{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/87212"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/87212","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Cholinergic Regulation of Circadian Rhythms in the SCN: Characterization of Signaling Mechanisms and the Role of Intracellular Calcium in the Directionality of Clock Resetting","abstract":"This study shows that stimulus-specific intracellular Ca2+ (Ca2+i) release controls the direction of clock resetting. Direct activation of the InsP3-induced Ca2+ i release (IICR) mimics cholinergic phase advance, while InsP3 , but not ryanodine, receptor antagonists block it. In contrast, selective activation of CICR with ryanodine results in glutamate-like delay. Ca 2+ imaging using multiphoton microscopy revealed signal-specific differences in Ca2+ transients. Cholinergic-stimulated Ca2+ transients are localized to the microdomains in the cytoplasm, whereas glutamate-induced Ca2+ transients are predominantly uniform across the cytosol and nucleus. Thus, the direction of clock resetting is determined by differential Ca2+i release that is likely to result in activation of spatially restricted Ca2+-dependent effector proteins.","abstract_html":"This study shows that stimulus-specific intracellular Ca2+ (Ca2+i) release controls the direction of clock resetting. Direct activation of the InsP3-induced Ca2+ i release (IICR) mimics cholinergic phase advance, while InsP3 , but not ryanodine, receptor antagonists block it. In contrast, selective activation of CICR with ryanodine results in glutamate-like delay. Ca 2+ imaging using multiphoton microscopy revealed signal-specific differences in Ca2+ transients. Cholinergic-stimulated Ca2+ transients are localized to the microdomains in the cytoplasm, whereas glutamate-induced Ca2+ transients are predominantly uniform across the cytosol and nucleus. Thus, the direction of clock resetting is determined by differential Ca2+i release that is likely to result in activation of spatially restricted Ca2+-dependent effector proteins.","abstract_has_math":false,"creators":["Artinian, Liana"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Molecular and Integrative Physiology","degree_department":null,"school":null,"contributors":["Gillette, Martha U."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-28T15:50:01Z","date_published":"2015-09-28T15:50:01Z","updated_at":"2026-07-22T22:26:28Z","subjects":["Biology, Neuroscience"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3017015"],"render_values":[{"text":"(MiAaPQ)AAI3017015","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/87212","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Gillette, Martha U."]},{"key":"dc:creator","label":"Author","values":["Artinian, Liana"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-28T15:50:01Z","10000-01-01","2001"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Molecular and Integrative Physiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Neuroscience"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/87212","(MiAaPQ)AAI3017015"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["This study shows that stimulus-specific intracellular Ca2+ (Ca2+i) release controls the direction of clock resetting. Direct activation of the InsP3-induced Ca2+ i release (IICR) mimics cholinergic phase advance, while InsP3 , but not ryanodine, receptor antagonists block it. In contrast, selective activation of CICR with ryanodine results in glutamate-like delay. Ca 2+ imaging using multiphoton microscopy revealed signal-specific differences in Ca2+ transients. Cholinergic-stimulated Ca2+ transients are localized to the microdomains in the cytoplasm, whereas glutamate-induced Ca2+ transients are predominantly uniform across the cytosol and nucleus. Thus, the direction of clock resetting is determined by differential Ca2+i release that is likely to result in activation of spatially restricted Ca2+-dependent effector proteins.","Made available in DSpace on 2015-09-28T15:50:01Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3017015.pdf: 6345951 bytes, checksum: 82fd26994e0c1aae16db53361f5f0f5b (MD5) Previous issue date: 2001","Embargo set by: Seth Robbins for item 88493 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","120 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2001."]},{"key":"dc:title","label":"Title","values":["Cholinergic Regulation of Circadian Rhythms in the SCN: Characterization of Signaling Mechanisms and the Role of Intracellular Calcium in the Directionality of Clock Resetting"]}]}],"canonical_facts":{"dc:contributor":["Gillette, Martha U."],"dc:creator":["Artinian, Liana"],"dc:date":["2015-09-28T15:50:01Z","10000-01-01","2001"],"dc:description":["This study shows that stimulus-specific intracellular Ca2+ (Ca2+i) release controls the direction of clock resetting. Direct activation of the InsP3-induced Ca2+ i release (IICR) mimics cholinergic phase advance, while InsP3 , but not ryanodine, receptor antagonists block it. In contrast, selective activation of CICR with ryanodine results in glutamate-like delay. Ca 2+ imaging using multiphoton microscopy revealed signal-specific differences in Ca2+ transients. Cholinergic-stimulated Ca2+ transients are localized to the microdomains in the cytoplasm, whereas glutamate-induced Ca2+ transients are predominantly uniform across the cytosol and nucleus. Thus, the direction of clock resetting is determined by differential Ca2+i release that is likely to result in activation of spatially restricted Ca2+-dependent effector proteins.","Made available in DSpace on 2015-09-28T15:50:01Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3017015.pdf: 6345951 bytes, checksum: 82fd26994e0c1aae16db53361f5f0f5b (MD5) Previous issue date: 2001","Embargo set by: Seth Robbins for item 88493 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","120 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2001."],"dc:identifier":["http://hdl.handle.net/2142/87212","(MiAaPQ)AAI3017015"],"dc:language":["eng"],"dc:subject":["Biology, Neuroscience"],"dc:title":["Cholinergic Regulation of Circadian Rhythms in the SCN: Characterization of Signaling Mechanisms and the Role of Intracellular Calcium in the Directionality of Clock Resetting"],"dc:type":["text"],"thesis:degree_discipline":["Molecular and Integrative Physiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:28Z"}