{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/87913"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/87913","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Development & Application of Fluorescence Spectroscopy & Fluorescence Lifetime Imaging to Biology & Medicine","abstract":"The dissertation is concerned with biophysical applications of Forster resonance energy transfer (FRET) and development and application of fluorescence lifetime-resolved imaging microscopy (FLIM). It includes work to extend the FRET and FLIM methods for studying microsecond reaction kinetics. The common thread and unifying theme of the dissertation is the development and application of fluorescence spectroscopy and microscopy (in particular, FRET and FLIM) to biology and medicine. 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