Defining the role of toll-like receptor 10 in immune signaling
type: CreativeWork
description: Toll-like Receptors (TLRs) represent a class of pattern recognition receptors that function to recognize invading microbes and initiate pro-inflammatory responses. TLR10 is the only remaining orphan member of the human TLR family. Given the established role of TLR signaling in both the innate and adaptive arms of immune defense, defining the function of TLR10 in the immune response would fill a significant knowledge gap regarding the question of why the gene has been propagated through evolutionary history. Additionally, given the growing recognition of the impact of TLR biology on human disease, additional knowledge of this orphan TLR may also provide a novel therapeutic target. Chapter One of this dissertation introduces the main events that occur in the innate and adaptive immune responses to infection and describes the role of TLRs in the induction and propagation of such responses. It also highlights the growing recognition of lymphocyte-intrinsic TLR signaling and its role in robust adaptive immune responses. Lastly, it provides background information on what is known about TLR10 currently and identifies the knowledge gap that this research seeks to fill. Chapter Two describes the capacity of TLR10 to act as a general suppressor of pro-inflammatory TLR signaling. Given the lack of TLR10 expression in mice, heterologous expression of TLR10 in cell lines and transgenic mice was used, and these studies revealed an inhibitory role for TLR10 in the context of pro-inflammatory cellular responses to numerous TLR ligands. The effect of TLR10 in abrogating both MyD88 and TRIF dependent signaling was discovered using transient transfection techniques. Lastly, ligation of cell surface TLR10 in whole blood using a monoclonal antibody resulted in strongly attenuated responses to TLR agonists (Jiang et al. 2016). Chapter Three focuses on the expression of surface TLR10 within the B cell lineage. While numerous studies have shown the presence of TLR10 mRNA in cell lines and …
abstract: Toll-like Receptors (TLRs) represent a class of pattern recognition receptors that function to recognize invading microbes and initiate pro-inflammatory responses. TLR10 is the only remaining orphan member of the human TLR family. Given the established role of TLR signaling in both the innate and adaptive arms of immune defense, defining the function of TLR10 in the immune response would fill a significant knowledge gap regarding the question of why the gene has been propagated through evolutionary history. Additionally, given the growing recognition of the impact of TLR biology on human disease, additional knowledge of this orphan TLR may also provide a novel therapeutic target. Chapter One of this dissertation introduces the main events that occur in the innate and adaptive immune responses to infection and describes the role of TLRs in the induction and propagation of such responses. It also highlights the growing recognition of lymphocyte-intrinsic TLR signaling and its role in robust adaptive immune responses. Lastly, it provides background information on what is known about TLR10 currently and identifies the knowledge gap that this research seeks to fill. Chapter Two describes the capacity of TLR10 to act as a general suppressor of pro-inflammatory TLR signaling. Given the lack of TLR10 expression in mice, heterologous expression of TLR10 in cell lines and transgenic mice was used, and these studies revealed an inhibitory role for TLR10 in the context of pro-inflammatory cellular responses to numerous TLR ligands. The effect of TLR10 in abrogating both MyD88 and TRIF dependent signaling was discovered using transient transfection techniques. Lastly, ligation of cell surface TLR10 in whole blood using a monoclonal antibody resulted in strongly attenuated responses to TLR agonists (Jiang et al. 2016). Chapter Three focuses on the expression of surface TLR10 within the B cell lineage. While numerous studies have shown the presence of TLR10 mRNA in cell lines and …
author: Jiang, Song
language: en
keywords: Toll-like Receptor 10, TLR, Immune signaling
date published: 2016-11-10T18:27:25Z
date modified: 2026-07-22T22:26:35Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/92894