{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/88311"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/88311","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"A bioengineered approach for sustainable crude oil pollution treatment via entrapment, dispersal and removal using Nano-CarboScavenger","abstract":"Aqueous petroleum contamination continues to threaten life-dependent bodies of water and surrounding ecosystems including human habitation. It is now well established that most methods of petroleum remediation are either inefficient or involve highly toxic chemical dispersants. In this work we adopt a commercially-amenable nanotechnology based approach to design tunable, biodegradable particles for efficient and safe petroleum remediation. Exhaustively characterized 100 nm sized hybrid dual-shelled polymeric (PS275-b-PAA50) and carbon-cored nanoarchitectures (Nano-CarboScavengers or NCS) were derived predominantly from natural carbohydrate sources and designed for a multipurpose oil sequestration and dispersion agent that is environmentally undisruptive. NCS distribution on crude oil contaminated water was found to combine both absorption and dispersion mechanisms to treat a maximum of 80% of the pollution, while NCS distribution on a petroleum distillate mixture efficiently treated 91% of the pollution by absorption alone. NCS exposure to Zebrafish, Vibrio fischeri, and MCF-7 were performed in vitro and were found to be remarkably un‐inhibitive with LD50: 8 g/L, EC50: 1.28 g/L and IC50: 4 g/L respectively. This far exceeds expected NCS exposure (ENE) of 1x10-5 g/L in bodies of water. Finally, NCS degraded in the presence of human myeloperoxidase (HMPO) and horseradish peroxidase (HRP), suggesting any incidental biological uptake can be enzymatically digested in living systems.","abstract_html":"Aqueous petroleum contamination continues to threaten life-dependent bodies of water and surrounding ecosystems including human habitation. It is now well established that most methods of petroleum remediation are either inefficient or involve highly toxic chemical dispersants. In this work we adopt a commercially-amenable nanotechnology based approach to design tunable, biodegradable particles for efficient and safe petroleum remediation. Exhaustively characterized 100 nm sized hybrid dual-shelled polymeric (PS275-b-PAA50) and carbon-cored nanoarchitectures (Nano-CarboScavengers or NCS) were derived predominantly from natural carbohydrate sources and designed for a multipurpose oil sequestration and dispersion agent that is environmentally undisruptive. NCS distribution on crude oil contaminated water was found to combine both absorption and dispersion mechanisms to treat a maximum of 80% of the pollution, while NCS distribution on a petroleum distillate mixture efficiently treated 91% of the pollution by absorption alone. NCS exposure to Zebrafish, Vibrio fischeri, and MCF-7 were performed in vitro and were found to be remarkably un‐inhibitive with LD50: 8 g/L, EC50: 1.28 g/L and IC50: 4 g/L respectively. This far exceeds expected NCS exposure (ENE) of 1x10-5 g/L in bodies of water. Finally, NCS degraded in the presence of human myeloperoxidase (HMPO) and horseradish peroxidase (HRP), suggesting any incidental biological uptake can be enzymatically digested in living systems.","abstract_has_math":false,"creators":["Daza, Enrique A."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Pan, Dipanjan"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-29T21:08:23Z","date_published":"2015-09-29T21:08:23Z","updated_at":"2026-07-22T22:26:32Z","subjects":["crude oil","petroleum","spill","oil","nanotechnology","sustainability","Nano-CarboScavenger","dispersion","dispersant","absorption","nanoparticle","bioengineering"],"languages":["en"],"rights":["Copyright 2015 Enrique Daza"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/88311","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Pan, Dipanjan"]},{"key":"dc:creator","label":"Author","values":["Daza, Enrique A."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-29T21:08:23Z","2017-09-30T09:15:18Z","2015-08","2015-07-22","2015-8"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["crude oil","petroleum","spill","oil","nanotechnology","sustainability","Nano-CarboScavenger","dispersion","dispersant","absorption","nanoparticle","bioengineering"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2015 Enrique Daza"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/88311"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Aqueous petroleum contamination continues to threaten life-dependent bodies of water and surrounding ecosystems including human habitation. It is now well established that most methods of petroleum remediation are either inefficient or involve highly toxic chemical dispersants. In this work we adopt a commercially-amenable nanotechnology based approach to design tunable, biodegradable particles for efficient and safe petroleum remediation. Exhaustively characterized 100 nm sized hybrid dual-shelled polymeric (PS275-b-PAA50) and carbon-cored nanoarchitectures (Nano-CarboScavengers or NCS) were derived predominantly from natural carbohydrate sources and designed for a multipurpose oil sequestration and dispersion agent that is environmentally undisruptive. NCS distribution on crude oil contaminated water was found to combine both absorption and dispersion mechanisms to treat a maximum of 80% of the pollution, while NCS distribution on a petroleum distillate mixture efficiently treated 91% of the pollution by absorption alone. NCS exposure to Zebrafish, Vibrio fischeri, and MCF-7 were performed in vitro and were found to be remarkably un‐inhibitive with LD50: 8 g/L, EC50: 1.28 g/L and IC50: 4 g/L respectively. This far exceeds expected NCS exposure (ENE) of 1x10-5 g/L in bodies of water. Finally, NCS degraded in the presence of human myeloperoxidase (HMPO) and horseradish peroxidase (HRP), suggesting any incidental biological uptake can be enzymatically digested in living systems.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2017-08-01","The student, Enrique Daza, accepted the attached license on 2015-07-21 at 22:47.","The student, Enrique Daza, submitted this Thesis for approval on 2015-07-21 at 23:02.","This Thesis was approved for publication on 2015-07-22 at 13:33.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8600 on 2015-09-29 at 15:06:49","Made available in DSpace on 2015-09-29T21:08:23Z (GMT). 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It is now well established that most methods of petroleum remediation are either inefficient or involve highly toxic chemical dispersants. In this work we adopt a commercially-amenable nanotechnology based approach to design tunable, biodegradable particles for efficient and safe petroleum remediation. Exhaustively characterized 100 nm sized hybrid dual-shelled polymeric (PS275-b-PAA50) and carbon-cored nanoarchitectures (Nano-CarboScavengers or NCS) were derived predominantly from natural carbohydrate sources and designed for a multipurpose oil sequestration and dispersion agent that is environmentally undisruptive. NCS distribution on crude oil contaminated water was found to combine both absorption and dispersion mechanisms to treat a maximum of 80% of the pollution, while NCS distribution on a petroleum distillate mixture efficiently treated 91% of the pollution by absorption alone. NCS exposure to Zebrafish, Vibrio fischeri, and MCF-7 were performed in vitro and were found to be remarkably un‐inhibitive with LD50: 8 g/L, EC50: 1.28 g/L and IC50: 4 g/L respectively. This far exceeds expected NCS exposure (ENE) of 1x10-5 g/L in bodies of water. Finally, NCS degraded in the presence of human myeloperoxidase (HMPO) and horseradish peroxidase (HRP), suggesting any incidental biological uptake can be enzymatically digested in living systems.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2017-08-01","The student, Enrique Daza, accepted the attached license on 2015-07-21 at 22:47.","The student, Enrique Daza, submitted this Thesis for approval on 2015-07-21 at 23:02.","This Thesis was approved for publication on 2015-07-22 at 13:33.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8600 on 2015-09-29 at 15:06:49","Made available in DSpace on 2015-09-29T21:08:23Z (GMT). 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