{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/86303"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/86303","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Imaging Neuromuscular Synaptogenesis","abstract":"Successful synaptogenesis requires collaboration of cellular and molecular dynamics. Whereas synaptic matchmaking relies on cell-specific recognition molecules, subsequent synaptogenesis requires proteins that are common to a given synaptic type. Biochemical studies reveal that PSD-95/Dlg family scaffolding proteins could coordinate postsynaptic molecular assembly at glutamatergic synapses. Genetic studies demonstrate their role during synaptic maintenance and plasticity. However, it is unclear whether initial synaptogenesis requires PSD-95/Dlg and, if so, how its function collaborates with cell-specific matchmaking that precedes synaptogenesis. Here, using whole-mount high-resolution live imaging, we show that glutamatergic synaptogenesis on all muscles in Drosophila embryos is preceded by formation of a myopodial cluster into which PSD-95/Dlg is rapidly recruited. Cell-specific disruption of myopodial clustering affect synaptogenesis, while disrupting postsynaptic PSD-95/Dlg functions during this period aborts synaptogenesis without affecting myopodial cluster formation. These results support essential functions of postsynaptic PSD-95/Dlg and its collaboration with myopodial cluster at the onset of glutamatergic neuromuscular synaptogenesis.","abstract_html":"Successful synaptogenesis requires collaboration of cellular and molecular dynamics. Whereas synaptic matchmaking relies on cell-specific recognition molecules, subsequent synaptogenesis requires proteins that are common to a given synaptic type. Biochemical studies reveal that PSD-95/Dlg family scaffolding proteins could coordinate postsynaptic molecular assembly at glutamatergic synapses. Genetic studies demonstrate their role during synaptic maintenance and plasticity. However, it is unclear whether initial synaptogenesis requires PSD-95/Dlg and, if so, how its function collaborates with cell-specific matchmaking that precedes synaptogenesis. Here, using whole-mount high-resolution live imaging, we show that glutamatergic synaptogenesis on all muscles in Drosophila embryos is preceded by formation of a myopodial cluster into which PSD-95/Dlg is rapidly recruited. Cell-specific disruption of myopodial clustering affect synaptogenesis, while disrupting postsynaptic PSD-95/Dlg functions during this period aborts synaptogenesis without affecting myopodial cluster formation. These results support essential functions of postsynaptic PSD-95/Dlg and its collaboration with myopodial cluster at the onset of glutamatergic neuromuscular synaptogenesis.","abstract_has_math":false,"creators":["Carrero-Martinez, Franklin Alexander"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Cell and Developmental Biology","degree_department":null,"school":null,"contributors":["Chiba, Akira"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-28T15:03:15Z","date_published":"2015-09-28T15:03:15Z","updated_at":"2026-07-22T22:26:27Z","subjects":["Biology, Cell"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3202069"],"render_values":[{"text":"(MiAaPQ)AAI3202069","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/86303","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Chiba, Akira"]},{"key":"dc:creator","label":"Author","values":["Carrero-Martinez, Franklin Alexander"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-28T15:03:15Z","10000-01-01","2005"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Cell and Developmental Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Cell"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/86303","(MiAaPQ)AAI3202069"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Successful synaptogenesis requires collaboration of cellular and molecular dynamics. 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Cell-specific disruption of myopodial clustering affect synaptogenesis, while disrupting postsynaptic PSD-95/Dlg functions during this period aborts synaptogenesis without affecting myopodial cluster formation. These results support essential functions of postsynaptic PSD-95/Dlg and its collaboration with myopodial cluster at the onset of glutamatergic neuromuscular synaptogenesis.","Made available in DSpace on 2015-09-28T15:03:15Z (GMT). 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Whereas synaptic matchmaking relies on cell-specific recognition molecules, subsequent synaptogenesis requires proteins that are common to a given synaptic type. Biochemical studies reveal that PSD-95/Dlg family scaffolding proteins could coordinate postsynaptic molecular assembly at glutamatergic synapses. Genetic studies demonstrate their role during synaptic maintenance and plasticity. However, it is unclear whether initial synaptogenesis requires PSD-95/Dlg and, if so, how its function collaborates with cell-specific matchmaking that precedes synaptogenesis. Here, using whole-mount high-resolution live imaging, we show that glutamatergic synaptogenesis on all muscles in Drosophila embryos is preceded by formation of a myopodial cluster into which PSD-95/Dlg is rapidly recruited. Cell-specific disruption of myopodial clustering affect synaptogenesis, while disrupting postsynaptic PSD-95/Dlg functions during this period aborts synaptogenesis without affecting myopodial cluster formation. These results support essential functions of postsynaptic PSD-95/Dlg and its collaboration with myopodial cluster at the onset of glutamatergic neuromuscular synaptogenesis.","Made available in DSpace on 2015-09-28T15:03:15Z (GMT). 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