{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/88258"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/88258","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Matrix-mediated formation & innervation of skeletal myotubes","abstract":"The field of tissue engineering utilizes combinations of cells, biomaterials, and therapeutic molecules in order to generate therapies and better understand the emergent behaviors involved in tissue formation and diseases. One of the goals of tissue engineering is to be able to recapitulate the in vivo microenvironment of cells in vitro. As such, a lot of attention has been focused on the development of materials that are able to present various aspects of the cell microenvironment so that the specific roles of each property can be probed. The overall goal of this thesis is to develop advanced matrices for the formation and innervation of skeletal muscle. This thesis discusses the development of advanced bioactive matrices to be able to study the role of a variety of extracellular matrix properties on skeletal muscle formation and, ultimately, innervation. The role of polysaccharide mediated pores will be investigated in Chapter 2. Myogenic differentiation will be decoupled from matrix stiffness in an aligned microchanneled matrix in Chapter 3. In Chapter 4, the role of matrix stiffness on neural organoid development and, its ability to innervate and control the actuation of skeletal muscle will be investigated. The results of this thesis will be useful in developing a better understanding of how matrix properties mediate skeletal myotube formation and innervation. Additionally, the results will provide a method to be able to study the emergent behaviors involved in myotube formation and neuromuscular diseases.","abstract_html":"The field of tissue engineering utilizes combinations of cells, biomaterials, and therapeutic molecules in order to generate therapies and better understand the emergent behaviors involved in tissue formation and diseases. One of the goals of tissue engineering is to be able to recapitulate the in vivo microenvironment of cells in vitro. As such, a lot of attention has been focused on the development of materials that are able to present various aspects of the cell microenvironment so that the specific roles of each property can be probed. The overall goal of this thesis is to develop advanced matrices for the formation and innervation of skeletal muscle. This thesis discusses the development of advanced bioactive matrices to be able to study the role of a variety of extracellular matrix properties on skeletal muscle formation and, ultimately, innervation. The role of polysaccharide mediated pores will be investigated in Chapter 2. Myogenic differentiation will be decoupled from matrix stiffness in an aligned microchanneled matrix in Chapter 3. In Chapter 4, the role of matrix stiffness on neural organoid development and, its ability to innervate and control the actuation of skeletal muscle will be investigated. The results of this thesis will be useful in developing a better understanding of how matrix properties mediate skeletal myotube formation and innervation. Additionally, the results will provide a method to be able to study the emergent behaviors involved in myotube formation and neuromuscular diseases.","abstract_has_math":false,"creators":["Rich, Max Harrison"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemical Engineering","degree_department":null,"school":null,"contributors":["Kong, Hyun Joon","Gillette, Martha U.","Leckband, Deborah E.","Zhao, Huimin"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-29T21:02:59Z","date_published":"2015-09-29T21:02:59Z","updated_at":"2026-07-22T22:26:31Z","subjects":["neuromuscular junction","skeletal myotubes","functional neuromuscular junctions","biomaterials"],"languages":["en"],"rights":["Copyright 2015 Max Harrison Rich"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/88258","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kong, Hyun Joon","Gillette, Martha U.","Leckband, Deborah E.","Zhao, Huimin"]},{"key":"dc:creator","label":"Author","values":["Rich, Max Harrison"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-29T21:02:59Z","2017-09-30T09:15:18Z","2015-08","2015-07-06","2015-8"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemical Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["neuromuscular junction","skeletal myotubes","functional neuromuscular junctions","biomaterials"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2015 Max Harrison Rich"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/88258"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The field of tissue engineering utilizes combinations of cells, biomaterials, and therapeutic molecules in order to generate therapies and better understand the emergent behaviors involved in tissue formation and diseases. One of the goals of tissue engineering is to be able to recapitulate the in vivo microenvironment of cells in vitro. As such, a lot of attention has been focused on the development of materials that are able to present various aspects of the cell microenvironment so that the specific roles of each property can be probed. The overall goal of this thesis is to develop advanced matrices for the formation and innervation of skeletal muscle. This thesis discusses the development of advanced bioactive matrices to be able to study the role of a variety of extracellular matrix properties on skeletal muscle formation and, ultimately, innervation. The role of polysaccharide mediated pores will be investigated in Chapter 2. Myogenic differentiation will be decoupled from matrix stiffness in an aligned microchanneled matrix in Chapter 3. In Chapter 4, the role of matrix stiffness on neural organoid development and, its ability to innervate and control the actuation of skeletal muscle will be investigated. The results of this thesis will be useful in developing a better understanding of how matrix properties mediate skeletal myotube formation and innervation. Additionally, the results will provide a method to be able to study the emergent behaviors involved in myotube formation and neuromuscular diseases.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2017-08-01","The student, Max Rich, accepted the attached license on 2015-07-03 at 13:16.","The student, Max Rich, submitted this Dissertation for approval on 2015-07-03 at 13:32.","This Dissertation was approved for publication on 2015-07-06 at 15:21.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8341 on 2015-09-29 at 15:05:33","Made available in DSpace on 2015-09-29T21:02:59Z (GMT). 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One of the goals of tissue engineering is to be able to recapitulate the in vivo microenvironment of cells in vitro. As such, a lot of attention has been focused on the development of materials that are able to present various aspects of the cell microenvironment so that the specific roles of each property can be probed. The overall goal of this thesis is to develop advanced matrices for the formation and innervation of skeletal muscle. This thesis discusses the development of advanced bioactive matrices to be able to study the role of a variety of extracellular matrix properties on skeletal muscle formation and, ultimately, innervation. The role of polysaccharide mediated pores will be investigated in Chapter 2. Myogenic differentiation will be decoupled from matrix stiffness in an aligned microchanneled matrix in Chapter 3. In Chapter 4, the role of matrix stiffness on neural organoid development and, its ability to innervate and control the actuation of skeletal muscle will be investigated. The results of this thesis will be useful in developing a better understanding of how matrix properties mediate skeletal myotube formation and innervation. Additionally, the results will provide a method to be able to study the emergent behaviors involved in myotube formation and neuromuscular diseases.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2017-08-01","The student, Max Rich, accepted the attached license on 2015-07-03 at 13:16.","The student, Max Rich, submitted this Dissertation for approval on 2015-07-03 at 13:32.","This Dissertation was approved for publication on 2015-07-06 at 15:21.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8341 on 2015-09-29 at 15:05:33","Made available in DSpace on 2015-09-29T21:02:59Z (GMT). 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