{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/90964"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/90964","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Proliferation and morphology of pre-osteoblast cells on 25 – 1000 micron diameter patterned features on polyacrylamide hydrogels","abstract":"Bone formation, also known as osteogenesis, occurs in three distinct phases: proliferation, during which cells multiply, form nodules, and begin expressing early stage osteogenic markers; extracellular matrix development and maturation, during which mature cells in nodules build a collagen matrix and secrete the building blocks for mineralization; and mineralization, during which bone mineral crystals nucleate and grow. While it is known that cell proliferation, maturation, morphology, and density all play a role of in osteogenesis, most studies of controlled cell differentiation either focus proliferation and maturation in large cell colonies or morphology of single cells. However, large cell colonies are difficult to examine because of the presence of multiple bone nodules and mineral nucleation sites, and the single cell differentiation experiments ignore the importance of cell proliferation, cell maturation, and cell-cell contact/interaction. In this work, microcontact printing is used to pattern 19 kPa hydrogel substrates with 1000, 500, 250, 100, 50, and 25 micron diameter circles of fibronectin and collagen. Experiments were conducted in which cell proliferation of D1 ORL UVA cells was measured as the cells filled the patterns. Cell seeding density and cell density at confluence do not depend on media type or feature size, as long as cells are not confined to a small area (25 micron). The fold increase is significantly higher for larger features, where the cells have ample area to rapidly proliferate, but not significant for smaller features where the cells do not rapidly proliferate. Proliferating cells have an elongated morphology and mature cells at confluence are cuboidal. In future work, quantitative cell shape, proliferation, and early osteogenic and adipogenic marker data will be combined into a simulation to further study osteogenesis.","abstract_html":"Bone formation, also known as osteogenesis, occurs in three distinct phases: proliferation, during which cells multiply, form nodules, and begin expressing early stage osteogenic markers; extracellular matrix development and maturation, during which mature cells in nodules build a collagen matrix and secrete the building blocks for mineralization; and mineralization, during which bone mineral crystals nucleate and grow. While it is known that cell proliferation, maturation, morphology, and density all play a role of in osteogenesis, most studies of controlled cell differentiation either focus proliferation and maturation in large cell colonies or morphology of single cells. However, large cell colonies are difficult to examine because of the presence of multiple bone nodules and mineral nucleation sites, and the single cell differentiation experiments ignore the importance of cell proliferation, cell maturation, and cell-cell contact/interaction. In this work, microcontact printing is used to pattern 19 kPa hydrogel substrates with 1000, 500, 250, 100, 50, and 25 micron diameter circles of fibronectin and collagen. Experiments were conducted in which cell proliferation of D1 ORL UVA cells was measured as the cells filled the patterns. Cell seeding density and cell density at confluence do not depend on media type or feature size, as long as cells are not confined to a small area (25 micron). The fold increase is significantly higher for larger features, where the cells have ample area to rapidly proliferate, but not significant for smaller features where the cells do not rapidly proliferate. Proliferating cells have an elongated morphology and mature cells at confluence are cuboidal. In future work, quantitative cell shape, proliferation, and early osteogenic and adipogenic marker data will be combined into a simulation to further study osteogenesis.","abstract_has_math":false,"creators":["Berent, Zachary T"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Mechanical Engineering","degree_department":null,"school":null,"contributors":["Wagoner Johnson, Amy J."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-07-07T21:18:06Z","date_published":"2016-07-07T21:18:06Z","updated_at":"2026-07-22T22:26:34Z","subjects":["osteogenesis","microcontact printing"],"languages":["en"],"rights":["© 2016 Zachary Taylor Berent"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/90964","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Wagoner Johnson, Amy J."]},{"key":"dc:creator","label":"Author","values":["Berent, Zachary T"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2016-07-07T21:18:06Z","2018-07-08T09:15:16Z","2016-04-27","2016-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Mechanical Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["osteogenesis","microcontact printing"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["© 2016 Zachary Taylor Berent"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/90964"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Bone formation, also known as osteogenesis, occurs in three distinct phases: proliferation, during which cells multiply, form nodules, and begin expressing early stage osteogenic markers; extracellular matrix development and maturation, during which mature cells in nodules build a collagen matrix and secrete the building blocks for mineralization; and mineralization, during which bone mineral crystals nucleate and grow. While it is known that cell proliferation, maturation, morphology, and density all play a role of in osteogenesis, most studies of controlled cell differentiation either focus proliferation and maturation in large cell colonies or morphology of single cells. However, large cell colonies are difficult to examine because of the presence of multiple bone nodules and mineral nucleation sites, and the single cell differentiation experiments ignore the importance of cell proliferation, cell maturation, and cell-cell contact/interaction. In this work, microcontact printing is used to pattern 19 kPa hydrogel substrates with 1000, 500, 250, 100, 50, and 25 micron diameter circles of fibronectin and collagen. Experiments were conducted in which cell proliferation of D1 ORL UVA cells was measured as the cells filled the patterns. Cell seeding density and cell density at confluence do not depend on media type or feature size, as long as cells are not confined to a small area (25 micron). The fold increase is significantly higher for larger features, where the cells have ample area to rapidly proliferate, but not significant for smaller features where the cells do not rapidly proliferate. Proliferating cells have an elongated morphology and mature cells at confluence are cuboidal. In future work, quantitative cell shape, proliferation, and early osteogenic and adipogenic marker data will be combined into a simulation to further study osteogenesis.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2018-05-01","The student, Zachary Berent, accepted the attached license on 2016-04-26 at 14:40.","The student, Zachary Berent, submitted this Thesis for approval on 2016-04-26 at 14:49.","This Thesis was approved for publication on 2016-04-27 at 09:08.","DSpace SAF Submission Ingestion Package generated from Vireo submission #9518 on 2016-07-07 at 14:18:03","Made available in DSpace on 2016-07-07T21:18:06Z (GMT). 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While it is known that cell proliferation, maturation, morphology, and density all play a role of in osteogenesis, most studies of controlled cell differentiation either focus proliferation and maturation in large cell colonies or morphology of single cells. However, large cell colonies are difficult to examine because of the presence of multiple bone nodules and mineral nucleation sites, and the single cell differentiation experiments ignore the importance of cell proliferation, cell maturation, and cell-cell contact/interaction. In this work, microcontact printing is used to pattern 19 kPa hydrogel substrates with 1000, 500, 250, 100, 50, and 25 micron diameter circles of fibronectin and collagen. Experiments were conducted in which cell proliferation of D1 ORL UVA cells was measured as the cells filled the patterns. Cell seeding density and cell density at confluence do not depend on media type or feature size, as long as cells are not confined to a small area (25 micron). The fold increase is significantly higher for larger features, where the cells have ample area to rapidly proliferate, but not significant for smaller features where the cells do not rapidly proliferate. Proliferating cells have an elongated morphology and mature cells at confluence are cuboidal. In future work, quantitative cell shape, proliferation, and early osteogenic and adipogenic marker data will be combined into a simulation to further study osteogenesis.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2018-05-01","The student, Zachary Berent, accepted the attached license on 2016-04-26 at 14:40.","The student, Zachary Berent, submitted this Thesis for approval on 2016-04-26 at 14:49.","This Thesis was approved for publication on 2016-04-27 at 09:08.","DSpace SAF Submission Ingestion Package generated from Vireo submission #9518 on 2016-07-07 at 14:18:03","Made available in DSpace on 2016-07-07T21:18:06Z (GMT). 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