{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/86766"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/86766","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Total Genomic Transcriptional Analysis of the Fatty Acid Degradation Regulon of Escherichia Coli","abstract":"The second ORF, b2323 is the fabB gene which produces beta-ketoacyl-ACP synthase I. This is one of two long chain specific condensing enzymes in E. coli and is required for unsaturated fatty acid biosynthesis. The other enzyme necessary for producing unsaturated fatty acids is 3-hydroxy-decanoyl-ACP dehydrase, encoded by the fabA gene. This gene has previously been shown to be positively regulated by FadR. Double fabA(Ts), fadR mutants have a synthetic lethal phenotype, fabB(Ts), fadR strains were constructed and shown to behave in a similar fashion. Experiments involving chloramphenicol acetyl transferase fusions confirm that FadR positively regulates transcription of fabB as well.","abstract_html":"The second ORF, b2323 is the fabB gene which produces beta-ketoacyl-ACP synthase I. This is one of two long chain specific condensing enzymes in E. coli and is required for unsaturated fatty acid biosynthesis. The other enzyme necessary for producing unsaturated fatty acids is 3-hydroxy-decanoyl-ACP dehydrase, encoded by the fabA gene. This gene has previously been shown to be positively regulated by FadR. Double fabA(Ts), fadR mutants have a synthetic lethal phenotype, fabB(Ts), fadR strains were constructed and shown to behave in a similar fashion. Experiments involving chloramphenicol acetyl transferase fusions confirm that FadR positively regulates transcription of fabB as well.","abstract_has_math":false,"creators":["Campbell, John Walter"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Cronan, John E., Jr"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-28T15:17:56Z","date_published":"2015-09-28T15:17:56Z","updated_at":"2026-07-22T22:26:27Z","subjects":["Biology, Genetics"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI9996620"],"render_values":[{"text":"(MiAaPQ)AAI9996620","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/86766","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Cronan, John E., Jr"]},{"key":"dc:creator","label":"Author","values":["Campbell, John Walter"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-28T15:17:56Z","10000-01-01","2001"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Genetics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/86766","(MiAaPQ)AAI9996620"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The second ORF, b2323 is the fabB gene which produces beta-ketoacyl-ACP synthase I. This is one of two long chain specific condensing enzymes in E. coli and is required for unsaturated fatty acid biosynthesis. The other enzyme necessary for producing unsaturated fatty acids is 3-hydroxy-decanoyl-ACP dehydrase, encoded by the fabA gene. This gene has previously been shown to be positively regulated by FadR. Double fabA(Ts), fadR mutants have a synthetic lethal phenotype, fabB(Ts), fadR strains were constructed and shown to behave in a similar fashion. Experiments involving chloramphenicol acetyl transferase fusions confirm that FadR positively regulates transcription of fabB as well.","Made available in DSpace on 2015-09-28T15:17:56Z (GMT). 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This is one of two long chain specific condensing enzymes in E. coli and is required for unsaturated fatty acid biosynthesis. The other enzyme necessary for producing unsaturated fatty acids is 3-hydroxy-decanoyl-ACP dehydrase, encoded by the fabA gene. This gene has previously been shown to be positively regulated by FadR. Double fabA(Ts), fadR mutants have a synthetic lethal phenotype, fabB(Ts), fadR strains were constructed and shown to behave in a similar fashion. Experiments involving chloramphenicol acetyl transferase fusions confirm that FadR positively regulates transcription of fabB as well.","Made available in DSpace on 2015-09-28T15:17:56Z (GMT). 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