{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/89059"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/89059","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Phosphorus bioavailability and digestibility in canola meals determined by different methods","abstract":"Six experiments were conducted to determine P bioavailability for a new test high-protein canola meal (TCM), a conventional canola meal (CCM) and dehulled soybean meal (SBM) using different types of animal assays. Experiment 1 was a chick-growth bioassay conducted to determine relative P bioavailability in the TCM, CCM, and SBM relative to KH2PO4. A phosphorus-deficient cornstarch-dextrose-SBM basal diet was fed as Diet 1. Diets 2 and 3 had 0.05% or 0.10% P added from KH2PO4, respectively. The remaining diets had 12.5% or 25% TCM, CCM, or SBM added in place of cornstarch and dextrose. Bioavailability of P was estimated using the multiple-regression slope-ratio method where tibia ash was regressed on supplemental P intake. A linear increase in tibia ash was observed as the P level was increased by addition of KH2PO4, CCM, TCM, or SBM. Mean bioavailability values of P in the TCM, CCM, and SBM relative to KH2PO4 were 18, 15, and 34%, respectively. It was concluded that the bioavailable P content of the new TCM was statistically equal to the CCM. Experiment 2, an additional chick bioassay, was conducted to determine the effect of phytase enzyme (Optiphos, Huvepharma, Sofia, Bulgaria) on bioavailability of the P in the CMs. Diet 1 was a phosphorus-deficient TCM-cornstarch-dextrose diet, with the TCM as the only source of dietary P. Diets 2-4 had 0.05%, 0.10%, or 0.15% P added from KH2PO4, respectively. Diets 5 and 6 were the same as Diet 1 with 125 or 250 units phytase added per kg of diets, respectively. Diets 7-14 were the same as Diets 1-7 except that CCM was the CM source. Multiple-regression was used to regress bone ash on supplemental P intake and the slope-ratio method was used to determine P release by phytase. It was estimated that the addition of 125 or 250 units/kg of phytase greatly increased the bioavailable P content of the TCM by 0.05 and 0.10%, respectively, and the response to phytase was slightly lower for the CCM than TCM. The results indicated that phytase greatly and similarly increased the bioavailability of P in the TCM and CCM. Experiment 3 was an ad-libitum-fed chick experiment which evaluated the effect of phytase on P digestibility and (ileal) retention (excreta) values for CCM based on ileal and excreta contents, respectively. The chicks were fed a P - deficient cornstarch - dextrose CM basal diet (.13% available P) as Diet 1. Diets 2 and 3 were the basal diet plus 125 or 250 FTU/kg of phytase, respectively. On Day 22, the ileal digesta and excreta were collected and analyzed for P. Ileal P digestibility was 38.0%, 44.8%, and 46.6% for birds fed Diets 1-3, respectively. The P retention values were determined to be 38.7%, 47.3%, and 51.0% for Diets 1-3, respectively. Experiment 4 was a precision - fed chick assay conducted to determine the ileal P digestibility of CCM. The chicks were fed a nutritionally complete corn - SBM starter diet from Days 1 - 20. On Day 21, after fasting for 9 hours overnight, the chicks were tube fed 3, 6, or 9 g for CCM. Ileal digesta were collected four hours after feeding. Mean ileal P digestibility was determined to be 47.5% in chicks fed 6 g and 40% in chicks fed 9 g of CCM. Experiment 5 was conducted using a precision-fed rooster assay to determine P retention values for CCM. After withdrawal of feed for 24 hours, the roosters were tube-fed 8, 16, or 24 g of CCM. All excreta (feces + urine) were collected 48 hours after feeding. Phosphorus standardized retention values were 34.6%, 28.5%, and 23.1% for birds tube-fed 8, 16, and 24 g of CCM, respectively. Experiment 6 was an ad libitum-fed chick assay to determine the ileal P digestibility and retention of CCM with and without increasing levels of dietary supplemental Ca and Ca: P ratio. The chicks were fed a P deficient - dextrose - CCM basal diet (0.039% available P, 13.50% CCM) as Diet 1. Diets 2-4 contained increasing levels of 27%, 40.50%, or 54% added CCM, respectively. The Ca: available P ratio was maintained at a 2:1 ratio in Diets 1-4. Diets 5-8 were the same as Diets 1-4 but supplemental calcium was added so that the Ca: available P ratio was maintained at a 6:1 ratio. Diets were fed from Days 15-21 posthatch and excreta were collected on Days 21 and 22 and ileal digesta on Day 22. Phosphorus digestibility decreased with each increase in dietary CCM and also with increased Ca: P ratio. Phosphorus retention values also generally decreased with increased Ca: P ratio. The results of Experiments 3-6 indicated the P digestibility and retention values for CCM varied among balance methods and sometimes among levels of CCM fed.","abstract_html":"Six experiments were conducted to determine P bioavailability for a new test high-protein canola meal (TCM), a conventional canola meal (CCM) and dehulled soybean meal (SBM) using different types of animal assays. Experiment 1 was a chick-growth bioassay conducted to determine relative P bioavailability in the TCM, CCM, and SBM relative to KH2PO4. A phosphorus-deficient cornstarch-dextrose-SBM basal diet was fed as Diet 1. Diets 2 and 3 had 0.05% or 0.10% P added from KH2PO4, respectively. The remaining diets had 12.5% or 25% TCM, CCM, or SBM added in place of cornstarch and dextrose. Bioavailability of P was estimated using the multiple-regression slope-ratio method where tibia ash was regressed on supplemental P intake. A linear increase in tibia ash was observed as the P level was increased by addition of KH2PO4, CCM, TCM, or SBM. Mean bioavailability values of P in the TCM, CCM, and SBM relative to KH2PO4 were 18, 15, and 34%, respectively. It was concluded that the bioavailable P content of the new TCM was statistically equal to the CCM. Experiment 2, an additional chick bioassay, was conducted to determine the effect of phytase enzyme (Optiphos, Huvepharma, Sofia, Bulgaria) on bioavailability of the P in the CMs. Diet 1 was a phosphorus-deficient TCM-cornstarch-dextrose diet, with the TCM as the only source of dietary P. Diets 2-4 had 0.05%, 0.10%, or 0.15% P added from KH2PO4, respectively. Diets 5 and 6 were the same as Diet 1 with 125 or 250 units phytase added per kg of diets, respectively. Diets 7-14 were the same as Diets 1-7 except that CCM was the CM source. Multiple-regression was used to regress bone ash on supplemental P intake and the slope-ratio method was used to determine P release by phytase. It was estimated that the addition of 125 or 250 units/kg of phytase greatly increased the bioavailable P content of the TCM by 0.05 and 0.10%, respectively, and the response to phytase was slightly lower for the CCM than TCM. The results indicated that phytase greatly and similarly increased the bioavailability of P in the TCM and CCM. Experiment 3 was an ad-libitum-fed chick experiment which evaluated the effect of phytase on P digestibility and (ileal) retention (excreta) values for CCM based on ileal and excreta contents, respectively. The chicks were fed a P - deficient cornstarch - dextrose CM basal diet (.13% available P) as Diet 1. Diets 2 and 3 were the basal diet plus 125 or 250 FTU/kg of phytase, respectively. On Day 22, the ileal digesta and excreta were collected and analyzed for P. Ileal P digestibility was 38.0%, 44.8%, and 46.6% for birds fed Diets 1-3, respectively. The P retention values were determined to be 38.7%, 47.3%, and 51.0% for Diets 1-3, respectively. Experiment 4 was a precision - fed chick assay conducted to determine the ileal P digestibility of CCM. The chicks were fed a nutritionally complete corn - SBM starter diet from Days 1 - 20. On Day 21, after fasting for 9 hours overnight, the chicks were tube fed 3, 6, or 9 g for CCM. Ileal digesta were collected four hours after feeding. Mean ileal P digestibility was determined to be 47.5% in chicks fed 6 g and 40% in chicks fed 9 g of CCM. Experiment 5 was conducted using a precision-fed rooster assay to determine P retention values for CCM. After withdrawal of feed for 24 hours, the roosters were tube-fed 8, 16, or 24 g of CCM. All excreta (feces + urine) were collected 48 hours after feeding. Phosphorus standardized retention values were 34.6%, 28.5%, and 23.1% for birds tube-fed 8, 16, and 24 g of CCM, respectively. Experiment 6 was an ad libitum-fed chick assay to determine the ileal P digestibility and retention of CCM with and without increasing levels of dietary supplemental Ca and Ca: P ratio. The chicks were fed a P deficient - dextrose - CCM basal diet (0.039% available P, 13.50% CCM) as Diet 1. Diets 2-4 contained increasing levels of 27%, 40.50%, or 54% added CCM, respectively. The Ca: available P ratio was maintained at a 2:1 ratio in Diets 1-4. Diets 5-8 were the same as Diets 1-4 but supplemental calcium was added so that the Ca: available P ratio was maintained at a 6:1 ratio. Diets were fed from Days 15-21 posthatch and excreta were collected on Days 21 and 22 and ileal digesta on Day 22. Phosphorus digestibility decreased with each increase in dietary CCM and also with increased Ca: P ratio. Phosphorus retention values also generally decreased with increased Ca: P ratio. The results of Experiments 3-6 indicated the P digestibility and retention values for CCM varied among balance methods and sometimes among levels of CCM fed.","abstract_has_math":false,"creators":["Hanna, Christina Denise"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Animal Sciences","degree_department":null,"school":null,"contributors":["Parsons, Carl M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-03-02T19:34:28Z","date_published":"2016-03-02T19:34:28Z","updated_at":"2026-07-22T22:26:32Z","subjects":["phosphorus","canola meal"],"languages":["en"],"rights":["Copyright 2015 Christina Hanna"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/89059","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Parsons, Carl M."]},{"key":"dc:creator","label":"Author","values":["Hanna, Christina Denise"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2016-03-02T19:34:28Z","2015-12-08","2015-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["phosphorus","canola meal"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2015 Christina Hanna"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/89059"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Six experiments were conducted to determine P bioavailability for a new test high-protein canola meal (TCM), a conventional canola meal (CCM) and dehulled soybean meal (SBM) using different types of animal assays. Experiment 1 was a chick-growth bioassay conducted to determine relative P bioavailability in the TCM, CCM, and SBM relative to KH2PO4. A phosphorus-deficient cornstarch-dextrose-SBM basal diet was fed as Diet 1. Diets 2 and 3 had 0.05% or 0.10% P added from KH2PO4, respectively. The remaining diets had 12.5% or 25% TCM, CCM, or SBM added in place of cornstarch and dextrose. Bioavailability of P was estimated using the multiple-regression slope-ratio method where tibia ash was regressed on supplemental P intake. A linear increase in tibia ash was observed as the P level was increased by addition of KH2PO4, CCM, TCM, or SBM. Mean bioavailability values of P in the TCM, CCM, and SBM relative to KH2PO4 were 18, 15, and 34%, respectively. It was concluded that the bioavailable P content of the new TCM was statistically equal to the CCM. Experiment 2, an additional chick bioassay, was conducted to determine the effect of phytase enzyme (Optiphos, Huvepharma, Sofia, Bulgaria) on bioavailability of the P in the CMs. Diet 1 was a phosphorus-deficient TCM-cornstarch-dextrose diet, with the TCM as the only source of dietary P. Diets 2-4 had 0.05%, 0.10%, or 0.15% P added from KH2PO4, respectively. Diets 5 and 6 were the same as Diet 1 with 125 or 250 units phytase added per kg of diets, respectively. Diets 7-14 were the same as Diets 1-7 except that CCM was the CM source. Multiple-regression was used to regress bone ash on supplemental P intake and the slope-ratio method was used to determine P release by phytase. It was estimated that the addition of 125 or 250 units/kg of phytase greatly increased the bioavailable P content of the TCM by 0.05 and 0.10%, respectively, and the response to phytase was slightly lower for the CCM than TCM. The results indicated that phytase greatly and similarly increased the bioavailability of P in the TCM and CCM. Experiment 3 was an ad-libitum-fed chick experiment which evaluated the effect of phytase on P digestibility and (ileal) retention (excreta) values for CCM based on ileal and excreta contents, respectively. The chicks were fed a P - deficient cornstarch - dextrose CM basal diet (.13% available P) as Diet 1. Diets 2 and 3 were the basal diet plus 125 or 250 FTU/kg of phytase, respectively. On Day 22, the ileal digesta and excreta were collected and analyzed for P. Ileal P digestibility was 38.0%, 44.8%, and 46.6% for birds fed Diets 1-3, respectively. The P retention values were determined to be 38.7%, 47.3%, and 51.0% for Diets 1-3, respectively. Experiment 4 was a precision - fed chick assay conducted to determine the ileal P digestibility of CCM. The chicks were fed a nutritionally complete corn - SBM starter diet from Days 1 - 20. On Day 21, after fasting for 9 hours overnight, the chicks were tube fed 3, 6, or 9 g for CCM. Ileal digesta were collected four hours after feeding. Mean ileal P digestibility was determined to be 47.5% in chicks fed 6 g and 40% in chicks fed 9 g of CCM. Experiment 5 was conducted using a precision-fed rooster assay to determine P retention values for CCM. After withdrawal of feed for 24 hours, the roosters were tube-fed 8, 16, or 24 g of CCM. All excreta (feces + urine) were collected 48 hours after feeding. Phosphorus standardized retention values were 34.6%, 28.5%, and 23.1% for birds tube-fed 8, 16, and 24 g of CCM, respectively. Experiment 6 was an ad libitum-fed chick assay to determine the ileal P digestibility and retention of CCM with and without increasing levels of dietary supplemental Ca and Ca: P ratio. The chicks were fed a P deficient - dextrose - CCM basal diet (0.039% available P, 13.50% CCM) as Diet 1. Diets 2-4 contained increasing levels of 27%, 40.50%, or 54% added CCM, respectively. The Ca: available P ratio was maintained at a 2:1 ratio in Diets 1-4. Diets 5-8 were the same as Diets 1-4 but supplemental calcium was added so that the Ca: available P ratio was maintained at a 6:1 ratio. Diets were fed from Days 15-21 posthatch and excreta were collected on Days 21 and 22 and ileal digesta on Day 22. Phosphorus digestibility decreased with each increase in dietary CCM and also with increased Ca: P ratio. Phosphorus retention values also generally decreased with increased Ca: P ratio. The results of Experiments 3-6 indicated the P digestibility and retention values for CCM varied among balance methods and sometimes among levels of CCM fed.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2016-03-02 without embargo terms","The student, Christina Hanna, accepted the attached license on 2015-12-07 at 11:51.","The student, Christina Hanna, submitted this Thesis for approval on 2015-12-07 at 11:51.","This Thesis was approved for publication on 2015-12-08 at 14:50.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8948 on 2016-03-02 at 12:51:43","Made available in DSpace on 2016-03-02T19:34:28Z (GMT). No. of bitstreams: 2 HANNA-THESIS-2015.pdf: 570446 bytes, checksum: 9a08b212ac9c179fc0ded1f66144e8e3 (MD5) LICENSE.txt: 4212 bytes, checksum: 66454b206b26dc688088a63df069d26a (MD5) Previous issue date: 2015-12-08"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Phosphorus bioavailability and digestibility in canola meals determined by different methods"]}]}],"canonical_facts":{"dc:contributor":["Parsons, Carl M."],"dc:creator":["Hanna, Christina Denise"],"dc:date":["2016-03-02T19:34:28Z","2015-12-08","2015-12"],"dc:description":["Six experiments were conducted to determine P bioavailability for a new test high-protein canola meal (TCM), a conventional canola meal (CCM) and dehulled soybean meal (SBM) using different types of animal assays. Experiment 1 was a chick-growth bioassay conducted to determine relative P bioavailability in the TCM, CCM, and SBM relative to KH2PO4. A phosphorus-deficient cornstarch-dextrose-SBM basal diet was fed as Diet 1. Diets 2 and 3 had 0.05% or 0.10% P added from KH2PO4, respectively. The remaining diets had 12.5% or 25% TCM, CCM, or SBM added in place of cornstarch and dextrose. Bioavailability of P was estimated using the multiple-regression slope-ratio method where tibia ash was regressed on supplemental P intake. A linear increase in tibia ash was observed as the P level was increased by addition of KH2PO4, CCM, TCM, or SBM. Mean bioavailability values of P in the TCM, CCM, and SBM relative to KH2PO4 were 18, 15, and 34%, respectively. It was concluded that the bioavailable P content of the new TCM was statistically equal to the CCM. Experiment 2, an additional chick bioassay, was conducted to determine the effect of phytase enzyme (Optiphos, Huvepharma, Sofia, Bulgaria) on bioavailability of the P in the CMs. Diet 1 was a phosphorus-deficient TCM-cornstarch-dextrose diet, with the TCM as the only source of dietary P. Diets 2-4 had 0.05%, 0.10%, or 0.15% P added from KH2PO4, respectively. Diets 5 and 6 were the same as Diet 1 with 125 or 250 units phytase added per kg of diets, respectively. Diets 7-14 were the same as Diets 1-7 except that CCM was the CM source. Multiple-regression was used to regress bone ash on supplemental P intake and the slope-ratio method was used to determine P release by phytase. It was estimated that the addition of 125 or 250 units/kg of phytase greatly increased the bioavailable P content of the TCM by 0.05 and 0.10%, respectively, and the response to phytase was slightly lower for the CCM than TCM. The results indicated that phytase greatly and similarly increased the bioavailability of P in the TCM and CCM. Experiment 3 was an ad-libitum-fed chick experiment which evaluated the effect of phytase on P digestibility and (ileal) retention (excreta) values for CCM based on ileal and excreta contents, respectively. The chicks were fed a P - deficient cornstarch - dextrose CM basal diet (.13% available P) as Diet 1. Diets 2 and 3 were the basal diet plus 125 or 250 FTU/kg of phytase, respectively. On Day 22, the ileal digesta and excreta were collected and analyzed for P. Ileal P digestibility was 38.0%, 44.8%, and 46.6% for birds fed Diets 1-3, respectively. The P retention values were determined to be 38.7%, 47.3%, and 51.0% for Diets 1-3, respectively. Experiment 4 was a precision - fed chick assay conducted to determine the ileal P digestibility of CCM. The chicks were fed a nutritionally complete corn - SBM starter diet from Days 1 - 20. On Day 21, after fasting for 9 hours overnight, the chicks were tube fed 3, 6, or 9 g for CCM. Ileal digesta were collected four hours after feeding. Mean ileal P digestibility was determined to be 47.5% in chicks fed 6 g and 40% in chicks fed 9 g of CCM. Experiment 5 was conducted using a precision-fed rooster assay to determine P retention values for CCM. After withdrawal of feed for 24 hours, the roosters were tube-fed 8, 16, or 24 g of CCM. All excreta (feces + urine) were collected 48 hours after feeding. Phosphorus standardized retention values were 34.6%, 28.5%, and 23.1% for birds tube-fed 8, 16, and 24 g of CCM, respectively. Experiment 6 was an ad libitum-fed chick assay to determine the ileal P digestibility and retention of CCM with and without increasing levels of dietary supplemental Ca and Ca: P ratio. The chicks were fed a P deficient - dextrose - CCM basal diet (0.039% available P, 13.50% CCM) as Diet 1. Diets 2-4 contained increasing levels of 27%, 40.50%, or 54% added CCM, respectively. The Ca: available P ratio was maintained at a 2:1 ratio in Diets 1-4. Diets 5-8 were the same as Diets 1-4 but supplemental calcium was added so that the Ca: available P ratio was maintained at a 6:1 ratio. Diets were fed from Days 15-21 posthatch and excreta were collected on Days 21 and 22 and ileal digesta on Day 22. Phosphorus digestibility decreased with each increase in dietary CCM and also with increased Ca: P ratio. Phosphorus retention values also generally decreased with increased Ca: P ratio. The results of Experiments 3-6 indicated the P digestibility and retention values for CCM varied among balance methods and sometimes among levels of CCM fed.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2016-03-02 without embargo terms","The student, Christina Hanna, accepted the attached license on 2015-12-07 at 11:51.","The student, Christina Hanna, submitted this Thesis for approval on 2015-12-07 at 11:51.","This Thesis was approved for publication on 2015-12-08 at 14:50.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8948 on 2016-03-02 at 12:51:43","Made available in DSpace on 2016-03-02T19:34:28Z (GMT). No. of bitstreams: 2 HANNA-THESIS-2015.pdf: 570446 bytes, checksum: 9a08b212ac9c179fc0ded1f66144e8e3 (MD5) LICENSE.txt: 4212 bytes, checksum: 66454b206b26dc688088a63df069d26a (MD5) Previous issue date: 2015-12-08"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/89059"],"dc:language":["en"],"dc:rights":["Copyright 2015 Christina Hanna"],"dc:subject":["phosphorus","canola meal"],"dc:title":["Phosphorus bioavailability and digestibility in canola meals determined by different methods"],"dc:type":["text"],"thesis:degree_discipline":["Animal Sciences"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:32Z"}