{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/87631"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/87631","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Fatty Acid-Mediated Inhibition of Host Cell Invasion by Cryptosporidium Parvum","abstract":"We have purified from bovine colostrum digests an unsaturated fatty acid that inhibits Cryptosporidium parvum sporozoite invasion of host cells in vitro. A panel of similar lipids with varying chain lengths and degrees of saturation were tested for evidence of inhibitory activity in order to determine the range of specificity. We identified a subset of inhibitory fatty acids and investigated the structural characteristics required for anti-cryptosporidial activity. Finally, we examined potential mechanisms for the lipid-mediated inhibition of sporozoite-host cell adhesion and invasion in vitro. These mechanisms included direct cytotoxicity to host cells and/or parasites, interruption of normal signaling pathways and alterations in gene expression. Our assays showed that eighteen-carbon unsaturated and twenty-carbon polyunsaturated free fatty acids with free carboxyl ends inhibited the invasion of host enterocytes by sporozoites in vitro by blocking microneme secretion. These results indicate a potential chemotherapeutic utility for a subset of long chain unsaturated free fatty acids in the treatment of cryptosporidiosis.","abstract_html":"We have purified from bovine colostrum digests an unsaturated fatty acid that inhibits Cryptosporidium parvum sporozoite invasion of host cells in vitro. A panel of similar lipids with varying chain lengths and degrees of saturation were tested for evidence of inhibitory activity in order to determine the range of specificity. We identified a subset of inhibitory fatty acids and investigated the structural characteristics required for anti-cryptosporidial activity. Finally, we examined potential mechanisms for the lipid-mediated inhibition of sporozoite-host cell adhesion and invasion in vitro. These mechanisms included direct cytotoxicity to host cells and/or parasites, interruption of normal signaling pathways and alterations in gene expression. Our assays showed that eighteen-carbon unsaturated and twenty-carbon polyunsaturated free fatty acids with free carboxyl ends inhibited the invasion of host enterocytes by sporozoites in vitro by blocking microneme secretion. These results indicate a potential chemotherapeutic utility for a subset of long chain unsaturated free fatty acids in the treatment of cryptosporidiosis.","abstract_has_math":false,"creators":["Schmidt, Jo Ann"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Veterinary Pathobiology","degree_department":null,"school":null,"contributors":["Kuhlenschmidt, Mark S."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2005,"date_issued":"2005","date_published":"2005","updated_at":"2026-07-22T22:26:30Z","subjects":["Biology, Microbiology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3199135"],"render_values":[{"text":"(MiAaPQ)AAI3199135","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/87631","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kuhlenschmidt, Mark S."]},{"key":"dc:creator","label":"Author","values":["Schmidt, Jo Ann"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2005","2015-09-28T16:21:39Z","10000-01-01"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Veterinary Pathobiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Microbiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/87631","(MiAaPQ)AAI3199135"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["We have purified from bovine colostrum digests an unsaturated fatty acid that inhibits Cryptosporidium parvum sporozoite invasion of host cells in vitro. 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