Dietary tomato carotenoids and β-carotene-15,15'- dioxygenase (Bco1) genotype alter androgen metabolism, lipid metabolism, and prostate cancer progression in mice
type: CreativeWork
description: β-carotene-15,15'-dioxygenase (BCO1) cleaves dietary carotenoids at the central 15,15' double bond, most notably acting on β-carotene to yield retinal. Much work has gone into the description of enzymatic carotenoid cleavage activities of the enzyme, but although pleiotropic effects of Bco1 loss on lipid and cholesterol metabolism in vivo have occasionally been described, few investigators have reported extensively on this. Furthermore, given the intersections of lipid metabolism, cholesterol/steroid metabolism, and carotenoid transport and distribution, functions of BCO1 within these interdependent realms is of clear physiological relevance. Moreover, since observational data in humans have repeatedly demonstrated that tomato carotenoid intake is associated with a reduced risk of primary prostate cancer (PCa), fundamental knowledge of the role of BCO1 on steroid and lipid metabolism is imperative for proper interpretation of epidemiological and experimental data demonstrating a protective role of dietary tomato. Thus, using animal models, we sought to: elucidate the carotenoid cleavageindependent role of Bco1 on circulating testosterone and prostatic androgen signaling; determine the impact of Bco1 on lipid, cholesterol, and retinoid metabolism in the liver, a key organ in the metabolism and biodistribution of dietary carotenoids; and evaluate the effects of dietary tomato feeding in an animal model of PCa and determine its efficacy in preventing disease progression. In Chapter 2, using the Bco1-/- mouse model, we sought to probe the effects of Bco1 ablation on testicular steroidogenesis, serum testosterone levels, and prostatic androgen signaling. Male wild-type (WT) and Bco1-/- mice were raised on carotenoid-free AIN-93G diets before sacrifice between 10-14 weeks of age. We observed a significant reduction in serum testosterone levels due to Bco1-/- genotype, accompanied by significantly reduced weights of the prostate and seminal vesicles. This reduction in seru…
abstract: β-carotene-15,15'-dioxygenase (BCO1) cleaves dietary carotenoids at the central 15,15' double bond, most notably acting on β-carotene to yield retinal. Much work has gone into the description of enzymatic carotenoid cleavage activities of the enzyme, but although pleiotropic effects of Bco1 loss on lipid and cholesterol metabolism in vivo have occasionally been described, few investigators have reported extensively on this. Furthermore, given the intersections of lipid metabolism, cholesterol/steroid metabolism, and carotenoid transport and distribution, functions of BCO1 within these interdependent realms is of clear physiological relevance. Moreover, since observational data in humans have repeatedly demonstrated that tomato carotenoid intake is associated with a reduced risk of primary prostate cancer (PCa), fundamental knowledge of the role of BCO1 on steroid and lipid metabolism is imperative for proper interpretation of epidemiological and experimental data demonstrating a protective role of dietary tomato. Thus, using animal models, we sought to: elucidate the carotenoid cleavageindependent role of Bco1 on circulating testosterone and prostatic androgen signaling; determine the impact of Bco1 on lipid, cholesterol, and retinoid metabolism in the liver, a key organ in the metabolism and biodistribution of dietary carotenoids; and evaluate the effects of dietary tomato feeding in an animal model of PCa and determine its efficacy in preventing disease progression. In Chapter 2, using the Bco1-/- mouse model, we sought to probe the effects of Bco1 ablation on testicular steroidogenesis, serum testosterone levels, and prostatic androgen signaling. Male wild-type (WT) and Bco1-/- mice were raised on carotenoid-free AIN-93G diets before sacrifice between 10-14 weeks of age. We observed a significant reduction in serum testosterone levels due to Bco1-/- genotype, accompanied by significantly reduced weights of the prostate and seminal vesicles. This reduction in seru…
author: Smith, Joshua William
language: en
keywords: tomato, Bacon, prostate cancer, castration-resistant prostate cancer, carotenoids, lipid, retinoid, retinol, retinal ester, liver, hepatic, testosterone, androgen
date published: 2016-07-07T21:17:31Z
date modified: 2026-07-22T22:26:34Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/90908