{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/90566"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/90566","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Secondary anchor targeted cell release system","abstract":"The lack of diagnostic tools that can probe individual heterogeneities in patient’s cell receptor expression limits advancement in personalized medicine. These individual differences in receptor quantities can give rise to both intrinsic and acquired resistances to therapeutics, which result in reduced treatment efficacy. In diseases like cancer, where therapeutics have many adverse side effects, noting which drugs have reduced efficacy means the difference between remission and death. Current chemical and physical cell separation methodologies may result in disruption of physiological receptor quantities. These changes in receptor quantities and expression may hide changes that give diagnostic information about tumor progression and environment. Thus separation techniques which hide these changes in receptor expression would be sub-optimal diagnostic tools. Here we describe a functionalization process that facilitates gentle cell capture with subsequent cell release via a secondary, surface-anchoring mechanism. The cellular capture system consists of glass functionalized with APTES, d-desthiobiotin and streptavidin, which when coupled with biotinylated antibodies, such as mCD11b and hIgG, are used to capture mouse macrophages (RAW 264.7) and human breast cancer (MCF7-GFP) cell lines, respectively. Cell release is facilitated through the introduction of biotin, allowing for the enrichment of the cells of interest captured by the surface. This release is done through the targeting of the secondary moiety desthiobiotin, which results in a much more gentle release paradigm. This reduction in harsh reagents and shear forces reduces changes in cellular expression. The functionalized surface captures up to 80% of cells in a single cell mixture and has demonstrated 50% capture in a dual-cell mixture. This engineering advancement is a critical step towards achieving cell isolation platforms for personalized medicine.","abstract_html":"The lack of diagnostic tools that can probe individual heterogeneities in patient’s cell receptor expression limits advancement in personalized medicine. These individual differences in receptor quantities can give rise to both intrinsic and acquired resistances to therapeutics, which result in reduced treatment efficacy. In diseases like cancer, where therapeutics have many adverse side effects, noting which drugs have reduced efficacy means the difference between remission and death. Current chemical and physical cell separation methodologies may result in disruption of physiological receptor quantities. These changes in receptor quantities and expression may hide changes that give diagnostic information about tumor progression and environment. Thus separation techniques which hide these changes in receptor expression would be sub-optimal diagnostic tools. Here we describe a functionalization process that facilitates gentle cell capture with subsequent cell release via a secondary, surface-anchoring mechanism. The cellular capture system consists of glass functionalized with APTES, d-desthiobiotin and streptavidin, which when coupled with biotinylated antibodies, such as mCD11b and hIgG, are used to capture mouse macrophages (RAW 264.7) and human breast cancer (MCF7-GFP) cell lines, respectively. Cell release is facilitated through the introduction of biotin, allowing for the enrichment of the cells of interest captured by the surface. This release is done through the targeting of the secondary moiety desthiobiotin, which results in a much more gentle release paradigm. This reduction in harsh reagents and shear forces reduces changes in cellular expression. The functionalized surface captures up to 80% of cells in a single cell mixture and has demonstrated 50% capture in a dual-cell mixture. This engineering advancement is a critical step towards achieving cell isolation platforms for personalized medicine.","abstract_has_math":false,"creators":["Ansari, Ali"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Imoukhuede, Princess I."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-07-07T19:54:10Z","date_published":"2016-07-07T19:54:10Z","updated_at":"2026-07-22T22:26:32Z","subjects":["Surface Functionalization","MCF7gfp","RAW 264.7","Cell Isolation"],"languages":["en"],"rights":["Copyright 2016 Ali Ansari"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/90566","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Imoukhuede, Princess I."]},{"key":"dc:creator","label":"Author","values":["Ansari, Ali"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2016-07-07T19:54:10Z","2016-04-21","2016-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Surface Functionalization","MCF7gfp","RAW 264.7","Cell Isolation"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2016 Ali Ansari"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/90566"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The lack of diagnostic tools that can probe individual heterogeneities in patient’s cell receptor expression limits advancement in personalized medicine. These individual differences in receptor quantities can give rise to both intrinsic and acquired resistances to therapeutics, which result in reduced treatment efficacy. In diseases like cancer, where therapeutics have many adverse side effects, noting which drugs have reduced efficacy means the difference between remission and death. Current chemical and physical cell separation methodologies may result in disruption of physiological receptor quantities. These changes in receptor quantities and expression may hide changes that give diagnostic information about tumor progression and environment. Thus separation techniques which hide these changes in receptor expression would be sub-optimal diagnostic tools. Here we describe a functionalization process that facilitates gentle cell capture with subsequent cell release via a secondary, surface-anchoring mechanism. The cellular capture system consists of glass functionalized with APTES, d-desthiobiotin and streptavidin, which when coupled with biotinylated antibodies, such as mCD11b and hIgG, are used to capture mouse macrophages (RAW 264.7) and human breast cancer (MCF7-GFP) cell lines, respectively. Cell release is facilitated through the introduction of biotin, allowing for the enrichment of the cells of interest captured by the surface. This release is done through the targeting of the secondary moiety desthiobiotin, which results in a much more gentle release paradigm. This reduction in harsh reagents and shear forces reduces changes in cellular expression. The functionalized surface captures up to 80% of cells in a single cell mixture and has demonstrated 50% capture in a dual-cell mixture. This engineering advancement is a critical step towards achieving cell isolation platforms for personalized medicine.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2016-07-07 without embargo terms","The student, Ali Ansari, accepted the attached license on 2016-04-18 at 16:40.","The student, Ali Ansari, submitted this Thesis for approval on 2016-04-19 at 12:21.","This Thesis was approved for publication on 2016-04-21 at 10:14.","DSpace SAF Submission Ingestion Package generated from Vireo submission #9314 on 2016-07-07 at 13:31:15","Made available in DSpace on 2016-07-07T19:54:10Z (GMT). No. of bitstreams: 3 ANSARI-THESIS-2016.pdf: 1818449 bytes, checksum: aeaddb5f7f290bfd11d3953f5ae3b5b4 (MD5) LICENSE.txt: 4207 bytes, checksum: 3e8f6fe704fe867c59b3a8f54a3fbe1e (MD5) WileyPermission.pdf: 109926 bytes, checksum: 18701ab4e634997b877cecc4568e3e7c (MD5) Previous issue date: 2016-04-21"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Secondary anchor targeted cell release system"]}]}],"canonical_facts":{"dc:contributor":["Imoukhuede, Princess I."],"dc:creator":["Ansari, Ali"],"dc:date":["2016-07-07T19:54:10Z","2016-04-21","2016-05"],"dc:description":["The lack of diagnostic tools that can probe individual heterogeneities in patient’s cell receptor expression limits advancement in personalized medicine. These individual differences in receptor quantities can give rise to both intrinsic and acquired resistances to therapeutics, which result in reduced treatment efficacy. In diseases like cancer, where therapeutics have many adverse side effects, noting which drugs have reduced efficacy means the difference between remission and death. Current chemical and physical cell separation methodologies may result in disruption of physiological receptor quantities. These changes in receptor quantities and expression may hide changes that give diagnostic information about tumor progression and environment. Thus separation techniques which hide these changes in receptor expression would be sub-optimal diagnostic tools. Here we describe a functionalization process that facilitates gentle cell capture with subsequent cell release via a secondary, surface-anchoring mechanism. The cellular capture system consists of glass functionalized with APTES, d-desthiobiotin and streptavidin, which when coupled with biotinylated antibodies, such as mCD11b and hIgG, are used to capture mouse macrophages (RAW 264.7) and human breast cancer (MCF7-GFP) cell lines, respectively. Cell release is facilitated through the introduction of biotin, allowing for the enrichment of the cells of interest captured by the surface. This release is done through the targeting of the secondary moiety desthiobiotin, which results in a much more gentle release paradigm. This reduction in harsh reagents and shear forces reduces changes in cellular expression. The functionalized surface captures up to 80% of cells in a single cell mixture and has demonstrated 50% capture in a dual-cell mixture. This engineering advancement is a critical step towards achieving cell isolation platforms for personalized medicine.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2016-07-07 without embargo terms","The student, Ali Ansari, accepted the attached license on 2016-04-18 at 16:40.","The student, Ali Ansari, submitted this Thesis for approval on 2016-04-19 at 12:21.","This Thesis was approved for publication on 2016-04-21 at 10:14.","DSpace SAF Submission Ingestion Package generated from Vireo submission #9314 on 2016-07-07 at 13:31:15","Made available in DSpace on 2016-07-07T19:54:10Z (GMT). No. of bitstreams: 3 ANSARI-THESIS-2016.pdf: 1818449 bytes, checksum: aeaddb5f7f290bfd11d3953f5ae3b5b4 (MD5) LICENSE.txt: 4207 bytes, checksum: 3e8f6fe704fe867c59b3a8f54a3fbe1e (MD5) WileyPermission.pdf: 109926 bytes, checksum: 18701ab4e634997b877cecc4568e3e7c (MD5) Previous issue date: 2016-04-21"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/90566"],"dc:language":["en"],"dc:rights":["Copyright 2016 Ali Ansari"],"dc:subject":["Surface Functionalization","MCF7gfp","RAW 264.7","Cell Isolation"],"dc:title":["Secondary anchor targeted cell release system"],"dc:type":["text"],"thesis:degree_discipline":["Bioengineering"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:32Z"}