{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/86752"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/86752","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Proline Utilization in Salmonella Typhimurium: A Model for Regulation of Peripheral-Membrane Flavin Dehydrogenase-Lipid Interactions","abstract":"When it is located in the cytoplasm, PutA exists as a soluble dimer that is available to bind put operator DNA. However, proline oxidation and available membrane lipid allow PutA to undergo a dimer-to-monomer transition that is required for membrane association. On the other hand, if membrane lipid is not readily available PutA remains in the soluble, dimeric conformation and represses transcription of the put operon. Thus, although both proline and membrane lipid are required for induction of the put operon, a quaternary transition appears critical to prevent overexpression toxicity by this peripheral membrane flavin dehydrogenase.","abstract_html":"When it is located in the cytoplasm, PutA exists as a soluble dimer that is available to bind put operator DNA. However, proline oxidation and available membrane lipid allow PutA to undergo a dimer-to-monomer transition that is required for membrane association. On the other hand, if membrane lipid is not readily available PutA remains in the soluble, dimeric conformation and represses transcription of the put operon. Thus, although both proline and membrane lipid are required for induction of the put operon, a quaternary transition appears critical to prevent overexpression toxicity by this peripheral membrane flavin dehydrogenase.","abstract_has_math":false,"creators":["Surber, Mark William"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Stanley Maloy"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-28T15:17:50Z","date_published":"2015-09-28T15:17:50Z","updated_at":"2026-07-22T22:26:27Z","subjects":["Biology, Genetics"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI9953152"],"render_values":[{"text":"(MiAaPQ)AAI9953152","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/86752","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Stanley Maloy"]},{"key":"dc:creator","label":"Author","values":["Surber, Mark William"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-28T15:17:50Z","10000-01-01","1999"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Genetics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/86752","(MiAaPQ)AAI9953152"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["When it is located in the cytoplasm, PutA exists as a soluble dimer that is available to bind put operator DNA. However, proline oxidation and available membrane lipid allow PutA to undergo a dimer-to-monomer transition that is required for membrane association. On the other hand, if membrane lipid is not readily available PutA remains in the soluble, dimeric conformation and represses transcription of the put operon. Thus, although both proline and membrane lipid are required for induction of the put operon, a quaternary transition appears critical to prevent overexpression toxicity by this peripheral membrane flavin dehydrogenase.","Made available in DSpace on 2015-09-28T15:17:50Z (GMT). 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However, proline oxidation and available membrane lipid allow PutA to undergo a dimer-to-monomer transition that is required for membrane association. On the other hand, if membrane lipid is not readily available PutA remains in the soluble, dimeric conformation and represses transcription of the put operon. Thus, although both proline and membrane lipid are required for induction of the put operon, a quaternary transition appears critical to prevent overexpression toxicity by this peripheral membrane flavin dehydrogenase.","Made available in DSpace on 2015-09-28T15:17:50Z (GMT). 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