{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/87987"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/87987","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Biomolecularly and mechanically instructive materials for guiding cell-substrate interactions","abstract":"Techniques that enable the creation of instructive biomaterials have the potential to advance our understanding and ability to guide cell fate and function. Benzophenone (BP) photolithography is uniquely suited to generating chemically and mechanically tailored materials because of its molecularly general and spatially modulated characteristics. Here, we describe the application of this methodology to investigate two important biological processes in the context of mechanical and biochemical cues. First, we generated gradients of cellular adhesion molecules that allowed us to understand the mechanism by which bromelain affects cell rolling during the early steps of inflammation. The latter part of this work utilized BP-mediated immobilization on two substrates, collagen-glycosaminoglycan membranes and polyacrylamide hydrogels, to evaluate stem cell diffferentiation. In summary, we have developed a photoreactive platform that provides the capability to control biomolecule density separately from mechanical properties. This strategy can be used on a variety of biorelevant substrates to replicate the properties of the native extracellular matrix. Our results shed light on the combinatorial influence of multiple cues and have the potential to identify key parameters to specifically alter cell response with a view towards tissue regeneration.","abstract_html":"Techniques that enable the creation of instructive biomaterials have the potential to advance our understanding and ability to guide cell fate and function. Benzophenone (BP) photolithography is uniquely suited to generating chemically and mechanically tailored materials because of its molecularly general and spatially modulated characteristics. Here, we describe the application of this methodology to investigate two important biological processes in the context of mechanical and biochemical cues. First, we generated gradients of cellular adhesion molecules that allowed us to understand the mechanism by which bromelain affects cell rolling during the early steps of inflammation. The latter part of this work utilized BP-mediated immobilization on two substrates, collagen-glycosaminoglycan membranes and polyacrylamide hydrogels, to evaluate stem cell diffferentiation. In summary, we have developed a photoreactive platform that provides the capability to control biomolecule density separately from mechanical properties. This strategy can be used on a variety of biorelevant substrates to replicate the properties of the native extracellular matrix. Our results shed light on the combinatorial influence of multiple cues and have the potential to identify key parameters to specifically alter cell response with a view towards tissue regeneration.","abstract_has_math":false,"creators":["Banks, Jessica M"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Bailey, Ryan C.","Harley, Brendan A.","Mitchell, Douglas A.","Murphy, Catherine J."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-29T20:37:55Z","date_published":"2015-09-29T20:37:55Z","updated_at":"2026-07-22T22:26:31Z","subjects":["Stem cell differentiation","Biomaterials","Hydrogels","Collagen scaffolds","Leukocyte rolling","Photolithography","Benzophenone"],"languages":["en"],"rights":["Copyright 2015 Jessica Madeline Banks"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/87987","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Bailey, Ryan C.","Harley, Brendan A.","Mitchell, Douglas A.","Murphy, Catherine J."]},{"key":"dc:creator","label":"Author","values":["Banks, Jessica M"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-29T20:37:55Z","2015-08","2015-07-01","2015-8"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Stem cell differentiation","Biomaterials","Hydrogels","Collagen scaffolds","Leukocyte rolling","Photolithography","Benzophenone"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2015 Jessica Madeline Banks"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/87987"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Techniques that enable the creation of instructive biomaterials have the potential to advance our understanding and ability to guide cell fate and function. Benzophenone (BP) photolithography is uniquely suited to generating chemically and mechanically tailored materials because of its molecularly general and spatially modulated characteristics. Here, we describe the application of this methodology to investigate two important biological processes in the context of mechanical and biochemical cues. First, we generated gradients of cellular adhesion molecules that allowed us to understand the mechanism by which bromelain affects cell rolling during the early steps of inflammation. The latter part of this work utilized BP-mediated immobilization on two substrates, collagen-glycosaminoglycan membranes and polyacrylamide hydrogels, to evaluate stem cell diffferentiation. In summary, we have developed a photoreactive platform that provides the capability to control biomolecule density separately from mechanical properties. This strategy can be used on a variety of biorelevant substrates to replicate the properties of the native extracellular matrix. Our results shed light on the combinatorial influence of multiple cues and have the potential to identify key parameters to specifically alter cell response with a view towards tissue regeneration.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2015-09-29 without embargo terms","The student, Jessica Banks, accepted the attached license on 2015-06-30 at 12:56.","The student, Jessica Banks, submitted this Dissertation for approval on 2015-06-30 at 13:14.","This Dissertation was approved for publication on 2015-07-01 at 08:27.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8323 on 2015-09-29 at 13:21:44","Made available in DSpace on 2015-09-29T20:37:55Z (GMT). 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Benzophenone (BP) photolithography is uniquely suited to generating chemically and mechanically tailored materials because of its molecularly general and spatially modulated characteristics. Here, we describe the application of this methodology to investigate two important biological processes in the context of mechanical and biochemical cues. First, we generated gradients of cellular adhesion molecules that allowed us to understand the mechanism by which bromelain affects cell rolling during the early steps of inflammation. The latter part of this work utilized BP-mediated immobilization on two substrates, collagen-glycosaminoglycan membranes and polyacrylamide hydrogels, to evaluate stem cell diffferentiation. In summary, we have developed a photoreactive platform that provides the capability to control biomolecule density separately from mechanical properties. This strategy can be used on a variety of biorelevant substrates to replicate the properties of the native extracellular matrix. Our results shed light on the combinatorial influence of multiple cues and have the potential to identify key parameters to specifically alter cell response with a view towards tissue regeneration.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2015-09-29 without embargo terms","The student, Jessica Banks, accepted the attached license on 2015-06-30 at 12:56.","The student, Jessica Banks, submitted this Dissertation for approval on 2015-06-30 at 13:14.","This Dissertation was approved for publication on 2015-07-01 at 08:27.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8323 on 2015-09-29 at 13:21:44","Made available in DSpace on 2015-09-29T20:37:55Z (GMT). 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