{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/87273"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/87273","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Non-Invasive Detection of Anaerobic Metabolism in Exercising Muscle Using Proton MRI","abstract":"The second, and major, component of this thesis concerned the exercise-induced increases the 1H T2 of muscle water. The hypotheses were tested that the T2 increase results from increased intracellular volume (VI), decreased intracellular pH (pHI), and/or changes in magnetization transfer (MT) or other longitudinal relaxation mechanisms. Isolated frog gastrocnemii were treated with iodoacetate (IAA; to inhibit lactate production), NaCN (to maximize lactate production), or left untreated and then performed 60 1s/min maximal isometric contractions. The intracellular transverse relaxation time (T2,I increased more in NaCN-treated and untreated muscles than in IAA-treated muscles. The VI increase and pHI decrease were greater in NaCN-treated and untreated muscles than in IAA-treated muscles. There was no change in the MT ratio and a slight decrease in T1 following exercise. In separate experiments, V I and pHI were manipulated in non-exercising sartorii and quantitative relationships were developed between these variables and T 2,I. A multiple regression model shows that 62% of the variance in the T2,I change of exercise is accounted for by these two variables.","abstract_html":"The second, and major, component of this thesis concerned the exercise-induced increases the 1H T2 of muscle water. The hypotheses were tested that the T2 increase results from increased intracellular volume (VI), decreased intracellular pH (pHI), and/or changes in magnetization transfer (MT) or other longitudinal relaxation mechanisms. Isolated frog gastrocnemii were treated with iodoacetate (IAA; to inhibit lactate production), NaCN (to maximize lactate production), or left untreated and then performed 60 1s/min maximal isometric contractions. The intracellular transverse relaxation time (T2,I increased more in NaCN-treated and untreated muscles than in IAA-treated muscles. The VI increase and pHI decrease were greater in NaCN-treated and untreated muscles than in IAA-treated muscles. There was no change in the MT ratio and a slight decrease in T1 following exercise. In separate experiments, V I and pHI were manipulated in non-exercising sartorii and quantitative relationships were developed between these variables and T 2,I. A multiple regression model shows that 62% of the variance in the T2,I change of exercise is accounted for by these two variables.","abstract_has_math":false,"creators":["Damon, Bruce Murray"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Molecular and Integrative Physiology","degree_department":null,"school":null,"contributors":["Dawson, M. 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