegans performs an alternate nuclear function, perhaps carried out specifically in the neurons of the animal.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2018-12-01","The student, Aaron Frimel, accepted the attached license on 2016-12-07 at 13:02.","The student, Aaron Frimel, submitted this Thesis for approval on 2016-12-07 at 13:04.","This Thesis was approved for publication on 2016-12-08 at 16:13.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10471 on 2017-02-28 at 14:43:22","Made available in DSpace on 2017-03-01T17:02:06Z (GMT). No. of bitstreams: 2 FRIMEL-THESIS-2016.pdf: 2211377 bytes, checksum: 0cfe23660f539bd9b6d367c54c911149 (MD5) LICENSE.txt: 4209 bytes, checksum: 4acf17a29dbacc47e2057935d0f94658 (MD5) Previous issue date: 2016-12-08","Embargo set by: Seth Robbins for item 98736 Lift date: 2019-03-01T17:02:22Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 98736 Lift date: 2019-03-01T17:03:32Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 98736 Lift date: 2019-03-01T17:05:02Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 98736 Lift date: 2019-03-01T17:06:55Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 98736 on 2019-03-02T10:15:14Z."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/95620"],"dc:language":["en"],"dc:rights":["Copyright 2016 Aaron Frimel"],"dc:subject":["Leucyl-tRNA synthetase","Mitochondria","C. elegans"],"dc:title":["The C-terminal domains of leucyl-tRNA synthetases"],"dc:type":["text"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:37Z"}