{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/87828"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/87828","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The Use of Tissue Culture to Separate Apple (Malus Sp.) Chimeras Into Their Constituent Genotype(s) and to Induce Anthocyanin Production","abstract":"To assess somaclonal variation, 'Gala', 'Royal Gala' and 'Jonagold' trees obtained via axillary and adventitious bud formation were compared ex vitro to conventionally grafted trees. In general, tissue culture-derived trees were relatively erect and their branch angles were relatively narrower in comparison to grafted trees. All trees that flowered had pink blossoms and red to cream colored fruit. There were no obvious differences in flowering time or in floral morphology. Although there were differences in the range of fruit color and in the degree of pigmentation from tree-to-tree between 'Royal Gala' and 'Gala' apples for both control and tissue culture-derived plants, none of the variation exceeded that observed among control trees. We conclude that 'Gala' and 'Royal Gala' showed very little somaclonal variation for the morphological and reproductive traits we studied, suggesting tissue culture trees can be a good method to propagate apple plants.","abstract_html":"To assess somaclonal variation, &#x27;Gala&#x27;, &#x27;Royal Gala&#x27; and &#x27;Jonagold&#x27; trees obtained via axillary and adventitious bud formation were compared ex vitro to conventionally grafted trees. In general, tissue culture-derived trees were relatively erect and their branch angles were relatively narrower in comparison to grafted trees. All trees that flowered had pink blossoms and red to cream colored fruit. There were no obvious differences in flowering time or in floral morphology. Although there were differences in the range of fruit color and in the degree of pigmentation from tree-to-tree between &#x27;Royal Gala&#x27; and &#x27;Gala&#x27; apples for both control and tissue culture-derived plants, none of the variation exceeded that observed among control trees. We conclude that &#x27;Gala&#x27; and &#x27;Royal Gala&#x27; showed very little somaclonal variation for the morphological and reproductive traits we studied, suggesting tissue culture trees can be a good method to propagate apple plants.","abstract_has_math":false,"creators":["McMeans, Orlando Fitzgerald"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Horticulture","degree_department":null,"school":null,"contributors":["Skirvin, Robert M.","Girma Mitiku"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-28T16:59:29Z","date_published":"2015-09-28T16:59:29Z","updated_at":"2026-07-22T22:26:31Z","subjects":["Biology, Plant Physiology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI9737195"],"render_values":[{"text":"(MiAaPQ)AAI9737195","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/87828","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Skirvin, Robert M.","Girma Mitiku"]},{"key":"dc:creator","label":"Author","values":["McMeans, Orlando Fitzgerald"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-28T16:59:29Z","10000-01-01","1997"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Horticulture"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Plant Physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/87828","(MiAaPQ)AAI9737195"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["To assess somaclonal variation, 'Gala', 'Royal Gala' and 'Jonagold' trees obtained via axillary and adventitious bud formation were compared ex vitro to conventionally grafted trees. In general, tissue culture-derived trees were relatively erect and their branch angles were relatively narrower in comparison to grafted trees. All trees that flowered had pink blossoms and red to cream colored fruit. There were no obvious differences in flowering time or in floral morphology. Although there were differences in the range of fruit color and in the degree of pigmentation from tree-to-tree between 'Royal Gala' and 'Gala' apples for both control and tissue culture-derived plants, none of the variation exceeded that observed among control trees. We conclude that 'Gala' and 'Royal Gala' showed very little somaclonal variation for the morphological and reproductive traits we studied, suggesting tissue culture trees can be a good method to propagate apple plants.","Made available in DSpace on 2015-09-28T16:59:29Z (GMT). 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Chimeras Into Their Constituent Genotype(s) and to Induce Anthocyanin Production"]}]}],"canonical_facts":{"dc:contributor":["Skirvin, Robert M.","Girma Mitiku"],"dc:creator":["McMeans, Orlando Fitzgerald"],"dc:date":["2015-09-28T16:59:29Z","10000-01-01","1997"],"dc:description":["To assess somaclonal variation, 'Gala', 'Royal Gala' and 'Jonagold' trees obtained via axillary and adventitious bud formation were compared ex vitro to conventionally grafted trees. In general, tissue culture-derived trees were relatively erect and their branch angles were relatively narrower in comparison to grafted trees. All trees that flowered had pink blossoms and red to cream colored fruit. There were no obvious differences in flowering time or in floral morphology. Although there were differences in the range of fruit color and in the degree of pigmentation from tree-to-tree between 'Royal Gala' and 'Gala' apples for both control and tissue culture-derived plants, none of the variation exceeded that observed among control trees. We conclude that 'Gala' and 'Royal Gala' showed very little somaclonal variation for the morphological and reproductive traits we studied, suggesting tissue culture trees can be a good method to propagate apple plants.","Made available in DSpace on 2015-09-28T16:59:29Z (GMT). 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Chimeras Into Their Constituent Genotype(s) and to Induce Anthocyanin Production"],"dc:type":["text"],"thesis:degree_discipline":["Horticulture"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:31Z"}