{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/89091"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/89091","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Micron-bubble streaming flow towards micron to sub-micron sized sorting","abstract":"Micro-bubble streaming flows represent a unique type of actuating mechanism for microfluidics and have demonstrated great potential in the applications of micro-particle manipulation (e.g. size dependent trapping, sorting, and focusing etc.). The main object of this thesis work is to extend towards much smaller sized particle sorting down to 1μm and sub-micron. On top of that, we want to further explore the potential of applying bubble streaming sorting to biological objects which in the similar size scales. Systematical experiments have been conducted on polystyrene latex particles as well as several types biological cells and its infectious viruses, specifically Sulfolobus islandicus with Sulfolobus spindle-shaped viruses, and also Escherichia coli with Lambda phage. Size sensitive sorting has been revealed by means of larger sized objects will be majorly deflected away from their original streamline while smaller sized objects will follow, which leads to size-sensitive sorting. It has been proved that relative concentration ratio of two sized particle mixing solution can be enhanced with almost a factor of 6, and can be further improved by sequential bubble sorting. However, sorting application with smaller particles with diameter as 1μm and 100nm doesn’t perform as well as larger particles sorting reported by Wang et al. (Appl. Phys. Lett. 99, 034101, 2011) when they used a mixture of 10μm and 5μm particles in diameter. Fundamental reason of this can be explained by a combination of advection and diffusion effect. Beyond that, the principle of micro-bubble streaming sorting also has shown applicable to biological objects sorting purpose, but could be affected more by (i) wider size distribution, (ii) naturally morphologically diverse, (iii) active motion of live cells and (iv) more deformable compared to artificial polystyrene particles.","abstract_html":"Micro-bubble streaming flows represent a unique type of actuating mechanism for microfluidics and have demonstrated great potential in the applications of micro-particle manipulation (e.g. size dependent trapping, sorting, and focusing etc.). The main object of this thesis work is to extend towards much smaller sized particle sorting down to 1μm and sub-micron. On top of that, we want to further explore the potential of applying bubble streaming sorting to biological objects which in the similar size scales. Systematical experiments have been conducted on polystyrene latex particles as well as several types biological cells and its infectious viruses, specifically Sulfolobus islandicus with Sulfolobus spindle-shaped viruses, and also Escherichia coli with Lambda phage. Size sensitive sorting has been revealed by means of larger sized objects will be majorly deflected away from their original streamline while smaller sized objects will follow, which leads to size-sensitive sorting. It has been proved that relative concentration ratio of two sized particle mixing solution can be enhanced with almost a factor of 6, and can be further improved by sequential bubble sorting. However, sorting application with smaller particles with diameter as 1μm and 100nm doesn’t perform as well as larger particles sorting reported by Wang et al. (Appl. Phys. Lett. 99, 034101, 2011) when they used a mixture of 10μm and 5μm particles in diameter. Fundamental reason of this can be explained by a combination of advection and diffusion effect. Beyond that, the principle of micro-bubble streaming sorting also has shown applicable to biological objects sorting purpose, but could be affected more by (i) wider size distribution, (ii) naturally morphologically diverse, (iii) active motion of live cells and (iv) more deformable compared to artificial polystyrene particles.","abstract_has_math":false,"creators":["Yang, Rui"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Mechanical Engineering","degree_department":null,"school":null,"contributors":["Hilgenfeldt, Sascha"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-03-02T19:45:14Z","date_published":"2016-03-02T19:45:14Z","updated_at":"2026-07-22T22:26:32Z","subjects":["Microfluidics","sorting","micron-bubble"],"languages":["en"],"rights":["Copyright 2015 Rui Yang"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/89091","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Hilgenfeldt, Sascha"]},{"key":"dc:creator","label":"Author","values":["Yang, Rui"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2016-03-02T19:45:14Z","2015-12-10","2015-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Mechanical Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Microfluidics","sorting","micron-bubble"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2015 Rui Yang"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/89091"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Micro-bubble streaming flows represent a unique type of actuating mechanism for microfluidics and have demonstrated great potential in the applications of micro-particle manipulation (e.g. size dependent trapping, sorting, and focusing etc.). The main object of this thesis work is to extend towards much smaller sized particle sorting down to 1μm and sub-micron. On top of that, we want to further explore the potential of applying bubble streaming sorting to biological objects which in the similar size scales. Systematical experiments have been conducted on polystyrene latex particles as well as several types biological cells and its infectious viruses, specifically Sulfolobus islandicus with Sulfolobus spindle-shaped viruses, and also Escherichia coli with Lambda phage. Size sensitive sorting has been revealed by means of larger sized objects will be majorly deflected away from their original streamline while smaller sized objects will follow, which leads to size-sensitive sorting. It has been proved that relative concentration ratio of two sized particle mixing solution can be enhanced with almost a factor of 6, and can be further improved by sequential bubble sorting. However, sorting application with smaller particles with diameter as 1μm and 100nm doesn’t perform as well as larger particles sorting reported by Wang et al. (Appl. Phys. Lett. 99, 034101, 2011) when they used a mixture of 10μm and 5μm particles in diameter. Fundamental reason of this can be explained by a combination of advection and diffusion effect. Beyond that, the principle of micro-bubble streaming sorting also has shown applicable to biological objects sorting purpose, but could be affected more by (i) wider size distribution, (ii) naturally morphologically diverse, (iii) active motion of live cells and (iv) more deformable compared to artificial polystyrene particles.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2016-03-02 without embargo terms","The student, Rui Yang, accepted the attached license on 2015-12-09 at 23:30.","The student, Rui Yang, submitted this Thesis for approval on 2015-12-10 at 00:01.","This Thesis was approved for publication on 2015-12-10 at 16:00.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8997 on 2016-03-02 at 12:53:08","Made available in DSpace on 2016-03-02T19:45:14Z (GMT). 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On top of that, we want to further explore the potential of applying bubble streaming sorting to biological objects which in the similar size scales. Systematical experiments have been conducted on polystyrene latex particles as well as several types biological cells and its infectious viruses, specifically Sulfolobus islandicus with Sulfolobus spindle-shaped viruses, and also Escherichia coli with Lambda phage. Size sensitive sorting has been revealed by means of larger sized objects will be majorly deflected away from their original streamline while smaller sized objects will follow, which leads to size-sensitive sorting. It has been proved that relative concentration ratio of two sized particle mixing solution can be enhanced with almost a factor of 6, and can be further improved by sequential bubble sorting. However, sorting application with smaller particles with diameter as 1μm and 100nm doesn’t perform as well as larger particles sorting reported by Wang et al. (Appl. Phys. Lett. 99, 034101, 2011) when they used a mixture of 10μm and 5μm particles in diameter. Fundamental reason of this can be explained by a combination of advection and diffusion effect. Beyond that, the principle of micro-bubble streaming sorting also has shown applicable to biological objects sorting purpose, but could be affected more by (i) wider size distribution, (ii) naturally morphologically diverse, (iii) active motion of live cells and (iv) more deformable compared to artificial polystyrene particles.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2016-03-02 without embargo terms","The student, Rui Yang, accepted the attached license on 2015-12-09 at 23:30.","The student, Rui Yang, submitted this Thesis for approval on 2015-12-10 at 00:01.","This Thesis was approved for publication on 2015-12-10 at 16:00.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8997 on 2016-03-02 at 12:53:08","Made available in DSpace on 2016-03-02T19:45:14Z (GMT). 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