{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/89145"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/89145","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Variation and quantitative trait loci analysis of myrosinase activity and phenolic compound accumulation in Brassica oleracea var. italica","abstract":"Made available in DSpace on 2016-03-02T20:24:12Z (GMT). No. of bitstreams: 2 GARDNER-THESIS-2015.pdf: 3191246 bytes, checksum: 282a4f72b0a6003e1ab58dc6ded758c2 (MD5) LICENSE.txt: 4211 bytes, checksum: 9b8349680069b41d9e16d0ea2a47d366 (MD5) Previous issue date: 2015-12-04","abstract_html":"Made available in DSpace on 2016-03-02T20:24:12Z (GMT). No. of bitstreams: 2 GARDNER-THESIS-2015.pdf: 3191246 bytes, checksum: 282a4f72b0a6003e1ab58dc6ded758c2 (MD5) LICENSE.txt: 4211 bytes, checksum: 9b8349680069b41d9e16d0ea2a47d366 (MD5) Previous issue date: 2015-12-04","abstract_has_math":false,"creators":["Gardner, Alicia Marie"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Crop Sciences","degree_department":null,"school":null,"contributors":["Juvik, John A.","Zhao, Youfu"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-03-02T20:24:12Z","date_published":"2016-03-02T20:24:12Z","updated_at":"2026-07-22T22:26:32Z","subjects":["Brassica","Myrosinase","Phenolic compounds","Quantitative trait locus (QTL) mapping"],"languages":["en"],"rights":["Copyright 2015 Alicia Gardner"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/89145","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Juvik, John A.","Zhao, Youfu"]},{"key":"dc:creator","label":"Author","values":["Gardner, Alicia Marie"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2016-03-02T20:24:12Z","2018-03-03T10:15:27Z","2015-12-04","2015-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Brassica","Myrosinase","Phenolic compounds","Quantitative trait locus (QTL) mapping"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2015 Alicia Gardner"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/89145"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Made available in DSpace on 2016-03-02T20:24:12Z (GMT). No. of bitstreams: 2 GARDNER-THESIS-2015.pdf: 3191246 bytes, checksum: 282a4f72b0a6003e1ab58dc6ded758c2 (MD5) LICENSE.txt: 4211 bytes, checksum: 9b8349680069b41d9e16d0ea2a47d366 (MD5) Previous issue date: 2015-12-04","Two classes of secondary metabolites found in Brassica crops are of particular importance for eliciting human health benefits: phenolic compounds and glucosinolate hydrolysis products. These compounds have been demonstrated—among other things—to induce detoxification enzymes, mitigate inflammation, lower the risk of type II diabetes, and decrease cancer risk. In order to better utilize plants for the promotion of human health, a coordinated effort of advancement is needed in all related fields, including the genetic and environmental regulation of plant secondary product biosynthesis and the in vivo targets and mechanisms of action of phytochemicals in humans. This research addresses the genetic control of glucosinolate metabolism and phenolic compound accumulation in broccoli (Brassica oleracea L. var. italica). Gas chromatography was utilized to quantify glucosinolate hydrolysis products in the broccoli mapping population VI-158 × BNC. The same population was also evaluated for phenolic compound accumulation with three chemical assays: total phenolic content, ABTS radical scavenging capacity, and DPPH radical scavenging capacity. Quantitative trait loci analysis was employed for each of these phenotypes to identify genetic loci associated with variation in glucosinolate hydrolysis and phenolic compound accumulation. The genetic linkage map used for this analysis was saturated with single nucleotide polymorphism (SNP) markers anchored to the B. oleracea reference genome TO1000 (Brown et al. 2014). Physical markers were utilized to identify putative candidate genes underlying the QTL effects. This works reveals several questions for further investigation and the potential challenge of improving metabolites that are responsive to environmental conditions, but also highlights potential target genes for breeding Brassica cultivars with greater health-promoting potential.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2017-12-01","The student, Alicia Gardner, accepted the attached license on 2015-12-03 at 11:41.","The student, Alicia Gardner, submitted this Thesis for approval on 2015-12-03 at 11:48.","This Thesis was approved for publication on 2015-12-04 at 15:00.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8918 on 2016-03-02 at 14:07:45","Embargo set by: Seth Robbins for item 91347 Lift date: 2018-03-02T20:24:31Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only Restriction Lifted for Item 91347 on 2018-03-03T10:15:27Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Variation and quantitative trait loci analysis of myrosinase activity and phenolic compound accumulation in Brassica oleracea var. italica"]}]}],"canonical_facts":{"dc:contributor":["Juvik, John A.","Zhao, Youfu"],"dc:creator":["Gardner, Alicia Marie"],"dc:date":["2016-03-02T20:24:12Z","2018-03-03T10:15:27Z","2015-12-04","2015-12"],"dc:description":["Made available in DSpace on 2016-03-02T20:24:12Z (GMT). No. of bitstreams: 2 GARDNER-THESIS-2015.pdf: 3191246 bytes, checksum: 282a4f72b0a6003e1ab58dc6ded758c2 (MD5) LICENSE.txt: 4211 bytes, checksum: 9b8349680069b41d9e16d0ea2a47d366 (MD5) Previous issue date: 2015-12-04","Two classes of secondary metabolites found in Brassica crops are of particular importance for eliciting human health benefits: phenolic compounds and glucosinolate hydrolysis products. These compounds have been demonstrated—among other things—to induce detoxification enzymes, mitigate inflammation, lower the risk of type II diabetes, and decrease cancer risk. In order to better utilize plants for the promotion of human health, a coordinated effort of advancement is needed in all related fields, including the genetic and environmental regulation of plant secondary product biosynthesis and the in vivo targets and mechanisms of action of phytochemicals in humans. This research addresses the genetic control of glucosinolate metabolism and phenolic compound accumulation in broccoli (Brassica oleracea L. var. italica). Gas chromatography was utilized to quantify glucosinolate hydrolysis products in the broccoli mapping population VI-158 × BNC. The same population was also evaluated for phenolic compound accumulation with three chemical assays: total phenolic content, ABTS radical scavenging capacity, and DPPH radical scavenging capacity. Quantitative trait loci analysis was employed for each of these phenotypes to identify genetic loci associated with variation in glucosinolate hydrolysis and phenolic compound accumulation. The genetic linkage map used for this analysis was saturated with single nucleotide polymorphism (SNP) markers anchored to the B. oleracea reference genome TO1000 (Brown et al. 2014). Physical markers were utilized to identify putative candidate genes underlying the QTL effects. This works reveals several questions for further investigation and the potential challenge of improving metabolites that are responsive to environmental conditions, but also highlights potential target genes for breeding Brassica cultivars with greater health-promoting potential.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2017-12-01","The student, Alicia Gardner, accepted the attached license on 2015-12-03 at 11:41.","The student, Alicia Gardner, submitted this Thesis for approval on 2015-12-03 at 11:48.","This Thesis was approved for publication on 2015-12-04 at 15:00.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8918 on 2016-03-02 at 14:07:45","Embargo set by: Seth Robbins for item 91347 Lift date: 2018-03-02T20:24:31Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only Restriction Lifted for Item 91347 on 2018-03-03T10:15:27Z."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/89145"],"dc:language":["en"],"dc:rights":["Copyright 2015 Alicia Gardner"],"dc:subject":["Brassica","Myrosinase","Phenolic compounds","Quantitative trait locus (QTL) mapping"],"dc:title":["Variation and quantitative trait loci analysis of myrosinase activity and phenolic compound accumulation in Brassica oleracea var. italica"],"dc:type":["text"],"thesis:degree_discipline":["Crop Sciences"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:32Z"}