{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/86739"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/86739","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Fatty Acid and Biotin Metabolism in Escherichia Coli","abstract":"Biotin which is essential for the synthesis of fatty acids in E. coli is thought to be derived from a modified fatty acid pathway. In experiments used to verify this hypothesis I discovered a new intermediate in biotin synthesis. This intermediate `X', was purified from the spent growth medium of strain sup151 (isolated as a bypass suppressor of a bioH mutation). Genetic data suggests that this intermediate is made by the action of the bioH and bioC gene products and its production is repressed by high levels of biotin in the growth medium via the BirA protein.","abstract_html":"Biotin which is essential for the synthesis of fatty acids in E. coli is thought to be derived from a modified fatty acid pathway. In experiments used to verify this hypothesis I discovered a new intermediate in biotin synthesis. This intermediate `X', was purified from the spent growth medium of strain sup151 (isolated as a bypass suppressor of a bioH mutation). Genetic data suggests that this intermediate is made by the action of the bioH and bioC gene products and its production is repressed by high levels of biotin in the growth medium via the BirA protein.","abstract_has_math":false,"creators":["Subrahmanyam, Satyanarayana"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Cronan, John E., Jr"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-28T15:17:45Z","date_published":"2015-09-28T15:17:45Z","updated_at":"2026-07-22T22:26:27Z","subjects":["Chemistry, Biochemistry"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI9912390"],"render_values":[{"text":"(MiAaPQ)AAI9912390","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/86739","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Cronan, John E., Jr"]},{"key":"dc:creator","label":"Author","values":["Subrahmanyam, Satyanarayana"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-28T15:17:45Z","10000-01-01","1998"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/86739","(MiAaPQ)AAI9912390"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Biotin which is essential for the synthesis of fatty acids in E. coli is thought to be derived from a modified fatty acid pathway. In experiments used to verify this hypothesis I discovered a new intermediate in biotin synthesis. This intermediate `X', was purified from the spent growth medium of strain sup151 (isolated as a bypass suppressor of a bioH mutation). Genetic data suggests that this intermediate is made by the action of the bioH and bioC gene products and its production is repressed by high levels of biotin in the growth medium via the BirA protein.","Made available in DSpace on 2015-09-28T15:17:45Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9912390.pdf: 3958500 bytes, checksum: ef7c3b9d48faa6251f02e17a3fab572f (MD5) Previous issue date: 1998","Embargo set by: Seth Robbins for item 88020 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","176 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998."]},{"key":"dc:title","label":"Title","values":["Fatty Acid and Biotin Metabolism in Escherichia Coli"]}]}],"canonical_facts":{"dc:contributor":["Cronan, John E., Jr"],"dc:creator":["Subrahmanyam, Satyanarayana"],"dc:date":["2015-09-28T15:17:45Z","10000-01-01","1998"],"dc:description":["Biotin which is essential for the synthesis of fatty acids in E. coli is thought to be derived from a modified fatty acid pathway. In experiments used to verify this hypothesis I discovered a new intermediate in biotin synthesis. This intermediate `X', was purified from the spent growth medium of strain sup151 (isolated as a bypass suppressor of a bioH mutation). Genetic data suggests that this intermediate is made by the action of the bioH and bioC gene products and its production is repressed by high levels of biotin in the growth medium via the BirA protein.","Made available in DSpace on 2015-09-28T15:17:45Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9912390.pdf: 3958500 bytes, checksum: ef7c3b9d48faa6251f02e17a3fab572f (MD5) Previous issue date: 1998","Embargo set by: Seth Robbins for item 88020 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","176 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998."],"dc:identifier":["http://hdl.handle.net/2142/86739","(MiAaPQ)AAI9912390"],"dc:language":["eng"],"dc:subject":["Chemistry, Biochemistry"],"dc:title":["Fatty Acid and Biotin Metabolism in Escherichia Coli"],"dc:type":["text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:27Z"}