The effects of equine plasma transfusion on markers of inflammation in healthy neonatal foals
type: CreativeWork
description: Very few studies in veterinary medicine have investigated the immunologic changes and inflammatory markers associated with transfusion of fresh frozen plasma in neonatal foals. Plasma transfusion is routinely used in the equine neonate for several reasons ranging from prophylaxis of disease to complementary treatment of ongoing conditions. Previous studies in hospitalized sick neonatal foals have reported a transfusion reaction rate close to 10% (Hardefeldt, 2010). In this prospective study, we examined the immunological impact of intravenous (IV) fresh frozen plasma administration in healthy newborn foals, using a three-fold approach. Systemic, plasma-invoked immune and inflammatory responses were assessed by measuring 1) peripheral blood leukocytes cell cytokine gene expression, 2) circulating cytokine concentration (interferon-gamma [IFN-γ], interleukin- 1β [IL-1 β], tumor necrosis factor-α [TNF- α], interleukin-6 [IL-6], interleukin-10 [IL-10] and interleukin-8 [IL-8], interleukin-4 [IL-4], interleukin-17 [IL-17]), and 3) changes in acute phase protein (fibrinogen [FIB] and serum amyloid A [SAA]) production. A total of 35 healthy, neonatal foals (mean ± SD age 34 ± 5 hours) were randomly allocated into one of two groups, and given either hyperimmune, fresh frozen plasma (treatment) or crystalloids (control) by IV administration through a preplaced jugular catheter. Blood samples were collected before (hour 0) treatment and at 2, 6 and 24 hours after transfusion. Serum cytokines concentrations were measured with either bead based multiplex technology (IFN-γ, IL-4, IL-17, IL-10) or with commercial ELISA kits (IL-6, IL-8 and IL-1β, TNF- α, IL-10). Peripheral blood leukocyte gene expression was performed with qRT-PCR. Serum amyloid A [SAA] was measured with turbidometric immunoassay and fibrinogen concentration [FIB] was determined with the method of Clauss. Comparisons between groups were made using a Kruskal Wallis test, and changes overtime within groups were ana…
abstract: Very few studies in veterinary medicine have investigated the immunologic changes and inflammatory markers associated with transfusion of fresh frozen plasma in neonatal foals. Plasma transfusion is routinely used in the equine neonate for several reasons ranging from prophylaxis of disease to complementary treatment of ongoing conditions. Previous studies in hospitalized sick neonatal foals have reported a transfusion reaction rate close to 10% (Hardefeldt, 2010). In this prospective study, we examined the immunological impact of intravenous (IV) fresh frozen plasma administration in healthy newborn foals, using a three-fold approach. Systemic, plasma-invoked immune and inflammatory responses were assessed by measuring 1) peripheral blood leukocytes cell cytokine gene expression, 2) circulating cytokine concentration (interferon-gamma [IFN-γ], interleukin- 1β [IL-1 β], tumor necrosis factor-α [TNF- α], interleukin-6 [IL-6], interleukin-10 [IL-10] and interleukin-8 [IL-8], interleukin-4 [IL-4], interleukin-17 [IL-17]), and 3) changes in acute phase protein (fibrinogen [FIB] and serum amyloid A [SAA]) production. A total of 35 healthy, neonatal foals (mean ± SD age 34 ± 5 hours) were randomly allocated into one of two groups, and given either hyperimmune, fresh frozen plasma (treatment) or crystalloids (control) by IV administration through a preplaced jugular catheter. Blood samples were collected before (hour 0) treatment and at 2, 6 and 24 hours after transfusion. Serum cytokines concentrations were measured with either bead based multiplex technology (IFN-γ, IL-4, IL-17, IL-10) or with commercial ELISA kits (IL-6, IL-8 and IL-1β, TNF- α, IL-10). Peripheral blood leukocyte gene expression was performed with qRT-PCR. Serum amyloid A [SAA] was measured with turbidometric immunoassay and fibrinogen concentration [FIB] was determined with the method of Clauss. Comparisons between groups were made using a Kruskal Wallis test, and changes overtime within groups were ana…
author: Po, Eleonora
language: en
keywords: Foal, Equine, Fresh Frozen Plasma: Cytokines, Acute phase proteins
date published: 2017-03-01T16:36:35Z
date modified: 2026-07-22T22:26:37Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/95443