{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/90920"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/90920","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Skeletal muscle pericyte response to acute and chronic electrical stimulation","abstract":"Pericytes are mural cells that are located on the outer surface of blood capillaries where they attach to endothelial cells and regulate vascular function, including dilation and angiogenesis. Recent studies suggest that pericytes in skeletal muscle may contribute to myofiber repair in response to injury. However, the pericyte response to exercise remains largely unexplored. PURPOSE: The purpose of this study was to evaluate pericyte quantity and gene expression in skeletal muscle following electrical stimulation, a method that can be used to simulate resistance exercise in mice. METHODS: Adult wild-type mice were subjected to an electrical stimulation protocol that results in 20 eccentric and 20 concentric contractions during a single session or (n=5; n=4 sham). A separate cohort of mixed-sex mice were subjected to electrical stimulation twice weekly for 4 weeks (n=4; n=4 sham) and 9 weeks (n=3; n=3 sham). At the end of each study, gastrocnemius-soleus complexes were dissected 24h following the final bout of stimulation. Pericyte quantity was assessed by multiplex flow cytometry in all samples. NG2+CD45-CD31- and CD146+CD45-CD31- pericytes were isolated following the acute study and gene expression was evaluated using high throughput qPCR. RESULTS: Acute electrical stimulation resulted in a non-significant trend for an increase in total pericyte content in skeletal muscle and a significant increase in the percentage of NG2+CD45-CD31- pericytes expressing the mesenchymal stem/stromal cell (MSC) marker CD140A. Isolation of pericytes based on CD146 revealed a population of cells highly engaged in the synthesis of factors necessary for myogenesis, satellite cell activation, and extracellular matrix remodeling post-acute stimulation. Finally, a pericyte to MSC transition was also observed with 4 weeks of stimulation, but no changes in overall pericyte quantity were noted at 4 or 9 weeks. CONCLUSION: This study provides evidence that resistance exercise promotes a pericyte to MSC transition, an event that may be necessary for pericytes to engage in skeletal muscle repair and adaptation.","abstract_html":"Pericytes are mural cells that are located on the outer surface of blood capillaries where they attach to endothelial cells and regulate vascular function, including dilation and angiogenesis. Recent studies suggest that pericytes in skeletal muscle may contribute to myofiber repair in response to injury. However, the pericyte response to exercise remains largely unexplored. PURPOSE: The purpose of this study was to evaluate pericyte quantity and gene expression in skeletal muscle following electrical stimulation, a method that can be used to simulate resistance exercise in mice. METHODS: Adult wild-type mice were subjected to an electrical stimulation protocol that results in 20 eccentric and 20 concentric contractions during a single session or (n=5; n=4 sham). A separate cohort of mixed-sex mice were subjected to electrical stimulation twice weekly for 4 weeks (n=4; n=4 sham) and 9 weeks (n=3; n=3 sham). At the end of each study, gastrocnemius-soleus complexes were dissected 24h following the final bout of stimulation. Pericyte quantity was assessed by multiplex flow cytometry in all samples. NG2+CD45-CD31- and CD146+CD45-CD31- pericytes were isolated following the acute study and gene expression was evaluated using high throughput qPCR. RESULTS: Acute electrical stimulation resulted in a non-significant trend for an increase in total pericyte content in skeletal muscle and a significant increase in the percentage of NG2+CD45-CD31- pericytes expressing the mesenchymal stem/stromal cell (MSC) marker CD140A. Isolation of pericytes based on CD146 revealed a population of cells highly engaged in the synthesis of factors necessary for myogenesis, satellite cell activation, and extracellular matrix remodeling post-acute stimulation. Finally, a pericyte to MSC transition was also observed with 4 weeks of stimulation, but no changes in overall pericyte quantity were noted at 4 or 9 weeks. CONCLUSION: This study provides evidence that resistance exercise promotes a pericyte to MSC transition, an event that may be necessary for pericytes to engage in skeletal muscle repair and adaptation.","abstract_has_math":false,"creators":["Dvoretskiy, Svyatoslav Victorovich"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Kinesiology","degree_department":null,"school":null,"contributors":["Boppart, Marni D."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-07-07T21:17:38Z","date_published":"2016-07-07T21:17:38Z","updated_at":"2026-07-22T22:26:34Z","subjects":["Pericyte","Electrical Stimulation","Skeletal Muscle"],"languages":["en"],"rights":["Copyright 2016 Svyatoslav Dvoretskiy"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/90920","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Boppart, Marni D."]},{"key":"dc:creator","label":"Author","values":["Dvoretskiy, Svyatoslav Victorovich"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2016-07-07T21:17:38Z","2018-07-08T09:15:23Z","2016-04-18","2016-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Kinesiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Pericyte","Electrical Stimulation","Skeletal Muscle"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2016 Svyatoslav Dvoretskiy"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/90920"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Pericytes are mural cells that are located on the outer surface of blood capillaries where they attach to endothelial cells and regulate vascular function, including dilation and angiogenesis. Recent studies suggest that pericytes in skeletal muscle may contribute to myofiber repair in response to injury. However, the pericyte response to exercise remains largely unexplored. PURPOSE: The purpose of this study was to evaluate pericyte quantity and gene expression in skeletal muscle following electrical stimulation, a method that can be used to simulate resistance exercise in mice. METHODS: Adult wild-type mice were subjected to an electrical stimulation protocol that results in 20 eccentric and 20 concentric contractions during a single session or (n=5; n=4 sham). A separate cohort of mixed-sex mice were subjected to electrical stimulation twice weekly for 4 weeks (n=4; n=4 sham) and 9 weeks (n=3; n=3 sham). At the end of each study, gastrocnemius-soleus complexes were dissected 24h following the final bout of stimulation. Pericyte quantity was assessed by multiplex flow cytometry in all samples. NG2+CD45-CD31- and CD146+CD45-CD31- pericytes were isolated following the acute study and gene expression was evaluated using high throughput qPCR. RESULTS: Acute electrical stimulation resulted in a non-significant trend for an increase in total pericyte content in skeletal muscle and a significant increase in the percentage of NG2+CD45-CD31- pericytes expressing the mesenchymal stem/stromal cell (MSC) marker CD140A. Isolation of pericytes based on CD146 revealed a population of cells highly engaged in the synthesis of factors necessary for myogenesis, satellite cell activation, and extracellular matrix remodeling post-acute stimulation. Finally, a pericyte to MSC transition was also observed with 4 weeks of stimulation, but no changes in overall pericyte quantity were noted at 4 or 9 weeks. CONCLUSION: This study provides evidence that resistance exercise promotes a pericyte to MSC transition, an event that may be necessary for pericytes to engage in skeletal muscle repair and adaptation.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2018-05-01","The student, Svyatoslav Dvoretskiy, accepted the attached license on 2016-04-18 at 10:37.","The student, Svyatoslav Dvoretskiy, submitted this Thesis for approval on 2016-04-18 at 10:45.","This Thesis was approved for publication on 2016-04-18 at 15:04.","DSpace SAF Submission Ingestion Package generated from Vireo submission #9288 on 2016-07-07 at 14:17:06","Made available in DSpace on 2016-07-07T21:17:38Z (GMT). No. of bitstreams: 3 DVORETSKIY-THESIS-2016.pdf: 943636 bytes, checksum: a51d0c01ed22ff6dae6fc103672228c2 (MD5) Svyatoslav Dvoretskiy Master Thesis.docx: 2367043 bytes, checksum: db5486db70b873dbb92345256c1d3644 (MD5) LICENSE.txt: 4218 bytes, checksum: 85ae590de1e348ae15a1c9453a69eb5f (MD5) Previous issue date: 2016-04-18","Embargo set by: Seth Robbins for item 93275 Lift date: 2018-07-07T21:18:16Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 93275 on 2018-07-08T09:15:23Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Skeletal muscle pericyte response to acute and chronic electrical stimulation"]}]}],"canonical_facts":{"dc:contributor":["Boppart, Marni D."],"dc:creator":["Dvoretskiy, Svyatoslav Victorovich"],"dc:date":["2016-07-07T21:17:38Z","2018-07-08T09:15:23Z","2016-04-18","2016-05"],"dc:description":["Pericytes are mural cells that are located on the outer surface of blood capillaries where they attach to endothelial cells and regulate vascular function, including dilation and angiogenesis. Recent studies suggest that pericytes in skeletal muscle may contribute to myofiber repair in response to injury. However, the pericyte response to exercise remains largely unexplored. PURPOSE: The purpose of this study was to evaluate pericyte quantity and gene expression in skeletal muscle following electrical stimulation, a method that can be used to simulate resistance exercise in mice. METHODS: Adult wild-type mice were subjected to an electrical stimulation protocol that results in 20 eccentric and 20 concentric contractions during a single session or (n=5; n=4 sham). A separate cohort of mixed-sex mice were subjected to electrical stimulation twice weekly for 4 weeks (n=4; n=4 sham) and 9 weeks (n=3; n=3 sham). At the end of each study, gastrocnemius-soleus complexes were dissected 24h following the final bout of stimulation. Pericyte quantity was assessed by multiplex flow cytometry in all samples. NG2+CD45-CD31- and CD146+CD45-CD31- pericytes were isolated following the acute study and gene expression was evaluated using high throughput qPCR. RESULTS: Acute electrical stimulation resulted in a non-significant trend for an increase in total pericyte content in skeletal muscle and a significant increase in the percentage of NG2+CD45-CD31- pericytes expressing the mesenchymal stem/stromal cell (MSC) marker CD140A. Isolation of pericytes based on CD146 revealed a population of cells highly engaged in the synthesis of factors necessary for myogenesis, satellite cell activation, and extracellular matrix remodeling post-acute stimulation. Finally, a pericyte to MSC transition was also observed with 4 weeks of stimulation, but no changes in overall pericyte quantity were noted at 4 or 9 weeks. CONCLUSION: This study provides evidence that resistance exercise promotes a pericyte to MSC transition, an event that may be necessary for pericytes to engage in skeletal muscle repair and adaptation.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2018-05-01","The student, Svyatoslav Dvoretskiy, accepted the attached license on 2016-04-18 at 10:37.","The student, Svyatoslav Dvoretskiy, submitted this Thesis for approval on 2016-04-18 at 10:45.","This Thesis was approved for publication on 2016-04-18 at 15:04.","DSpace SAF Submission Ingestion Package generated from Vireo submission #9288 on 2016-07-07 at 14:17:06","Made available in DSpace on 2016-07-07T21:17:38Z (GMT). No. of bitstreams: 3 DVORETSKIY-THESIS-2016.pdf: 943636 bytes, checksum: a51d0c01ed22ff6dae6fc103672228c2 (MD5) Svyatoslav Dvoretskiy Master Thesis.docx: 2367043 bytes, checksum: db5486db70b873dbb92345256c1d3644 (MD5) LICENSE.txt: 4218 bytes, checksum: 85ae590de1e348ae15a1c9453a69eb5f (MD5) Previous issue date: 2016-04-18","Embargo set by: Seth Robbins for item 93275 Lift date: 2018-07-07T21:18:16Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 93275 on 2018-07-08T09:15:23Z."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/90920"],"dc:language":["en"],"dc:rights":["Copyright 2016 Svyatoslav Dvoretskiy"],"dc:subject":["Pericyte","Electrical Stimulation","Skeletal Muscle"],"dc:title":["Skeletal muscle pericyte response to acute and chronic electrical stimulation"],"dc:type":["text"],"thesis:degree_discipline":["Kinesiology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:34Z"}