{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/95604"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/95604","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Modification and regulation of biomolecules in vitro and in silico","abstract":"Made available in DSpace on 2017-03-01T17:01:53Z (GMT). No. of bitstreams: 4 CHAO-DISSERTATION-2016.pdf: 46584356 bytes, checksum: d3f45ec92d145f3f283e881146e0871b (MD5) JCE_Springer.pdf: 93928 bytes, checksum: e17be616416d3f9170c36fe13bf9811f (MD5) LICENSE.txt: 4209 bytes, checksum: b77af2c5bd4bd2671e6fb9b9632f01c6 (MD5) PROQUEST_LICENSE.txt: 4555 bytes, checksum: 6411e7773839884cb4f63464c8f7449a (MD5) Previous issue date: 2016-12-01","abstract_html":"Made available in DSpace on 2017-03-01T17:01:53Z (GMT). No. of bitstreams: 4 CHAO-DISSERTATION-2016.pdf: 46584356 bytes, checksum: d3f45ec92d145f3f283e881146e0871b (MD5) JCE_Springer.pdf: 93928 bytes, checksum: e17be616416d3f9170c36fe13bf9811f (MD5) LICENSE.txt: 4209 bytes, checksum: b77af2c5bd4bd2671e6fb9b9632f01c6 (MD5) PROQUEST_LICENSE.txt: 4555 bytes, checksum: 6411e7773839884cb4f63464c8f7449a (MD5) Previous issue date: 2016-12-01","abstract_has_math":false,"creators":["Chao, Shu-Han"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Physics","degree_department":null,"school":null,"contributors":["Gruebele, Martin","Aksimentiev, Aleksei","Tajkhorshid, Emad","Kuhlman, Thomas"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-03-01T17:01:53Z","date_published":"2017-03-01T17:01:53Z","updated_at":"2026-07-22T22:26:37Z","subjects":["DNA sequencing","molecular dynamics","nanopore","coarsed-grained model","double-nanopore","protein folding","PEGylation","thermal denaturation","Temperature Jump"],"languages":["en"],"rights":["Copyright 2016 Shu-Han Chao"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/95604","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Gruebele, Martin","Aksimentiev, Aleksei","Tajkhorshid, Emad","Kuhlman, Thomas"]},{"key":"dc:creator","label":"Author","values":["Chao, Shu-Han"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2017-03-01T17:01:53Z","2019-03-02T10:15:30Z","2016-12-01","2016-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physics"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["DNA sequencing","molecular dynamics","nanopore","coarsed-grained model","double-nanopore","protein folding","PEGylation","thermal denaturation","Temperature Jump"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2016 Shu-Han Chao"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/95604"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Made available in DSpace on 2017-03-01T17:01:53Z (GMT). No. of bitstreams: 4 CHAO-DISSERTATION-2016.pdf: 46584356 bytes, checksum: d3f45ec92d145f3f283e881146e0871b (MD5) JCE_Springer.pdf: 93928 bytes, checksum: e17be616416d3f9170c36fe13bf9811f (MD5) LICENSE.txt: 4209 bytes, checksum: b77af2c5bd4bd2671e6fb9b9632f01c6 (MD5) PROQUEST_LICENSE.txt: 4555 bytes, checksum: 6411e7773839884cb4f63464c8f7449a (MD5) Previous issue date: 2016-12-01","Embargo set by: Seth Robbins for item 98720 Lift date: 2019-03-01T17:02:22Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","The focus in functional and dynamics studies of biomolecules, such as protein or DNA, has been very much on their own structures and energy landscapes. However, in real biological systems, biomolecules are usually modified or regulated by several external factors, including surface coating, hydration condition, and local environment. For example, by covalently coupling a Poly(ethylene glycol) (PEG) on a protein surface, the stability of the host protein could be largely enhanced. This method, called PEGylation, has been widely used in pharmaceutical industry to protect protein drugs and increase their circulation life-time since the 1990s. However, the mechanism of protein-PEG interaction is yet to be understood. On the other hand, in developing one of the advanced DNA sequencing techniques --- nanopore sequencing, different mechanisms have been introduced to the nanopore system to regulate the conformation and motion of DNA molecules, attempting to achieve a better signal-to-noise ratio in reading DNA sequence. This dissertation aims to study the above two topics from both experiments and molecular dynamics simulations. The first part focuses on developing nanopore sequencing techniques. We have developed a method combining continuum modeling results of a nano-scale system and a coarse-grained DNA model to study the DNA translocation through a nanopore in a device scale. We use this method to develop advanced DNA sequencing techniques, including plasmonic nanopore and double nanopores. Both of them show great potential as novel approaches for DNA sequencing. The protein-PEG interaction is addressed in the second part. We show that the conjugated PEG affects the thermodynamic stability and local structure of the host protein WW domain, but not that of Lambda repressor. A reoccurring and cooperative folding of a PEG molecule onto the protein surface is revealed by molecular dynamics simulations. Specific PEG-binding motifs on the protein surface are identified.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2018-12-01","The student, Shu-Han Chao, accepted the attached license on 2016-12-01 at 11:15.","The student, Shu-Han Chao, submitted this Dissertation for approval on 2016-12-01 at 11:56.","This Dissertation was approved for publication on 2016-12-01 at 17:11.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10394 on 2017-02-28 at 14:42:44","Embargo set by: Seth Robbins for item 98720 Lift date: 2019-03-01T17:03:32Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 98720 Lift date: 2019-03-01T17:05:02Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 98720 Lift date: 2019-03-01T17:06:55Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 98720 on 2019-03-02T10:15:30Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Modification and regulation of biomolecules in vitro and in silico"]}]}],"canonical_facts":{"dc:contributor":["Gruebele, Martin","Aksimentiev, Aleksei","Tajkhorshid, Emad","Kuhlman, Thomas"],"dc:creator":["Chao, Shu-Han"],"dc:date":["2017-03-01T17:01:53Z","2019-03-02T10:15:30Z","2016-12-01","2016-12"],"dc:description":["Made available in DSpace on 2017-03-01T17:01:53Z (GMT). No. of bitstreams: 4 CHAO-DISSERTATION-2016.pdf: 46584356 bytes, checksum: d3f45ec92d145f3f283e881146e0871b (MD5) JCE_Springer.pdf: 93928 bytes, checksum: e17be616416d3f9170c36fe13bf9811f (MD5) LICENSE.txt: 4209 bytes, checksum: b77af2c5bd4bd2671e6fb9b9632f01c6 (MD5) PROQUEST_LICENSE.txt: 4555 bytes, checksum: 6411e7773839884cb4f63464c8f7449a (MD5) Previous issue date: 2016-12-01","Embargo set by: Seth Robbins for item 98720 Lift date: 2019-03-01T17:02:22Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","The focus in functional and dynamics studies of biomolecules, such as protein or DNA, has been very much on their own structures and energy landscapes. However, in real biological systems, biomolecules are usually modified or regulated by several external factors, including surface coating, hydration condition, and local environment. For example, by covalently coupling a Poly(ethylene glycol) (PEG) on a protein surface, the stability of the host protein could be largely enhanced. This method, called PEGylation, has been widely used in pharmaceutical industry to protect protein drugs and increase their circulation life-time since the 1990s. However, the mechanism of protein-PEG interaction is yet to be understood. On the other hand, in developing one of the advanced DNA sequencing techniques --- nanopore sequencing, different mechanisms have been introduced to the nanopore system to regulate the conformation and motion of DNA molecules, attempting to achieve a better signal-to-noise ratio in reading DNA sequence. This dissertation aims to study the above two topics from both experiments and molecular dynamics simulations. The first part focuses on developing nanopore sequencing techniques. We have developed a method combining continuum modeling results of a nano-scale system and a coarse-grained DNA model to study the DNA translocation through a nanopore in a device scale. We use this method to develop advanced DNA sequencing techniques, including plasmonic nanopore and double nanopores. Both of them show great potential as novel approaches for DNA sequencing. The protein-PEG interaction is addressed in the second part. We show that the conjugated PEG affects the thermodynamic stability and local structure of the host protein WW domain, but not that of Lambda repressor. A reoccurring and cooperative folding of a PEG molecule onto the protein surface is revealed by molecular dynamics simulations. Specific PEG-binding motifs on the protein surface are identified.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2018-12-01","The student, Shu-Han Chao, accepted the attached license on 2016-12-01 at 11:15.","The student, Shu-Han Chao, submitted this Dissertation for approval on 2016-12-01 at 11:56.","This Dissertation was approved for publication on 2016-12-01 at 17:11.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10394 on 2017-02-28 at 14:42:44","Embargo set by: Seth Robbins for item 98720 Lift date: 2019-03-01T17:03:32Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 98720 Lift date: 2019-03-01T17:05:02Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 98720 Lift date: 2019-03-01T17:06:55Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 98720 on 2019-03-02T10:15:30Z."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/95604"],"dc:language":["en"],"dc:rights":["Copyright 2016 Shu-Han Chao"],"dc:subject":["DNA sequencing","molecular dynamics","nanopore","coarsed-grained model","double-nanopore","protein folding","PEGylation","thermal denaturation","Temperature Jump"],"dc:title":["Modification and regulation of biomolecules in vitro and in silico"],"dc:type":["text"],"thesis:degree_discipline":["Physics"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:37Z"}