{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/89219"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/89219","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Studies on the interaction between porcine reproductive and respiratory syndrome virus and its natural host cell","abstract":"Porcine reproductive and respiratory syndrome virus (PRRSV), an arterivirus, causes a costly global disease of swine. PRRSV infects their alveolar macrophages (AMΦ) resulting in an interstitial pneumonia. The ability of PRRSV to modulate the production of interferon (IFN)a and tumor necrosis factor (TNF)a by affected AMΦ is implicated in the virus’s pathogenesis. In this regard, infection of porcine AMΦ with PRRSV reduced by >50% the amount of IFN-α otherwise produced following the cells’ exposure to synthetic dsRNA. Interestingly, there was no corresponding impairment of the activation of either interferon regulatory factor 3 (IRF3) or signal transducer and activator of transcription 1 (STAT1), or of the transcription of the IFN-α, IFN-b, or IRF7 genes. Rather, the reduction correlated with the phosphorylation of eukaryotic translation initiation factor 2 (eIF2)a via the protein kinase RNA-like endoplasmic reticulum (ER) kinase (PERK) and the appearance of stress granules, indicating translational attenuation. Likewise, a TNF-α response to lipopolysaccharide (LPS) was inhibited when this toll-like receptor (TLR)4 agonist was introduced at 6 h post-infection, when peak TNF-α synthesis and eIF2α phosphorylation would coincide. In contrast, a synergistic TNFα response, due to NFkB activation via the inositol-requiring protein-1α (IRE-1α), was observed if LPS exposure occurred 4 h earlier enabling subsequent TNFα production to be unaffected by later eIF2α phosphorylation. Thus, representatives of two branches of the unfolded protein response (UPR) of AMΦ to PRRSV replication, IRE-1α and PERK, can enhance or suppress cytokine production by triggering the activation of NF-κB or eIF2a, respectively. It worth noting that the modulatory activities became critical when the PRRSV-infected cells were super-exposed to secondary stimulation of poly(I:C) or LPS. In which case exacerbated inflammation response in the lung will develop and produce significant morbidity and death. In the case of PRRSV infection alone, these cytokine responses such IFN-α are barely detected in spite of massive dsRNA produced in PRRSV-infected porcine AMΦ cells. As dsRNA is currently considered to be the most potent type I interferon (IFN) agonist, it has been suggested that sequestration of viral dsRNA may also help viruses evade host innate immune detection by reducing exposure of viral dsRNA to viral nucleic acids cytoplasmic sensors. This predicted type of immune evasion by virus-induced intracellular membrane structures had been confirmed for flaviviruses. By coupling a selective permeablization technique with immunostaining analysis, a positive correlation between the cytosolic exposure of virus dsRNA and a host type I IFN response was demonstrated. In this case, the extent of flavivirus dsRNA exposure was dependent on both the virus species and host cell type. Despite lacking definitive experimental evidence regarding members of the nidovirales family including arteriviruses and coronaviruses, it has been suggested that these viruses also utilize a similar immune evasion strategy. In this regard, the development of double membrane vesicle (DMV) structures has been associated with the activity of arterivirus nsp2 and its coronavirus equivalent, nsp3. Moreover, in our previous study, the arterivirus, porcine reproductive and respiratory syndrome virus (PRRSV) was shown to be incapable of inducing type I IFN synthesis upon infecting porcine alveolar macrophages. Thus, PRRSV appears to utilize a passive innate immunity evading mechanism. In the present study, in conjunction with the application of selective permeabilization, our immuno-staining results demonstrated for the first time that during PRRSV infection the viral dsRNA is sequestered inside intracellular membranes, which could prevent detection of virus dsRNA by host’s viral nucleic acid sensor. Evidence that it is indeed the case was provided by a laboratory PRRSV strain with a unique and deleterious mutation in the N-terminal region of NSP2, for which the virus losses the ability to sequester its dsRNA inside intracellular membrane structures and thus away from detection by viral nucleic acid sensors, resulting in a substantial type I IFN response upon infecting alveolar macrophages.","abstract_html":"Porcine reproductive and respiratory syndrome virus (PRRSV), an arterivirus, causes a costly global disease of swine. PRRSV infects their alveolar macrophages (AMΦ) resulting in an interstitial pneumonia. The ability of PRRSV to modulate the production of interferon (IFN)a and tumor necrosis factor (TNF)a by affected AMΦ is implicated in the virus’s pathogenesis. In this regard, infection of porcine AMΦ with PRRSV reduced by >50% the amount of IFN-α otherwise produced following the cells’ exposure to synthetic dsRNA. Interestingly, there was no corresponding impairment of the activation of either interferon regulatory factor 3 (IRF3) or signal transducer and activator of transcription 1 (STAT1), or of the transcription of the IFN-α, IFN-b, or IRF7 genes. Rather, the reduction correlated with the phosphorylation of eukaryotic translation initiation factor 2 (eIF2)a via the protein kinase RNA-like endoplasmic reticulum (ER) kinase (PERK) and the appearance of stress granules, indicating translational attenuation. Likewise, a TNF-α response to lipopolysaccharide (LPS) was inhibited when this toll-like receptor (TLR)4 agonist was introduced at 6 h post-infection, when peak TNF-α synthesis and eIF2α phosphorylation would coincide. In contrast, a synergistic TNFα response, due to NFkB activation via the inositol-requiring protein-1α (IRE-1α), was observed if LPS exposure occurred 4 h earlier enabling subsequent TNFα production to be unaffected by later eIF2α phosphorylation. Thus, representatives of two branches of the unfolded protein response (UPR) of AMΦ to PRRSV replication, IRE-1α and PERK, can enhance or suppress cytokine production by triggering the activation of NF-κB or eIF2a, respectively. It worth noting that the modulatory activities became critical when the PRRSV-infected cells were super-exposed to secondary stimulation of poly(I:C) or LPS. In which case exacerbated inflammation response in the lung will develop and produce significant morbidity and death. In the case of PRRSV infection alone, these cytokine responses such IFN-α are barely detected in spite of massive dsRNA produced in PRRSV-infected porcine AMΦ cells. As dsRNA is currently considered to be the most potent type I interferon (IFN) agonist, it has been suggested that sequestration of viral dsRNA may also help viruses evade host innate immune detection by reducing exposure of viral dsRNA to viral nucleic acids cytoplasmic sensors. This predicted type of immune evasion by virus-induced intracellular membrane structures had been confirmed for flaviviruses. By coupling a selective permeablization technique with immunostaining analysis, a positive correlation between the cytosolic exposure of virus dsRNA and a host type I IFN response was demonstrated. In this case, the extent of flavivirus dsRNA exposure was dependent on both the virus species and host cell type. Despite lacking definitive experimental evidence regarding members of the nidovirales family including arteriviruses and coronaviruses, it has been suggested that these viruses also utilize a similar immune evasion strategy. In this regard, the development of double membrane vesicle (DMV) structures has been associated with the activity of arterivirus nsp2 and its coronavirus equivalent, nsp3. Moreover, in our previous study, the arterivirus, porcine reproductive and respiratory syndrome virus (PRRSV) was shown to be incapable of inducing type I IFN synthesis upon infecting porcine alveolar macrophages. Thus, PRRSV appears to utilize a passive innate immunity evading mechanism. In the present study, in conjunction with the application of selective permeabilization, our immuno-staining results demonstrated for the first time that during PRRSV infection the viral dsRNA is sequestered inside intracellular membranes, which could prevent detection of virus dsRNA by host’s viral nucleic acid sensor. Evidence that it is indeed the case was provided by a laboratory PRRSV strain with a unique and deleterious mutation in the N-terminal region of NSP2, for which the virus losses the ability to sequester its dsRNA inside intracellular membrane structures and thus away from detection by viral nucleic acid sensors, resulting in a substantial type I IFN response upon infecting alveolar macrophages.","abstract_has_math":false,"creators":["Chen, Wei Yu"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"VMS - Veterinary Pathobiology","degree_department":null,"school":null,"contributors":["Zuckermann, Federico","Rock, Daniel","Segre, Mariangela","Shisler, Joanna"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-03-02T21:06:52Z","date_published":"2016-03-02T21:06:52Z","updated_at":"2026-07-22T22:26:32Z","subjects":["Alveolar Macrophages","Innate immunity","endoplasmic reticulum","Unfolded Protein Response","IRE1","eIF2","type I interferon","tumor necrosis factor alpha (TNFa)","double stranded ribonucleic acid (dsRNA)"],"languages":["en"],"rights":["Copyright 2015 Wei Yu Chen"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/89219","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Zuckermann, Federico","Rock, Daniel","Segre, Mariangela","Shisler, Joanna"]},{"key":"dc:creator","label":"Author","values":["Chen, Wei Yu"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2016-03-02T21:06:52Z","2018-03-03T10:15:28Z","2015-12-01","2015-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["VMS - Veterinary Pathobiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Alveolar Macrophages","Innate immunity","endoplasmic reticulum","Unfolded Protein Response","IRE1","eIF2","type I interferon","tumor necrosis factor alpha (TNFa)","double stranded ribonucleic acid (dsRNA)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2015 Wei Yu Chen"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/89219"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Porcine reproductive and respiratory syndrome virus (PRRSV), an arterivirus, causes a costly global disease of swine. PRRSV infects their alveolar macrophages (AMΦ) resulting in an interstitial pneumonia. The ability of PRRSV to modulate the production of interferon (IFN)a and tumor necrosis factor (TNF)a by affected AMΦ is implicated in the virus’s pathogenesis. In this regard, infection of porcine AMΦ with PRRSV reduced by >50% the amount of IFN-α otherwise produced following the cells’ exposure to synthetic dsRNA. Interestingly, there was no corresponding impairment of the activation of either interferon regulatory factor 3 (IRF3) or signal transducer and activator of transcription 1 (STAT1), or of the transcription of the IFN-α, IFN-b, or IRF7 genes. Rather, the reduction correlated with the phosphorylation of eukaryotic translation initiation factor 2 (eIF2)a via the protein kinase RNA-like endoplasmic reticulum (ER) kinase (PERK) and the appearance of stress granules, indicating translational attenuation. Likewise, a TNF-α response to lipopolysaccharide (LPS) was inhibited when this toll-like receptor (TLR)4 agonist was introduced at 6 h post-infection, when peak TNF-α synthesis and eIF2α phosphorylation would coincide. In contrast, a synergistic TNFα response, due to NFkB activation via the inositol-requiring protein-1α (IRE-1α), was observed if LPS exposure occurred 4 h earlier enabling subsequent TNFα production to be unaffected by later eIF2α phosphorylation. Thus, representatives of two branches of the unfolded protein response (UPR) of AMΦ to PRRSV replication, IRE-1α and PERK, can enhance or suppress cytokine production by triggering the activation of NF-κB or eIF2a, respectively. It worth noting that the modulatory activities became critical when the PRRSV-infected cells were super-exposed to secondary stimulation of poly(I:C) or LPS. In which case exacerbated inflammation response in the lung will develop and produce significant morbidity and death. In the case of PRRSV infection alone, these cytokine responses such IFN-α are barely detected in spite of massive dsRNA produced in PRRSV-infected porcine AMΦ cells. As dsRNA is currently considered to be the most potent type I interferon (IFN) agonist, it has been suggested that sequestration of viral dsRNA may also help viruses evade host innate immune detection by reducing exposure of viral dsRNA to viral nucleic acids cytoplasmic sensors. This predicted type of immune evasion by virus-induced intracellular membrane structures had been confirmed for flaviviruses. By coupling a selective permeablization technique with immunostaining analysis, a positive correlation between the cytosolic exposure of virus dsRNA and a host type I IFN response was demonstrated. In this case, the extent of flavivirus dsRNA exposure was dependent on both the virus species and host cell type. Despite lacking definitive experimental evidence regarding members of the nidovirales family including arteriviruses and coronaviruses, it has been suggested that these viruses also utilize a similar immune evasion strategy. In this regard, the development of double membrane vesicle (DMV) structures has been associated with the activity of arterivirus nsp2 and its coronavirus equivalent, nsp3. Moreover, in our previous study, the arterivirus, porcine reproductive and respiratory syndrome virus (PRRSV) was shown to be incapable of inducing type I IFN synthesis upon infecting porcine alveolar macrophages. Thus, PRRSV appears to utilize a passive innate immunity evading mechanism. In the present study, in conjunction with the application of selective permeabilization, our immuno-staining results demonstrated for the first time that during PRRSV infection the viral dsRNA is sequestered inside intracellular membranes, which could prevent detection of virus dsRNA by host’s viral nucleic acid sensor. Evidence that it is indeed the case was provided by a laboratory PRRSV strain with a unique and deleterious mutation in the N-terminal region of NSP2, for which the virus losses the ability to sequester its dsRNA inside intracellular membrane structures and thus away from detection by viral nucleic acid sensors, resulting in a substantial type I IFN response upon infecting alveolar macrophages.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2017-12-01","The student, Wei Yu Chen, accepted the attached license on 2015-12-01 at 10:42.","The student, Wei Yu Chen, submitted this Dissertation for approval on 2015-12-01 at 10:55.","This Dissertation was approved for publication on 2015-12-01 at 16:11.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8889 on 2016-03-02 at 14:13:40","Made available in DSpace on 2016-03-02T21:06:52Z (GMT). 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PRRSV infects their alveolar macrophages (AMΦ) resulting in an interstitial pneumonia. The ability of PRRSV to modulate the production of interferon (IFN)a and tumor necrosis factor (TNF)a by affected AMΦ is implicated in the virus’s pathogenesis. In this regard, infection of porcine AMΦ with PRRSV reduced by >50% the amount of IFN-α otherwise produced following the cells’ exposure to synthetic dsRNA. Interestingly, there was no corresponding impairment of the activation of either interferon regulatory factor 3 (IRF3) or signal transducer and activator of transcription 1 (STAT1), or of the transcription of the IFN-α, IFN-b, or IRF7 genes. Rather, the reduction correlated with the phosphorylation of eukaryotic translation initiation factor 2 (eIF2)a via the protein kinase RNA-like endoplasmic reticulum (ER) kinase (PERK) and the appearance of stress granules, indicating translational attenuation. Likewise, a TNF-α response to lipopolysaccharide (LPS) was inhibited when this toll-like receptor (TLR)4 agonist was introduced at 6 h post-infection, when peak TNF-α synthesis and eIF2α phosphorylation would coincide. In contrast, a synergistic TNFα response, due to NFkB activation via the inositol-requiring protein-1α (IRE-1α), was observed if LPS exposure occurred 4 h earlier enabling subsequent TNFα production to be unaffected by later eIF2α phosphorylation. Thus, representatives of two branches of the unfolded protein response (UPR) of AMΦ to PRRSV replication, IRE-1α and PERK, can enhance or suppress cytokine production by triggering the activation of NF-κB or eIF2a, respectively. It worth noting that the modulatory activities became critical when the PRRSV-infected cells were super-exposed to secondary stimulation of poly(I:C) or LPS. In which case exacerbated inflammation response in the lung will develop and produce significant morbidity and death. In the case of PRRSV infection alone, these cytokine responses such IFN-α are barely detected in spite of massive dsRNA produced in PRRSV-infected porcine AMΦ cells. As dsRNA is currently considered to be the most potent type I interferon (IFN) agonist, it has been suggested that sequestration of viral dsRNA may also help viruses evade host innate immune detection by reducing exposure of viral dsRNA to viral nucleic acids cytoplasmic sensors. This predicted type of immune evasion by virus-induced intracellular membrane structures had been confirmed for flaviviruses. By coupling a selective permeablization technique with immunostaining analysis, a positive correlation between the cytosolic exposure of virus dsRNA and a host type I IFN response was demonstrated. In this case, the extent of flavivirus dsRNA exposure was dependent on both the virus species and host cell type. Despite lacking definitive experimental evidence regarding members of the nidovirales family including arteriviruses and coronaviruses, it has been suggested that these viruses also utilize a similar immune evasion strategy. In this regard, the development of double membrane vesicle (DMV) structures has been associated with the activity of arterivirus nsp2 and its coronavirus equivalent, nsp3. Moreover, in our previous study, the arterivirus, porcine reproductive and respiratory syndrome virus (PRRSV) was shown to be incapable of inducing type I IFN synthesis upon infecting porcine alveolar macrophages. Thus, PRRSV appears to utilize a passive innate immunity evading mechanism. In the present study, in conjunction with the application of selective permeabilization, our immuno-staining results demonstrated for the first time that during PRRSV infection the viral dsRNA is sequestered inside intracellular membranes, which could prevent detection of virus dsRNA by host’s viral nucleic acid sensor. Evidence that it is indeed the case was provided by a laboratory PRRSV strain with a unique and deleterious mutation in the N-terminal region of NSP2, for which the virus losses the ability to sequester its dsRNA inside intracellular membrane structures and thus away from detection by viral nucleic acid sensors, resulting in a substantial type I IFN response upon infecting alveolar macrophages.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2017-12-01","The student, Wei Yu Chen, accepted the attached license on 2015-12-01 at 10:42.","The student, Wei Yu Chen, submitted this Dissertation for approval on 2015-12-01 at 10:55.","This Dissertation was approved for publication on 2015-12-01 at 16:11.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8889 on 2016-03-02 at 14:13:40","Made available in DSpace on 2016-03-02T21:06:52Z (GMT). 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