{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/92994"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/92994","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Methodologies for the analysis of membrane systems using lipid nanodiscs","abstract":"The student, Ivan Lenov, accepted the attached license on 2016-05-24 at 11:01.","abstract_html":"The student, Ivan Lenov, accepted the attached license on 2016-05-24 at 11:01.","abstract_has_math":false,"creators":["Lenov, Ivan Lenkov"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Sligar, Stephen G.","Bailey, Ryan C.","Murphy, Catherine J.","Morrissey, James H."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-11-10T18:39:02Z","date_published":"2016-11-10T18:39:02Z","updated_at":"2026-07-22T22:26:35Z","subjects":["Nanodiscs","lipids","membrane","membrane protein","Cytochrome P450"],"languages":["en"],"rights":["Copyright 2016 Ivan Lenkov Lenov"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/92994","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Sligar, Stephen G.","Bailey, Ryan C.","Murphy, Catherine J.","Morrissey, James H."]},{"key":"dc:creator","label":"Author","values":["Lenov, Ivan Lenkov"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2016-11-10T18:39:02Z","2018-11-11T10:15:11Z","2016-05-25","2016-08"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Nanodiscs","lipids","membrane","membrane protein","Cytochrome P450"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2016 Ivan Lenkov Lenov"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/92994"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The student, Ivan Lenov, accepted the attached license on 2016-05-24 at 11:01.","The student, Ivan Lenov, submitted this Dissertation for approval on 2016-05-24 at 11:05.","This Dissertation was approved for publication on 2016-05-25 at 14:53.","DSpace SAF Submission Ingestion Package generated from Vireo submission #9625 on 2016-11-10 at 12:24:17","Made available in DSpace on 2016-11-10T18:39:02Z (GMT). No. of bitstreams: 3 LENOV-DISSERTATION-2016.pdf: 6019364 bytes, checksum: cea3c1b7c442b902ae9616576be545b0 (MD5) LICENSE.txt: 4207 bytes, checksum: 50e73ff6c33b7bda656466b98a886860 (MD5) RIghts Nanodiscs.pdf: 141262 bytes, checksum: a612dc98038d65a58bcc78044c9f56d8 (MD5) Previous issue date: 2016-05-25","Membrane proteins are biologically significant targets of study due to their crucial roles in biochemical reactions, such as ion transport and cell signaling. Their study, however, is hampered by hydrophobic regions in their structures which cause aggregation without the presence of a membrane. For this reason, solubilization systems have been developed, but there are limitations to most. Nanodiscs were developed as an alternative platform that provides a native-like lipid bilayer for solubilizing membrane proteins with unparalleled control over lipid composition, exceptional monodispersity, and exceptional modularity. This dissertation details the coupling of Nanodiscs to multiple analytical platforms for the characterization of membrane systems, including Cytochrome P450s, blood coagulation factor proteins, and other membrane protein targets. The topology of three different Cytochrome P450 systems was characterized using linear dichroism spectroscopy. Methods were developed for the coupling of Nanodiscs to two different types of photonic biosensors, in atmospheric pressure and high pressure environments. The work further details the development of a microfluidic platform for the optimization of membrane protein incorporation into Nanodiscs as well as the development of a bimodal imaging construct utilizing Nanodiscs and a Gd(III) chelating molecule used as a contrast agent for the labeling of cells.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2018-08-01","Embargo set by: Seth Robbins for item 95416 Lift date: 2018-11-10T18:39:22Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 95416 Lift date: 2018-11-10T18:43:22Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only Restriction Lifted for Item 95416 on 2018-11-11T10:15:11Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Methodologies for the analysis of membrane systems using lipid nanodiscs"]}]}],"canonical_facts":{"dc:contributor":["Sligar, Stephen G.","Bailey, Ryan C.","Murphy, Catherine J.","Morrissey, James H."],"dc:creator":["Lenov, Ivan Lenkov"],"dc:date":["2016-11-10T18:39:02Z","2018-11-11T10:15:11Z","2016-05-25","2016-08"],"dc:description":["The student, Ivan Lenov, accepted the attached license on 2016-05-24 at 11:01.","The student, Ivan Lenov, submitted this Dissertation for approval on 2016-05-24 at 11:05.","This Dissertation was approved for publication on 2016-05-25 at 14:53.","DSpace SAF Submission Ingestion Package generated from Vireo submission #9625 on 2016-11-10 at 12:24:17","Made available in DSpace on 2016-11-10T18:39:02Z (GMT). No. of bitstreams: 3 LENOV-DISSERTATION-2016.pdf: 6019364 bytes, checksum: cea3c1b7c442b902ae9616576be545b0 (MD5) LICENSE.txt: 4207 bytes, checksum: 50e73ff6c33b7bda656466b98a886860 (MD5) RIghts Nanodiscs.pdf: 141262 bytes, checksum: a612dc98038d65a58bcc78044c9f56d8 (MD5) Previous issue date: 2016-05-25","Membrane proteins are biologically significant targets of study due to their crucial roles in biochemical reactions, such as ion transport and cell signaling. Their study, however, is hampered by hydrophobic regions in their structures which cause aggregation without the presence of a membrane. For this reason, solubilization systems have been developed, but there are limitations to most. Nanodiscs were developed as an alternative platform that provides a native-like lipid bilayer for solubilizing membrane proteins with unparalleled control over lipid composition, exceptional monodispersity, and exceptional modularity. This dissertation details the coupling of Nanodiscs to multiple analytical platforms for the characterization of membrane systems, including Cytochrome P450s, blood coagulation factor proteins, and other membrane protein targets. The topology of three different Cytochrome P450 systems was characterized using linear dichroism spectroscopy. Methods were developed for the coupling of Nanodiscs to two different types of photonic biosensors, in atmospheric pressure and high pressure environments. The work further details the development of a microfluidic platform for the optimization of membrane protein incorporation into Nanodiscs as well as the development of a bimodal imaging construct utilizing Nanodiscs and a Gd(III) chelating molecule used as a contrast agent for the labeling of cells.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2018-08-01","Embargo set by: Seth Robbins for item 95416 Lift date: 2018-11-10T18:39:22Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 95416 Lift date: 2018-11-10T18:43:22Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only Restriction Lifted for Item 95416 on 2018-11-11T10:15:11Z."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/92994"],"dc:language":["en"],"dc:rights":["Copyright 2016 Ivan Lenkov Lenov"],"dc:subject":["Nanodiscs","lipids","membrane","membrane protein","Cytochrome P450"],"dc:title":["Methodologies for the analysis of membrane systems using lipid nanodiscs"],"dc:type":["text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:35Z"}