{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/85475"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/85475","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Molecular Mechanisms of Gating, Selectivity, and Transport in Membrane Channels and Carriers","abstract":"Exchange of materials and information across biological membranes is one of the most fundamental and highly regulated processes in the biology of all living organisms. As lipid bilayers present a significant barrier to the transport of most materials, specialized membrane channels and carriers have been evolved to facilitate their transport across biological membranes. The water channel aquaporin (AQP) and the nucleotide transporter ADP/ATP carrier (AAC) are the focus of the research presented in this thesis. By facilitating water conduction across cellular membranes, AQPs play a significant role in water homeostasis in living cells. Of similar importance, AAC mediates the exchange of ADP and ATP across the mitochondrial membrane and is fundamental to energy production in eukaryotic cells. Using molecular dynamics simulations, the substrate selectivity, gas permeability and gating mechanism of AQPs, as well as the substrate binding and induced translocation in AAC, are investigated. Two AQPs from the same organism with different functionalities are used to identify key structural features determining the substrate selectivity of AQPs. Multiple sampling methodologies are applied to investigate the permeability of mammalian AQP1 and AQP4 to O2, CO2 and NO. Apart from water, these AQPs are found to provide pathways for gas molecules to cross biological membranes. Additionally, the phosphorylation-mediated gating of a plant aquaporin is investigated. Opening of the water channel upon phosphorylation is shown to result from coupled motion of a conserved cytoplasmic loop and a hydrophobic residue physically blocking the channel. In the last part of the thesis, insights into the nucleotide binding and translocation process mediated by AAC are presented. A rapid, spontaneous binding of ADP to AAC is revealed by multiple simulations, which is found to be driven by a strong electrostatic potential produced by the positively charged protein. The commonality and significance of this electrostatic potential in other mitochondrial carriers is also discussed.","abstract_html":"Exchange of materials and information across biological membranes is one of the most fundamental and highly regulated processes in the biology of all living organisms. As lipid bilayers present a significant barrier to the transport of most materials, specialized membrane channels and carriers have been evolved to facilitate their transport across biological membranes. The water channel aquaporin (AQP) and the nucleotide transporter ADP/ATP carrier (AAC) are the focus of the research presented in this thesis. By facilitating water conduction across cellular membranes, AQPs play a significant role in water homeostasis in living cells. Of similar importance, AAC mediates the exchange of ADP and ATP across the mitochondrial membrane and is fundamental to energy production in eukaryotic cells. Using molecular dynamics simulations, the substrate selectivity, gas permeability and gating mechanism of AQPs, as well as the substrate binding and induced translocation in AAC, are investigated. Two AQPs from the same organism with different functionalities are used to identify key structural features determining the substrate selectivity of AQPs. Multiple sampling methodologies are applied to investigate the permeability of mammalian AQP1 and AQP4 to O2, CO2 and NO. Apart from water, these AQPs are found to provide pathways for gas molecules to cross biological membranes. Additionally, the phosphorylation-mediated gating of a plant aquaporin is investigated. Opening of the water channel upon phosphorylation is shown to result from coupled motion of a conserved cytoplasmic loop and a hydrophobic residue physically blocking the channel. In the last part of the thesis, insights into the nucleotide binding and translocation process mediated by AAC are presented. A rapid, spontaneous binding of ADP to AAC is revealed by multiple simulations, which is found to be driven by a strong electrostatic potential produced by the positively charged protein. The commonality and significance of this electrostatic potential in other mitochondrial carriers is also discussed.","abstract_has_math":false,"creators":["Wang, Yi"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biophysics and Computational Biology","degree_department":null,"school":null,"contributors":["Tajkhorshid, Emad","Schulten, Klaus J."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:46:12Z","date_published":"2015-09-25T22:46:12Z","updated_at":"2026-07-22T22:26:25Z","subjects":["Biophysics, General"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3347559"],"render_values":[{"text":"(MiAaPQ)AAI3347559","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/85475","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Tajkhorshid, Emad","Schulten, Klaus J."]},{"key":"dc:creator","label":"Author","values":["Wang, Yi"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:46:12Z","10000-01-01","2008"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biophysics and Computational Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biophysics, General"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/85475","(MiAaPQ)AAI3347559"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Exchange of materials and information across biological membranes is one of the most fundamental and highly regulated processes in the biology of all living organisms. As lipid bilayers present a significant barrier to the transport of most materials, specialized membrane channels and carriers have been evolved to facilitate their transport across biological membranes. The water channel aquaporin (AQP) and the nucleotide transporter ADP/ATP carrier (AAC) are the focus of the research presented in this thesis. By facilitating water conduction across cellular membranes, AQPs play a significant role in water homeostasis in living cells. Of similar importance, AAC mediates the exchange of ADP and ATP across the mitochondrial membrane and is fundamental to energy production in eukaryotic cells. Using molecular dynamics simulations, the substrate selectivity, gas permeability and gating mechanism of AQPs, as well as the substrate binding and induced translocation in AAC, are investigated. Two AQPs from the same organism with different functionalities are used to identify key structural features determining the substrate selectivity of AQPs. Multiple sampling methodologies are applied to investigate the permeability of mammalian AQP1 and AQP4 to O2, CO2 and NO. Apart from water, these AQPs are found to provide pathways for gas molecules to cross biological membranes. Additionally, the phosphorylation-mediated gating of a plant aquaporin is investigated. Opening of the water channel upon phosphorylation is shown to result from coupled motion of a conserved cytoplasmic loop and a hydrophobic residue physically blocking the channel. In the last part of the thesis, insights into the nucleotide binding and translocation process mediated by AAC are presented. A rapid, spontaneous binding of ADP to AAC is revealed by multiple simulations, which is found to be driven by a strong electrostatic potential produced by the positively charged protein. The commonality and significance of this electrostatic potential in other mitochondrial carriers is also discussed.","Made available in DSpace on 2015-09-25T22:46:12Z (GMT). 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As lipid bilayers present a significant barrier to the transport of most materials, specialized membrane channels and carriers have been evolved to facilitate their transport across biological membranes. The water channel aquaporin (AQP) and the nucleotide transporter ADP/ATP carrier (AAC) are the focus of the research presented in this thesis. By facilitating water conduction across cellular membranes, AQPs play a significant role in water homeostasis in living cells. Of similar importance, AAC mediates the exchange of ADP and ATP across the mitochondrial membrane and is fundamental to energy production in eukaryotic cells. Using molecular dynamics simulations, the substrate selectivity, gas permeability and gating mechanism of AQPs, as well as the substrate binding and induced translocation in AAC, are investigated. Two AQPs from the same organism with different functionalities are used to identify key structural features determining the substrate selectivity of AQPs. Multiple sampling methodologies are applied to investigate the permeability of mammalian AQP1 and AQP4 to O2, CO2 and NO. Apart from water, these AQPs are found to provide pathways for gas molecules to cross biological membranes. Additionally, the phosphorylation-mediated gating of a plant aquaporin is investigated. Opening of the water channel upon phosphorylation is shown to result from coupled motion of a conserved cytoplasmic loop and a hydrophobic residue physically blocking the channel. In the last part of the thesis, insights into the nucleotide binding and translocation process mediated by AAC are presented. A rapid, spontaneous binding of ADP to AAC is revealed by multiple simulations, which is found to be driven by a strong electrostatic potential produced by the positively charged protein. The commonality and significance of this electrostatic potential in other mitochondrial carriers is also discussed.","Made available in DSpace on 2015-09-25T22:46:12Z (GMT). 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