{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/86730"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/86730","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Elucidation of a Macrophage Cell Surface Receptor With Specificity for Exogenous Aromatic Hapten-Protein Antigens and Effects on Antigen Uptake and Processing","abstract":"Quantitative analyses of the binding interactions demonstrated that the kinetics of antigen uptake was dependent on the extent of receptor crosslinkage at the cell surface as well as on the accessibility of the ligand groups. Determination of the ligand-binding specificity of the macrophage receptor suggested preference for aromatic ligands like phenylalanine, 2-phenyloxazolone and fluorescein. Biochemical purification and partial characterization of the putative receptor were undertaken as one of the major goals of this thesis. The experiments described herein are the first to convincingly demonstrate the existence of an aromatic hapten-binding plasma membrane protein on macrophage cells with specificity for fluorescein. Moreover, the models proposed in this thesis have helped the further elucidation of the finer principles that dictate exogenous antigen processing and presentation in macrophage cells.","abstract_html":"Quantitative analyses of the binding interactions demonstrated that the kinetics of antigen uptake was dependent on the extent of receptor crosslinkage at the cell surface as well as on the accessibility of the ligand groups. Determination of the ligand-binding specificity of the macrophage receptor suggested preference for aromatic ligands like phenylalanine, 2-phenyloxazolone and fluorescein. Biochemical purification and partial characterization of the putative receptor were undertaken as one of the major goals of this thesis. The experiments described herein are the first to convincingly demonstrate the existence of an aromatic hapten-binding plasma membrane protein on macrophage cells with specificity for fluorescein. Moreover, the models proposed in this thesis have helped the further elucidation of the finer principles that dictate exogenous antigen processing and presentation in macrophage cells.","abstract_has_math":false,"creators":["Cherukuri, Anupama"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Voss, Edward W., Jr."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1998,"date_issued":"1998","date_published":"1998","updated_at":"2026-07-22T22:26:27Z","subjects":["Health Sciences, Immunology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI9904408"],"render_values":[{"text":"(MiAaPQ)AAI9904408","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/86730","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Voss, Edward W., Jr."]},{"key":"dc:creator","label":"Author","values":["Cherukuri, Anupama"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["1998","2015-09-28T15:17:40Z","10000-01-01"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Health Sciences, Immunology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/86730","(MiAaPQ)AAI9904408"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Quantitative analyses of the binding interactions demonstrated that the kinetics of antigen uptake was dependent on the extent of receptor crosslinkage at the cell surface as well as on the accessibility of the ligand groups. Determination of the ligand-binding specificity of the macrophage receptor suggested preference for aromatic ligands like phenylalanine, 2-phenyloxazolone and fluorescein. Biochemical purification and partial characterization of the putative receptor were undertaken as one of the major goals of this thesis. The experiments described herein are the first to convincingly demonstrate the existence of an aromatic hapten-binding plasma membrane protein on macrophage cells with specificity for fluorescein. Moreover, the models proposed in this thesis have helped the further elucidation of the finer principles that dictate exogenous antigen processing and presentation in macrophage cells.","Made available in DSpace on 2015-09-28T15:17:40Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9904408.pdf: 9952903 bytes, checksum: 99aed7a499e355b7cb6e9810d19995f1 (MD5) Previous issue date: 1998","Embargo set by: Seth Robbins for item 88011 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","275 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998."]},{"key":"dc:title","label":"Title","values":["Elucidation of a Macrophage Cell Surface Receptor With Specificity for Exogenous Aromatic Hapten-Protein Antigens and Effects on Antigen Uptake and Processing"]}]}],"canonical_facts":{"dc:contributor":["Voss, Edward W., Jr."],"dc:creator":["Cherukuri, Anupama"],"dc:date":["1998","2015-09-28T15:17:40Z","10000-01-01"],"dc:description":["Quantitative analyses of the binding interactions demonstrated that the kinetics of antigen uptake was dependent on the extent of receptor crosslinkage at the cell surface as well as on the accessibility of the ligand groups. Determination of the ligand-binding specificity of the macrophage receptor suggested preference for aromatic ligands like phenylalanine, 2-phenyloxazolone and fluorescein. Biochemical purification and partial characterization of the putative receptor were undertaken as one of the major goals of this thesis. The experiments described herein are the first to convincingly demonstrate the existence of an aromatic hapten-binding plasma membrane protein on macrophage cells with specificity for fluorescein. Moreover, the models proposed in this thesis have helped the further elucidation of the finer principles that dictate exogenous antigen processing and presentation in macrophage cells.","Made available in DSpace on 2015-09-28T15:17:40Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9904408.pdf: 9952903 bytes, checksum: 99aed7a499e355b7cb6e9810d19995f1 (MD5) Previous issue date: 1998","Embargo set by: Seth Robbins for item 88011 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","275 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998."],"dc:identifier":["http://hdl.handle.net/2142/86730","(MiAaPQ)AAI9904408"],"dc:language":["eng"],"dc:subject":["Health Sciences, Immunology"],"dc:title":["Elucidation of a Macrophage Cell Surface Receptor With Specificity for Exogenous Aromatic Hapten-Protein Antigens and Effects on Antigen Uptake and Processing"],"dc:type":["text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:27Z"}