Engineering T Cell Receptors to Examine the Role of Affinity and *Kinetics in T Helper Cell Function
type: CreativeWork
description: Chapter five details the engineering of a class II restricted human TCR isolated from a patient with multiple sclerosis called 3A6. Yeast display of TCRs has previously relied on the use of an antibody specific for a properly folded TCR, here various strategies were used to generate stabilized 3A6 TCRs. Helpful strategies included: alteration of error-prone PCR dNTP concentrations, increasing induction media galactose concentrations, and generation of three-domain TCR constructs (Valpha-VbetaCbeta).
abstract: Chapter five details the engineering of a class II restricted human TCR isolated from a patient with multiple sclerosis called 3A6. Yeast display of TCRs has previously relied on the use of an antibody specific for a properly folded TCR, here various strategies were used to generate stabilized 3A6 TCRs. Helpful strategies included: alteration of error-prone PCR dNTP concentrations, increasing induction media galactose concentrations, and generation of three-domain TCR constructs (Valpha-VbetaCbeta).
author: Weber, K. Scott
language: eng
keywords: Health Sciences, Immunology
date published: 2015-09-28T15:17:24Z
date modified: 2026-07-22T22:26:27Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/86681