{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/87231"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/87231","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The Pedunculopontine Nucleus: A Potential Site of Respiratory Modulation During Muscle Contraction and Hypoxia","abstract":"\"The studies contained in this dissertation begin to examine a potential role for the pedunculopontine nucleus (PPN) in the regulation of respiratory adjustments that accompany motor activity and hypoxia. The PPN, located in the dorsal mesencephalic tegmentum at the lateral extent of the brachium conjunctivum, has previously been implicated in regulation of cardiorespiratory adjustments that accompany physical activity through a feed-forward mechanism termed \"\"central command\"\". The primary goal of this dissertation was to address the hypothesis that the PPN mediates influences on reflex respiratory adjustments that accompany skeletal muscle contraction in anesthetized rats. Extracellular single-unit electrophysiologic recordings were employed in the first group of studies to demonstrate that individual PPN neurons exhibited increases in firing rate during activation of muscle reflex pathways during static muscle contraction. Activation of PPN neurons was shown to have functional significance in that microinjections of bicuculline, a GABAA receptor antagonist, into the PPN resulted in significant increases in respiratory activity. The second set of studies demonstrated importance of the integrity of pathways that synapse in the PPN to the respiratory responses observed during muscle contraction in that injections of CoCl2 into the PPN resulted in significant attenuation of the reflex diaphragmatic responses to muscle contraction. Finally, responses of PPN neurons were also examined during hypoxia-related stimuli. PPN neurons were found to exhibit increases in firing rate during systemic hypoxia in anesthetized adult rats. However, inhibitory responses during chemical hypoxia were documented amongst most PPN neurons recorded in brain slice preparations from neonatal rats. The results of the studies that comprise this dissertation suggest that the PPN may mediate influences on the respiratory system during physical activity and hypoxia and that the PPN warrants future attention to elucidate its role in the regulation of respiratory adjustments in these and other physiologic and pathophysiologic contexts.\"","abstract_html":""The studies contained in this dissertation begin to examine a potential role for the pedunculopontine nucleus (PPN) in the regulation of respiratory adjustments that accompany motor activity and hypoxia. The PPN, located in the dorsal mesencephalic tegmentum at the lateral extent of the brachium conjunctivum, has previously been implicated in regulation of cardiorespiratory adjustments that accompany physical activity through a feed-forward mechanism termed ""central command"". The primary goal of this dissertation was to address the hypothesis that the PPN mediates influences on reflex respiratory adjustments that accompany skeletal muscle contraction in anesthetized rats. Extracellular single-unit electrophysiologic recordings were employed in the first group of studies to demonstrate that individual PPN neurons exhibited increases in firing rate during activation of muscle reflex pathways during static muscle contraction. Activation of PPN neurons was shown to have functional significance in that microinjections of bicuculline, a GABAA receptor antagonist, into the PPN resulted in significant increases in respiratory activity. The second set of studies demonstrated importance of the integrity of pathways that synapse in the PPN to the respiratory responses observed during muscle contraction in that injections of CoCl2 into the PPN resulted in significant attenuation of the reflex diaphragmatic responses to muscle contraction. Finally, responses of PPN neurons were also examined during hypoxia-related stimuli. PPN neurons were found to exhibit increases in firing rate during systemic hypoxia in anesthetized adult rats. However, inhibitory responses during chemical hypoxia were documented amongst most PPN neurons recorded in brain slice preparations from neonatal rats. The results of the studies that comprise this dissertation suggest that the PPN may mediate influences on the respiratory system during physical activity and hypoxia and that the PPN warrants future attention to elucidate its role in the regulation of respiratory adjustments in these and other physiologic and pathophysiologic contexts."","abstract_has_math":false,"creators":["Plowey, Edward Douglas"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Molecular and Integrative Physiology","degree_department":null,"school":null,"contributors":["Waldrop, Tony G."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-28T15:50:08Z","date_published":"2015-09-28T15:50:08Z","updated_at":"2026-07-22T22:26:28Z","subjects":["Biology, Neuroscience"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3153401"],"render_values":[{"text":"(MiAaPQ)AAI3153401","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/87231","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Waldrop, Tony G."]},{"key":"dc:creator","label":"Author","values":["Plowey, Edward Douglas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-28T15:50:08Z","10000-01-01","2004"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Molecular and Integrative Physiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Neuroscience"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/87231","(MiAaPQ)AAI3153401"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["\"The studies contained in this dissertation begin to examine a potential role for the pedunculopontine nucleus (PPN) in the regulation of respiratory adjustments that accompany motor activity and hypoxia. The PPN, located in the dorsal mesencephalic tegmentum at the lateral extent of the brachium conjunctivum, has previously been implicated in regulation of cardiorespiratory adjustments that accompany physical activity through a feed-forward mechanism termed \"\"central command\"\". The primary goal of this dissertation was to address the hypothesis that the PPN mediates influences on reflex respiratory adjustments that accompany skeletal muscle contraction in anesthetized rats. Extracellular single-unit electrophysiologic recordings were employed in the first group of studies to demonstrate that individual PPN neurons exhibited increases in firing rate during activation of muscle reflex pathways during static muscle contraction. Activation of PPN neurons was shown to have functional significance in that microinjections of bicuculline, a GABAA receptor antagonist, into the PPN resulted in significant increases in respiratory activity. The second set of studies demonstrated importance of the integrity of pathways that synapse in the PPN to the respiratory responses observed during muscle contraction in that injections of CoCl2 into the PPN resulted in significant attenuation of the reflex diaphragmatic responses to muscle contraction. Finally, responses of PPN neurons were also examined during hypoxia-related stimuli. PPN neurons were found to exhibit increases in firing rate during systemic hypoxia in anesthetized adult rats. However, inhibitory responses during chemical hypoxia were documented amongst most PPN neurons recorded in brain slice preparations from neonatal rats. The results of the studies that comprise this dissertation suggest that the PPN may mediate influences on the respiratory system during physical activity and hypoxia and that the PPN warrants future attention to elucidate its role in the regulation of respiratory adjustments in these and other physiologic and pathophysiologic contexts.\"","Made available in DSpace on 2015-09-28T15:50:08Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3153401.pdf: 11398932 bytes, checksum: 46ef0b4f697c10f3908c670662a49a47 (MD5) Previous issue date: 2004","Embargo set by: Seth Robbins for item 88512 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","139 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2004."]},{"key":"dc:title","label":"Title","values":["The Pedunculopontine Nucleus: A Potential Site of Respiratory Modulation During Muscle Contraction and Hypoxia"]}]}],"canonical_facts":{"dc:contributor":["Waldrop, Tony G."],"dc:creator":["Plowey, Edward Douglas"],"dc:date":["2015-09-28T15:50:08Z","10000-01-01","2004"],"dc:description":["\"The studies contained in this dissertation begin to examine a potential role for the pedunculopontine nucleus (PPN) in the regulation of respiratory adjustments that accompany motor activity and hypoxia. The PPN, located in the dorsal mesencephalic tegmentum at the lateral extent of the brachium conjunctivum, has previously been implicated in regulation of cardiorespiratory adjustments that accompany physical activity through a feed-forward mechanism termed \"\"central command\"\". The primary goal of this dissertation was to address the hypothesis that the PPN mediates influences on reflex respiratory adjustments that accompany skeletal muscle contraction in anesthetized rats. Extracellular single-unit electrophysiologic recordings were employed in the first group of studies to demonstrate that individual PPN neurons exhibited increases in firing rate during activation of muscle reflex pathways during static muscle contraction. Activation of PPN neurons was shown to have functional significance in that microinjections of bicuculline, a GABAA receptor antagonist, into the PPN resulted in significant increases in respiratory activity. The second set of studies demonstrated importance of the integrity of pathways that synapse in the PPN to the respiratory responses observed during muscle contraction in that injections of CoCl2 into the PPN resulted in significant attenuation of the reflex diaphragmatic responses to muscle contraction. Finally, responses of PPN neurons were also examined during hypoxia-related stimuli. PPN neurons were found to exhibit increases in firing rate during systemic hypoxia in anesthetized adult rats. However, inhibitory responses during chemical hypoxia were documented amongst most PPN neurons recorded in brain slice preparations from neonatal rats. The results of the studies that comprise this dissertation suggest that the PPN may mediate influences on the respiratory system during physical activity and hypoxia and that the PPN warrants future attention to elucidate its role in the regulation of respiratory adjustments in these and other physiologic and pathophysiologic contexts.\"","Made available in DSpace on 2015-09-28T15:50:08Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3153401.pdf: 11398932 bytes, checksum: 46ef0b4f697c10f3908c670662a49a47 (MD5) Previous issue date: 2004","Embargo set by: Seth Robbins for item 88512 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","139 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2004."],"dc:identifier":["http://hdl.handle.net/2142/87231","(MiAaPQ)AAI3153401"],"dc:language":["eng"],"dc:subject":["Biology, Neuroscience"],"dc:title":["The Pedunculopontine Nucleus: A Potential Site of Respiratory Modulation During Muscle Contraction and Hypoxia"],"dc:type":["text"],"thesis:degree_discipline":["Molecular and Integrative Physiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:28Z"}