{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/92880"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/92880","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Method development for the characterization of intrinsic and extant kinetic parameters in microalgal cultures","abstract":"Algal bioprocesses for wastewater treatment have the potential to simultaneously recover nitrogen and phosphorous while also producing feedstocks for bioenergy and bioproducts. In order to develop reliable treatment processes for water resource recovery facilities, however, it is critical that we have robust, mechanistic models of phototrophic bioprocesses to predict performance under varying environmental conditions (light intensity, nutrient concentration, etc.). As we seek to develop such models, we must also have reliable approaches to calibrate and validate key parameters that govern model accuracy. The objectives of this research are to develop an experimental apparatus and methodology to characterize intrinsic and extant kinetic parameters in phototrophic cultures. Intrinsic kinetic parameters – used in model calibration – represent the maximum kinetic potential of the culture. Extant kinetic parameters – used in model validation – are representative of the existing fitness and performance of the biomass in the bioreactor. A temperature-controlled system with ten independent light chambers was designed and fabricated to enable kinetic experiments with four replicate vials within each chamber, with lighting intensity in each chamber independently controlled. The apparatus was demonstrated to enable characterization of growth rate, nitrogen uptake, and phosphorus uptake. Quantification of these parameters will help to identify, characterize, and validate mechanistic links between process design, environmental conditions, nutrient recovery, and community function.","abstract_html":"Algal bioprocesses for wastewater treatment have the potential to simultaneously recover nitrogen and phosphorous while also producing feedstocks for bioenergy and bioproducts. In order to develop reliable treatment processes for water resource recovery facilities, however, it is critical that we have robust, mechanistic models of phototrophic bioprocesses to predict performance under varying environmental conditions (light intensity, nutrient concentration, etc.). As we seek to develop such models, we must also have reliable approaches to calibrate and validate key parameters that govern model accuracy. The objectives of this research are to develop an experimental apparatus and methodology to characterize intrinsic and extant kinetic parameters in phototrophic cultures. Intrinsic kinetic parameters – used in model calibration – represent the maximum kinetic potential of the culture. Extant kinetic parameters – used in model validation – are representative of the existing fitness and performance of the biomass in the bioreactor. A temperature-controlled system with ten independent light chambers was designed and fabricated to enable kinetic experiments with four replicate vials within each chamber, with lighting intensity in each chamber independently controlled. The apparatus was demonstrated to enable characterization of growth rate, nitrogen uptake, and phosphorus uptake. Quantification of these parameters will help to identify, characterize, and validate mechanistic links between process design, environmental conditions, nutrient recovery, and community function.","abstract_has_math":false,"creators":["Lardizabal, Amanda"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Environ Engr in Civil Engr","degree_department":null,"school":null,"contributors":["Guest, Jeremy"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-11-10T18:27:13Z","date_published":"2016-11-10T18:27:13Z","updated_at":"2026-07-22T22:26:35Z","subjects":["Modeling","Calibration","Batch","Photosynthesis","Protein synthesis inhibition"],"languages":["en"],"rights":["Copyright 2016 Amanda Lardizabal"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/92880","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Guest, Jeremy"]},{"key":"dc:creator","label":"Author","values":["Lardizabal, Amanda"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2016-11-10T18:27:13Z","2018-11-11T10:15:12Z","2016-07-22","2016-08"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Environ Engr in Civil Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Modeling","Calibration","Batch","Photosynthesis","Protein synthesis inhibition"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2016 Amanda Lardizabal"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/92880"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Algal bioprocesses for wastewater treatment have the potential to simultaneously recover nitrogen and phosphorous while also producing feedstocks for bioenergy and bioproducts. In order to develop reliable treatment processes for water resource recovery facilities, however, it is critical that we have robust, mechanistic models of phototrophic bioprocesses to predict performance under varying environmental conditions (light intensity, nutrient concentration, etc.). As we seek to develop such models, we must also have reliable approaches to calibrate and validate key parameters that govern model accuracy. The objectives of this research are to develop an experimental apparatus and methodology to characterize intrinsic and extant kinetic parameters in phototrophic cultures. Intrinsic kinetic parameters – used in model calibration – represent the maximum kinetic potential of the culture. Extant kinetic parameters – used in model validation – are representative of the existing fitness and performance of the biomass in the bioreactor. A temperature-controlled system with ten independent light chambers was designed and fabricated to enable kinetic experiments with four replicate vials within each chamber, with lighting intensity in each chamber independently controlled. The apparatus was demonstrated to enable characterization of growth rate, nitrogen uptake, and phosphorus uptake. Quantification of these parameters will help to identify, characterize, and validate mechanistic links between process design, environmental conditions, nutrient recovery, and community function.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2018-08-01","The student, Amanda Lardizabal, accepted the attached license on 2016-07-22 at 08:26.","The student, Amanda Lardizabal, submitted this Thesis for approval on 2016-07-22 at 08:38.","This Thesis was approved for publication on 2016-07-22 at 11:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10070 on 2016-11-10 at 12:21:11","Made available in DSpace on 2016-11-10T18:27:13Z (GMT). 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In order to develop reliable treatment processes for water resource recovery facilities, however, it is critical that we have robust, mechanistic models of phototrophic bioprocesses to predict performance under varying environmental conditions (light intensity, nutrient concentration, etc.). As we seek to develop such models, we must also have reliable approaches to calibrate and validate key parameters that govern model accuracy. The objectives of this research are to develop an experimental apparatus and methodology to characterize intrinsic and extant kinetic parameters in phototrophic cultures. Intrinsic kinetic parameters – used in model calibration – represent the maximum kinetic potential of the culture. Extant kinetic parameters – used in model validation – are representative of the existing fitness and performance of the biomass in the bioreactor. A temperature-controlled system with ten independent light chambers was designed and fabricated to enable kinetic experiments with four replicate vials within each chamber, with lighting intensity in each chamber independently controlled. The apparatus was demonstrated to enable characterization of growth rate, nitrogen uptake, and phosphorus uptake. Quantification of these parameters will help to identify, characterize, and validate mechanistic links between process design, environmental conditions, nutrient recovery, and community function.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2018-08-01","The student, Amanda Lardizabal, accepted the attached license on 2016-07-22 at 08:26.","The student, Amanda Lardizabal, submitted this Thesis for approval on 2016-07-22 at 08:38.","This Thesis was approved for publication on 2016-07-22 at 11:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #10070 on 2016-11-10 at 12:21:11","Made available in DSpace on 2016-11-10T18:27:13Z (GMT). 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