{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/88272"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/88272","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Transport of soft materials in crowded environment","abstract":"Fluorescent microscopy was used to study the transport of soft material objects in crowded environment. Delaunay triangulation and wavelet transform were adapted to extract more information from images of macromolecules with irregular shapes and heterogeneous transportation dynamics. Systems studied are actively transported endosomes in living cells, and diffusing semi-flexible polymer chains in rigid networks. By going beyond traditional particle tracking and trajectory analysis, it was discovered that for protein transportation rather than directing the protein-containing endosomes steadily towards intended destination with regulatory mechanisms as commonly believed, efficient random search is an alternative mechanism that can offer both high energy efficiency and delivery accuracy. The imaging study of double-stranded DNA molecules in actin and agarose gel showed that the popular mental image of a snake sliding back and forth may not be how polymers actually reptate. The application of advanced analytical tools to high resolution microscopy images of dynamic systems is expected to lead to the discoveries of many more new mechanisms and concepts.","abstract_html":"Fluorescent microscopy was used to study the transport of soft material objects in crowded environment. Delaunay triangulation and wavelet transform were adapted to extract more information from images of macromolecules with irregular shapes and heterogeneous transportation dynamics. Systems studied are actively transported endosomes in living cells, and diffusing semi-flexible polymer chains in rigid networks. By going beyond traditional particle tracking and trajectory analysis, it was discovered that for protein transportation rather than directing the protein-containing endosomes steadily towards intended destination with regulatory mechanisms as commonly believed, efficient random search is an alternative mechanism that can offer both high energy efficiency and delivery accuracy. The imaging study of double-stranded DNA molecules in actin and agarose gel showed that the popular mental image of a snake sliding back and forth may not be how polymers actually reptate. The application of advanced analytical tools to high resolution microscopy images of dynamic systems is expected to lead to the discoveries of many more new mechanisms and concepts.","abstract_has_math":false,"creators":["Chen, Kejia"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemical Engineering","degree_department":null,"school":null,"contributors":["Granick, Steve","Schroeder, Charles M.","Freund, L B","Kraft, Mary","Ferguson, Andrew"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-29T21:03:09Z","date_published":"2015-09-29T21:03:09Z","updated_at":"2026-07-22T22:26:31Z","subjects":["wavelet transform","delaunay triangulation","particle tracking","protein active transport"],"languages":["en"],"rights":["Copyright 2015 Kejia Chen"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/88272","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Granick, Steve","Schroeder, Charles M.","Freund, L B","Kraft, Mary","Ferguson, Andrew"]},{"key":"dc:creator","label":"Author","values":["Chen, Kejia"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-29T21:03:09Z","2017-09-30T09:15:32Z","2015-08","2015-07-10","2015-8"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemical Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["wavelet transform","delaunay triangulation","particle tracking","protein active transport"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2015 Kejia Chen"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/88272"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Fluorescent microscopy was used to study the transport of soft material objects in crowded environment. Delaunay triangulation and wavelet transform were adapted to extract more information from images of macromolecules with irregular shapes and heterogeneous transportation dynamics. Systems studied are actively transported endosomes in living cells, and diffusing semi-flexible polymer chains in rigid networks. By going beyond traditional particle tracking and trajectory analysis, it was discovered that for protein transportation rather than directing the protein-containing endosomes steadily towards intended destination with regulatory mechanisms as commonly believed, efficient random search is an alternative mechanism that can offer both high energy efficiency and delivery accuracy. The imaging study of double-stranded DNA molecules in actin and agarose gel showed that the popular mental image of a snake sliding back and forth may not be how polymers actually reptate. The application of advanced analytical tools to high resolution microscopy images of dynamic systems is expected to lead to the discoveries of many more new mechanisms and concepts.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2017-08-01","The student, Kejia Chen, accepted the attached license on 2015-07-09 at 14:43.","The student, Kejia Chen, submitted this Dissertation for approval on 2015-07-09 at 14:51.","This Dissertation was approved for publication on 2015-07-10 at 09:59.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8390 on 2015-09-29 at 15:05:53","Made available in DSpace on 2015-09-29T21:03:09Z (GMT). No. of bitstreams: 2 CHEN-DISSERTATION-2015.pdf: 3741549 bytes, checksum: ada7cf663bd8419c0ea87afa7218ee5b (MD5) LICENSE.txt: 4207 bytes, checksum: 4546923dd5ddb9071dd3a85ef2991d7e (MD5) Previous issue date: 2015-07-10","Embargo set by: Seth Robbins for item 89552 Lift date: 2017-09-29T21:03:28Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 89552 Lift date: 2017-09-29T21:08:35Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 89552 on 2017-09-30T09:15:32Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Transport of soft materials in crowded environment"]}]}],"canonical_facts":{"dc:contributor":["Granick, Steve","Schroeder, Charles M.","Freund, L B","Kraft, Mary","Ferguson, Andrew"],"dc:creator":["Chen, Kejia"],"dc:date":["2015-09-29T21:03:09Z","2017-09-30T09:15:32Z","2015-08","2015-07-10","2015-8"],"dc:description":["Fluorescent microscopy was used to study the transport of soft material objects in crowded environment. Delaunay triangulation and wavelet transform were adapted to extract more information from images of macromolecules with irregular shapes and heterogeneous transportation dynamics. Systems studied are actively transported endosomes in living cells, and diffusing semi-flexible polymer chains in rigid networks. By going beyond traditional particle tracking and trajectory analysis, it was discovered that for protein transportation rather than directing the protein-containing endosomes steadily towards intended destination with regulatory mechanisms as commonly believed, efficient random search is an alternative mechanism that can offer both high energy efficiency and delivery accuracy. The imaging study of double-stranded DNA molecules in actin and agarose gel showed that the popular mental image of a snake sliding back and forth may not be how polymers actually reptate. The application of advanced analytical tools to high resolution microscopy images of dynamic systems is expected to lead to the discoveries of many more new mechanisms and concepts.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2017-08-01","The student, Kejia Chen, accepted the attached license on 2015-07-09 at 14:43.","The student, Kejia Chen, submitted this Dissertation for approval on 2015-07-09 at 14:51.","This Dissertation was approved for publication on 2015-07-10 at 09:59.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8390 on 2015-09-29 at 15:05:53","Made available in DSpace on 2015-09-29T21:03:09Z (GMT). No. of bitstreams: 2 CHEN-DISSERTATION-2015.pdf: 3741549 bytes, checksum: ada7cf663bd8419c0ea87afa7218ee5b (MD5) LICENSE.txt: 4207 bytes, checksum: 4546923dd5ddb9071dd3a85ef2991d7e (MD5) Previous issue date: 2015-07-10","Embargo set by: Seth Robbins for item 89552 Lift date: 2017-09-29T21:03:28Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 89552 Lift date: 2017-09-29T21:08:35Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 89552 on 2017-09-30T09:15:32Z."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/88272"],"dc:language":["en"],"dc:rights":["Copyright 2015 Kejia Chen"],"dc:subject":["wavelet transform","delaunay triangulation","particle tracking","protein active transport"],"dc:title":["Transport of soft materials in crowded environment"],"dc:type":["text"],"thesis:degree_discipline":["Chemical Engineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:31Z"}