{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/90452"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/90452","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Raman spectroscopic analysis of fatty acids in tumor micro- and macroenvironments in breast cancer","abstract":"Fatty acids play essential roles in the growth and metastasis of cancer cells. To facilitate their avid growth and proliferation, cancer cells not only alter the fatty acid synthesis and metabolism intracellularly and extracellularly, but also in the macroenvironment via direct and indirect pathways. This thesis reports that by using Raman micro-spectroscopy, an increase in the production of polyunsaturated fatty acids (PUFAs) was identified in both cancerous and normal appearing breast tissue obtained from breast cancer patients and tumor-bearing rats. By minimizing confounding effects from mixed chemicals and optimizing the signal-to-noise ratio of Raman spectra, a large-scale transition from monounsaturated fatty acids to PUFAs was observed in the tumor while only a small subset of fatty acids transitioned to PUFAs in the tumor micro- and macroenvironment. These findings demonstrate the power of this spectroscopic analysis, and may provide new insights into the macroenvironmental regulations in breast cancer.","abstract_html":"Fatty acids play essential roles in the growth and metastasis of cancer cells. To facilitate their avid growth and proliferation, cancer cells not only alter the fatty acid synthesis and metabolism intracellularly and extracellularly, but also in the macroenvironment via direct and indirect pathways. This thesis reports that by using Raman micro-spectroscopy, an increase in the production of polyunsaturated fatty acids (PUFAs) was identified in both cancerous and normal appearing breast tissue obtained from breast cancer patients and tumor-bearing rats. By minimizing confounding effects from mixed chemicals and optimizing the signal-to-noise ratio of Raman spectra, a large-scale transition from monounsaturated fatty acids to PUFAs was observed in the tumor while only a small subset of fatty acids transitioned to PUFAs in the tumor micro- and macroenvironment. These findings demonstrate the power of this spectroscopic analysis, and may provide new insights into the macroenvironmental regulations in breast cancer.","abstract_has_math":false,"creators":["You, Sixian"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Boppart, Stephen A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-07-07T19:52:31Z","date_published":"2016-07-07T19:52:31Z","updated_at":"2026-07-22T22:26:32Z","subjects":["Raman spectroscopy","Breast cancer"],"languages":["en"],"rights":["Copy right 2015 Sixian You"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/90452","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Boppart, Stephen A."]},{"key":"dc:creator","label":"Author","values":["You, Sixian"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2016-07-07T19:52:31Z","2015-12-07","2016-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Raman spectroscopy","Breast cancer"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copy right 2015 Sixian You"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/90452"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Fatty acids play essential roles in the growth and metastasis of cancer cells. To facilitate their avid growth and proliferation, cancer cells not only alter the fatty acid synthesis and metabolism intracellularly and extracellularly, but also in the macroenvironment via direct and indirect pathways. This thesis reports that by using Raman micro-spectroscopy, an increase in the production of polyunsaturated fatty acids (PUFAs) was identified in both cancerous and normal appearing breast tissue obtained from breast cancer patients and tumor-bearing rats. By minimizing confounding effects from mixed chemicals and optimizing the signal-to-noise ratio of Raman spectra, a large-scale transition from monounsaturated fatty acids to PUFAs was observed in the tumor while only a small subset of fatty acids transitioned to PUFAs in the tumor micro- and macroenvironment. These findings demonstrate the power of this spectroscopic analysis, and may provide new insights into the macroenvironmental regulations in breast cancer.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2016-07-07 without embargo terms","The student, Sixian You, accepted the attached license on 2015-12-02 at 13:54.","The student, Sixian You, submitted this Thesis for approval on 2015-12-02 at 14:00.","This Thesis was approved for publication on 2015-12-07 at 16:35.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8905 on 2016-07-07 at 13:26:32","Made available in DSpace on 2016-07-07T19:52:31Z (GMT). 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This thesis reports that by using Raman micro-spectroscopy, an increase in the production of polyunsaturated fatty acids (PUFAs) was identified in both cancerous and normal appearing breast tissue obtained from breast cancer patients and tumor-bearing rats. By minimizing confounding effects from mixed chemicals and optimizing the signal-to-noise ratio of Raman spectra, a large-scale transition from monounsaturated fatty acids to PUFAs was observed in the tumor while only a small subset of fatty acids transitioned to PUFAs in the tumor micro- and macroenvironment. These findings demonstrate the power of this spectroscopic analysis, and may provide new insights into the macroenvironmental regulations in breast cancer.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2016-07-07 without embargo terms","The student, Sixian You, accepted the attached license on 2015-12-02 at 13:54.","The student, Sixian You, submitted this Thesis for approval on 2015-12-02 at 14:00.","This Thesis was approved for publication on 2015-12-07 at 16:35.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8905 on 2016-07-07 at 13:26:32","Made available in DSpace on 2016-07-07T19:52:31Z (GMT). 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