Endocannabinoid and lipid metabolism genes network analysis in adipose and liver tissue of dairy cows during the transition period
type: CreativeWork
description: During the transition period dairy cows are exposed to enormous metabolic changes. These changes could affect the overall health and production. Two experiments were conducted on transition cows to evaluate the 1) effects of body condition score (BCS) on the endocannabinoid system and lipid metabolism gene expression in adipose tissue; and 2) effects of rumen-protected methionine on the endocannabinoid system in liver tissue. In the first study, cows were retrospectively classified according to their BCS at -3 wk from parturition into two groups: HiBCS (BCS ≥ 3.75) or LoBCS (BCS ≤ 3.25 ). Adipose tissue at -10, 7, and 20 d around parturition was used to examine mRNA expression via qPCR of endocannabinoid receptors (CNR1, CNR2), enzymes that synthesize endocannabinoid (NAPEPLD), enzymes that degrade endocannabinoids (FAAH, NAAA, MGLL), and the hormone precursor proopiomelanocortin (POMC). We also examined mRNA expression via qPCR of genes associated with lipolysis (LIPE, ABDH5, ATGL), fatty acid oxidation (CPT1A, CPT2, ACADVL, ACOX1), oxidative stress (SOD1, SOD2), and genes that are involved in inflammation (TLR9, TLR4, NFE2L2). Expression of CNR2 and NAPEPLD was greater at 7 d in LoBCS due to lower expression at the same time in HiBCS. The expression of FAAH was upregulated at d 7 and 20 in LoBCS than HiBCS cows. Expression of MGLL was overall greater across time in LoBCS than HiBCS, LoBCS had a tendency for greater overall expression of POMC across time. Regarding the genes associated with lipolysis, LoBCS compared with HiBCS cows had overall greater expression of ABDH5, LIPE and ATGL, indicating a greater state of basal lipolysis over time. Among genes related with fatty acid oxidation the expression of CPT1A and ACADVL was greater in HiBCS than LoBCS due to greater expression at -10 and 7 d. For the mitochondrial enzyme SOD2, important for clearing reactive-oxygen species that cause cellular stress and inflammation, we observed an interaction of BCS × day due to…
abstract: During the transition period dairy cows are exposed to enormous metabolic changes. These changes could affect the overall health and production. Two experiments were conducted on transition cows to evaluate the 1) effects of body condition score (BCS) on the endocannabinoid system and lipid metabolism gene expression in adipose tissue; and 2) effects of rumen-protected methionine on the endocannabinoid system in liver tissue. In the first study, cows were retrospectively classified according to their BCS at -3 wk from parturition into two groups: HiBCS (BCS ≥ 3.75) or LoBCS (BCS ≤ 3.25 ). Adipose tissue at -10, 7, and 20 d around parturition was used to examine mRNA expression via qPCR of endocannabinoid receptors (CNR1, CNR2), enzymes that synthesize endocannabinoid (NAPEPLD), enzymes that degrade endocannabinoids (FAAH, NAAA, MGLL), and the hormone precursor proopiomelanocortin (POMC). We also examined mRNA expression via qPCR of genes associated with lipolysis (LIPE, ABDH5, ATGL), fatty acid oxidation (CPT1A, CPT2, ACADVL, ACOX1), oxidative stress (SOD1, SOD2), and genes that are involved in inflammation (TLR9, TLR4, NFE2L2). Expression of CNR2 and NAPEPLD was greater at 7 d in LoBCS due to lower expression at the same time in HiBCS. The expression of FAAH was upregulated at d 7 and 20 in LoBCS than HiBCS cows. Expression of MGLL was overall greater across time in LoBCS than HiBCS, LoBCS had a tendency for greater overall expression of POMC across time. Regarding the genes associated with lipolysis, LoBCS compared with HiBCS cows had overall greater expression of ABDH5, LIPE and ATGL, indicating a greater state of basal lipolysis over time. Among genes related with fatty acid oxidation the expression of CPT1A and ACADVL was greater in HiBCS than LoBCS due to greater expression at -10 and 7 d. For the mitochondrial enzyme SOD2, important for clearing reactive-oxygen species that cause cellular stress and inflammation, we observed an interaction of BCS × day due to…
author: Alharthi, Abdulrahman Salem M
language: en
keywords: Endocannabinoid, Transition period, Lipid metabolism, Body condition score
date published: 2017-03-01T17:00:51Z
date modified: 2026-07-22T22:26:37Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/95542