Functional Analysis of the N -Terminal Transmembrane/signal -Anchor Sequence and Linker Domain of Cytochrome P450 2C2
type: CreativeWork
description: Truncated forms of P450 2C2 lacking the N-terminal hydrophobic signal anchor sequence (Delta2- 20) or the signal anchor and linker region (Delta2-27) were expressed alone or as GFP fusions in E. coli and insect cells. The findings indicate that deletion of the signal anchor decreases assembly of P450 hemoprotein in E. coli but does not change the in vivo distribution of the protein. However, it alters the nature of membrane interaction such that P450 2C2 is no longer an integral membrane protein. Moreover, the results show that the linker domain is critical for the formation of functional P450 2C2 only when the P450 molecule is properly targeted and inserted in the membrane.
abstract: Truncated forms of P450 2C2 lacking the N-terminal hydrophobic signal anchor sequence (Delta2- 20) or the signal anchor and linker region (Delta2-27) were expressed alone or as GFP fusions in E. coli and insect cells. The findings indicate that deletion of the signal anchor decreases assembly of P450 hemoprotein in E. coli but does not change the in vivo distribution of the protein. However, it alters the nature of membrane interaction such that P450 2C2 is no longer an integral membrane protein. Moreover, the results show that the linker domain is critical for the formation of functional P450 2C2 only when the P450 molecule is properly targeted and inserted in the membrane.
author: D., Balraj
language: eng
keywords: Biology, Molecular
date published: 2015-09-28T15:17:50Z
date modified: 2026-07-22T22:26:27Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/86753