{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/86642"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/86642","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Identification, Characterization and Mechanism of the Estrogen Regulation of the Gene Encoding the Sodium /Hydrogen Exchanger -Regulatory Factor (nhe-Rf)","abstract":"\"Finally, studies were designed to help define the physiological role of estrogen-induction of the NHE-RF gene using two model systems. Immunocytochemistry was used to visualize NHE-RF in MCF-7 cells. Secondly, we created adenovirus that express the wild type or anti-sense NHE-RF RNA. By infecting cells with the adenovirus we may overexpress or functionally \"\"knock out\"\" the NHE-RF protein and monitor the resulting effects on cell signaling, fluid exchange and cell cytoarchitecture.\"","abstract_html":""Finally, studies were designed to help define the physiological role of estrogen-induction of the NHE-RF gene using two model systems. Immunocytochemistry was used to visualize NHE-RF in MCF-7 cells. Secondly, we created adenovirus that express the wild type or anti-sense NHE-RF RNA. By infecting cells with the adenovirus we may overexpress or functionally ""knock out"" the NHE-RF protein and monitor the resulting effects on cell signaling, fluid exchange and cell cytoarchitecture."","abstract_has_math":false,"creators":["Ediger, Tracy Rene"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Katzenellenbogen, Benita S."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-28T15:17:07Z","date_published":"2015-09-28T15:17:07Z","updated_at":"2026-07-22T22:26:27Z","subjects":["Biology, Animal Physiology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3070296"],"render_values":[{"text":"(MiAaPQ)AAI3070296","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/86642","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Katzenellenbogen, Benita S."]},{"key":"dc:creator","label":"Author","values":["Ediger, Tracy Rene"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-28T15:17:07Z","10000-01-01","2002"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Animal Physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/86642","(MiAaPQ)AAI3070296"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["\"Finally, studies were designed to help define the physiological role of estrogen-induction of the NHE-RF gene using two model systems. 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