{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/88116"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/88116","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Zein films as substrates for cell attachment","abstract":"Circulating tumor cells (CTCs) are cells that have shed into the vasculature from a primary tumor and circulate in the blood stream. Circulating tumor cells play an important role in metastasis. The long term goal of this project is to capture and remocve CTC’s from the blood stream. Numerous studies on glutamine metabolism in cancer indicate that many tumors are avid glutamine consumers in vivo and in vitro. Prolamins are a group of plant seed proteins having high glutamine content. A main example is corn zein. Zein has been reported to support cell attachment. The main hypothesis in this work was that zein is able to support attachment of cancer (HeLa) cells. In this work, HeLa cells were grown on glass slides coated with zein films. It was reported that the hydrophobicity and topography of substrates are important for cell attachment. Therefore, a secondary hypothesis was that the hydrophobicity and topography of zein substrates could be optimized for cancer cell attachment. The hydrophobicity of zein films had been observed to reflect the hydrophobicity of its underlying substrate. Thus, glass slides were treated with oxygen plasma to modulate their hydrophobicity. The surface hydrophobicity of substrates intended for cell attachment was measured with a CAM 200 Goniometer. Topographic features were examined by MFP-3D AFM. Results showed that HeLa cells were observed to adhere and grow on zein substrates cast on oxygen treated plasma glass slides. The glutamine effect of zein on HeLa cells was evidenced by the cells unfavorable response when zein was deamidated by surface treatment with hydrochloric acid. HeLa cells on zein films developed elongated cells at 24h and 48 h of incubation. Zein was believed to support HeLa cells attachment. Results of this work are expected to be useful in designing bio surfaces for isolation of cancer cells.","abstract_html":"Circulating tumor cells (CTCs) are cells that have shed into the vasculature from a primary tumor and circulate in the blood stream. Circulating tumor cells play an important role in metastasis. The long term goal of this project is to capture and remocve CTC’s from the blood stream. Numerous studies on glutamine metabolism in cancer indicate that many tumors are avid glutamine consumers in vivo and in vitro. Prolamins are a group of plant seed proteins having high glutamine content. A main example is corn zein. Zein has been reported to support cell attachment. The main hypothesis in this work was that zein is able to support attachment of cancer (HeLa) cells. In this work, HeLa cells were grown on glass slides coated with zein films. It was reported that the hydrophobicity and topography of substrates are important for cell attachment. Therefore, a secondary hypothesis was that the hydrophobicity and topography of zein substrates could be optimized for cancer cell attachment. The hydrophobicity of zein films had been observed to reflect the hydrophobicity of its underlying substrate. Thus, glass slides were treated with oxygen plasma to modulate their hydrophobicity. The surface hydrophobicity of substrates intended for cell attachment was measured with a CAM 200 Goniometer. Topographic features were examined by MFP-3D AFM. Results showed that HeLa cells were observed to adhere and grow on zein substrates cast on oxygen treated plasma glass slides. The glutamine effect of zein on HeLa cells was evidenced by the cells unfavorable response when zein was deamidated by surface treatment with hydrochloric acid. HeLa cells on zein films developed elongated cells at 24h and 48 h of incubation. Zein was believed to support HeLa cells attachment. Results of this work are expected to be useful in designing bio surfaces for isolation of cancer cells.","abstract_has_math":false,"creators":["Qin, Ying"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Food Science & Human Nutrition","degree_department":null,"school":null,"contributors":["Padua, Graciela W."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-29T20:38:53Z","date_published":"2015-09-29T20:38:53Z","updated_at":"2026-07-22T22:26:31Z","subjects":["zein films","HeLa cells"],"languages":["en"],"rights":["Copyright 2015 Ying Qin"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/88116","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Padua, Graciela W."]},{"key":"dc:creator","label":"Author","values":["Qin, Ying"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-29T20:38:53Z","2015-08","2015-07-24","2015-8"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science & Human Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["zein films","HeLa cells"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2015 Ying Qin"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/88116"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Circulating tumor cells (CTCs) are cells that have shed into the vasculature from a primary tumor and circulate in the blood stream. Circulating tumor cells play an important role in metastasis. The long term goal of this project is to capture and remocve CTC’s from the blood stream. Numerous studies on glutamine metabolism in cancer indicate that many tumors are avid glutamine consumers in vivo and in vitro. Prolamins are a group of plant seed proteins having high glutamine content. A main example is corn zein. Zein has been reported to support cell attachment. The main hypothesis in this work was that zein is able to support attachment of cancer (HeLa) cells. In this work, HeLa cells were grown on glass slides coated with zein films. It was reported that the hydrophobicity and topography of substrates are important for cell attachment. Therefore, a secondary hypothesis was that the hydrophobicity and topography of zein substrates could be optimized for cancer cell attachment. The hydrophobicity of zein films had been observed to reflect the hydrophobicity of its underlying substrate. Thus, glass slides were treated with oxygen plasma to modulate their hydrophobicity. The surface hydrophobicity of substrates intended for cell attachment was measured with a CAM 200 Goniometer. Topographic features were examined by MFP-3D AFM. Results showed that HeLa cells were observed to adhere and grow on zein substrates cast on oxygen treated plasma glass slides. The glutamine effect of zein on HeLa cells was evidenced by the cells unfavorable response when zein was deamidated by surface treatment with hydrochloric acid. HeLa cells on zein films developed elongated cells at 24h and 48 h of incubation. Zein was believed to support HeLa cells attachment. Results of this work are expected to be useful in designing bio surfaces for isolation of cancer cells.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2015-09-29 without embargo terms","The student, Ying Qin, accepted the attached license on 2015-07-22 at 17:15.","The student, Ying Qin, submitted this Thesis for approval on 2015-07-22 at 17:20.","This Thesis was approved for publication on 2015-07-24 at 11:58.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8619 on 2015-09-29 at 13:23:36","Made available in DSpace on 2015-09-29T20:38:53Z (GMT). No. of bitstreams: 2 QIN-THESIS-2015.pdf: 3985433 bytes, checksum: d9a59de595d689d6309678993961d7fc (MD5) LICENSE.txt: 4205 bytes, checksum: 8cc38992c1e82fe40f067f53ca41224f (MD5) Previous issue date: 2015-07-24"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Zein films as substrates for cell attachment"]}]}],"canonical_facts":{"dc:contributor":["Padua, Graciela W."],"dc:creator":["Qin, Ying"],"dc:date":["2015-09-29T20:38:53Z","2015-08","2015-07-24","2015-8"],"dc:description":["Circulating tumor cells (CTCs) are cells that have shed into the vasculature from a primary tumor and circulate in the blood stream. Circulating tumor cells play an important role in metastasis. The long term goal of this project is to capture and remocve CTC’s from the blood stream. Numerous studies on glutamine metabolism in cancer indicate that many tumors are avid glutamine consumers in vivo and in vitro. Prolamins are a group of plant seed proteins having high glutamine content. A main example is corn zein. Zein has been reported to support cell attachment. The main hypothesis in this work was that zein is able to support attachment of cancer (HeLa) cells. In this work, HeLa cells were grown on glass slides coated with zein films. It was reported that the hydrophobicity and topography of substrates are important for cell attachment. Therefore, a secondary hypothesis was that the hydrophobicity and topography of zein substrates could be optimized for cancer cell attachment. The hydrophobicity of zein films had been observed to reflect the hydrophobicity of its underlying substrate. Thus, glass slides were treated with oxygen plasma to modulate their hydrophobicity. The surface hydrophobicity of substrates intended for cell attachment was measured with a CAM 200 Goniometer. Topographic features were examined by MFP-3D AFM. Results showed that HeLa cells were observed to adhere and grow on zein substrates cast on oxygen treated plasma glass slides. The glutamine effect of zein on HeLa cells was evidenced by the cells unfavorable response when zein was deamidated by surface treatment with hydrochloric acid. HeLa cells on zein films developed elongated cells at 24h and 48 h of incubation. Zein was believed to support HeLa cells attachment. Results of this work are expected to be useful in designing bio surfaces for isolation of cancer cells.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2015-09-29 without embargo terms","The student, Ying Qin, accepted the attached license on 2015-07-22 at 17:15.","The student, Ying Qin, submitted this Thesis for approval on 2015-07-22 at 17:20.","This Thesis was approved for publication on 2015-07-24 at 11:58.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8619 on 2015-09-29 at 13:23:36","Made available in DSpace on 2015-09-29T20:38:53Z (GMT). No. of bitstreams: 2 QIN-THESIS-2015.pdf: 3985433 bytes, checksum: d9a59de595d689d6309678993961d7fc (MD5) LICENSE.txt: 4205 bytes, checksum: 8cc38992c1e82fe40f067f53ca41224f (MD5) Previous issue date: 2015-07-24"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/88116"],"dc:language":["en"],"dc:rights":["Copyright 2015 Ying Qin"],"dc:subject":["zein films","HeLa cells"],"dc:title":["Zein films as substrates for cell attachment"],"dc:type":["text"],"thesis:degree_discipline":["Food Science & Human Nutrition"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:31Z"}