{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/86727"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/86727","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Oxygen Regulated Genes in Salmonella Typhimurium","abstract":"Studies on oxd-2 showed that it encodes a 42-kD protein which has acetate/propionate kinase activities. The cloned oxd-2 gene, under a foreign promoter, complemented an ackA (encoding an acetate kinase) mutation. Both Oxd-2 and AckA have a similar substrate range, and Oxd-2 has higher affinity for propionate than for acetate. Transcription of oxd-2 was induced to high levels during anaerobic growth in an amino acid rich medium. The highest level was found in cells grown anaerobically in a medium containing serine as a carbon source and supplemented with aspartate, leucine, and threonine. oxd-2 expression was affected by mutations in fnr, hip (IHF), crp, lrp, ackA or pta. NMR analysis of the cultures of a wildtype strain, grown anaerobically in a medium containing threonine, aspartate, leucine and casamino acids, showed that S. typhimurium produced propionate from threonine.","abstract_html":"Studies on oxd-2 showed that it encodes a 42-kD protein which has acetate/propionate kinase activities. The cloned oxd-2 gene, under a foreign promoter, complemented an ackA (encoding an acetate kinase) mutation. Both Oxd-2 and AckA have a similar substrate range, and Oxd-2 has higher affinity for propionate than for acetate. Transcription of oxd-2 was induced to high levels during anaerobic growth in an amino acid rich medium. The highest level was found in cells grown anaerobically in a medium containing serine as a carbon source and supplemented with aspartate, leucine, and threonine. oxd-2 expression was affected by mutations in fnr, hip (IHF), crp, lrp, ackA or pta. NMR analysis of the cultures of a wildtype strain, grown anaerobically in a medium containing threonine, aspartate, leucine and casamino acids, showed that S. typhimurium produced propionate from threonine.","abstract_has_math":false,"creators":["Wei, Yan"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Miller, Charles G."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-28T15:17:39Z","date_published":"2015-09-28T15:17:39Z","updated_at":"2026-07-22T22:26:27Z","subjects":["Biology, Molecular"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI9812805"],"render_values":[{"text":"(MiAaPQ)AAI9812805","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/86727","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Miller, Charles G."]},{"key":"dc:creator","label":"Author","values":["Wei, Yan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-28T15:17:39Z","10000-01-01","1997"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/86727","(MiAaPQ)AAI9812805"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Studies on oxd-2 showed that it encodes a 42-kD protein which has acetate/propionate kinase activities. The cloned oxd-2 gene, under a foreign promoter, complemented an ackA (encoding an acetate kinase) mutation. Both Oxd-2 and AckA have a similar substrate range, and Oxd-2 has higher affinity for propionate than for acetate. Transcription of oxd-2 was induced to high levels during anaerobic growth in an amino acid rich medium. The highest level was found in cells grown anaerobically in a medium containing serine as a carbon source and supplemented with aspartate, leucine, and threonine. oxd-2 expression was affected by mutations in fnr, hip (IHF), crp, lrp, ackA or pta. NMR analysis of the cultures of a wildtype strain, grown anaerobically in a medium containing threonine, aspartate, leucine and casamino acids, showed that S. typhimurium produced propionate from threonine.","Made available in DSpace on 2015-09-28T15:17:39Z (GMT). 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The cloned oxd-2 gene, under a foreign promoter, complemented an ackA (encoding an acetate kinase) mutation. Both Oxd-2 and AckA have a similar substrate range, and Oxd-2 has higher affinity for propionate than for acetate. Transcription of oxd-2 was induced to high levels during anaerobic growth in an amino acid rich medium. The highest level was found in cells grown anaerobically in a medium containing serine as a carbon source and supplemented with aspartate, leucine, and threonine. oxd-2 expression was affected by mutations in fnr, hip (IHF), crp, lrp, ackA or pta. NMR analysis of the cultures of a wildtype strain, grown anaerobically in a medium containing threonine, aspartate, leucine and casamino acids, showed that S. typhimurium produced propionate from threonine.","Made available in DSpace on 2015-09-28T15:17:39Z (GMT). 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