{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/85455"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/85455","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Structural and Biochemical Studies of Nisin *Biosynthesis and Autoimmunity","abstract":"The lantibiotic nisin is a 34-residue bacteriocin produced by the Gram-positive bacteria Lactococcus lactis spp. It is first ribosomally synthesized as an inactive precursor which is then post-translationally modified into its mature biologically active form. Described herein is a two-part study investigating (1) the structural basis of how the characteristic nisin lanthionine rings are installed by the enzyme NisC and (2) how Lactococcus lactis spp., despite producing an effective bacteriocin like nisin, is resistant to the toxic effects of nisin. The results described here together provide the first structural insights into the biosynthesis of nisin as well as insights into how L. lactis demonstrates autoimmunity to the lantibiotic nisin.","abstract_html":"The lantibiotic nisin is a 34-residue bacteriocin produced by the Gram-positive bacteria Lactococcus lactis spp. It is first ribosomally synthesized as an inactive precursor which is then post-translationally modified into its mature biologically active form. Described herein is a two-part study investigating (1) the structural basis of how the characteristic nisin lanthionine rings are installed by the enzyme NisC and (2) how Lactococcus lactis spp., despite producing an effective bacteriocin like nisin, is resistant to the toxic effects of nisin. The results described here together provide the first structural insights into the biosynthesis of nisin as well as insights into how L. lactis demonstrates autoimmunity to the lantibiotic nisin.","abstract_has_math":false,"creators":["Yu, John-Paul Jaewoon"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biophysics and Computational Biology","degree_department":null,"school":null,"contributors":["Nair, Satish K."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:46:09Z","date_published":"2015-09-25T22:46:09Z","updated_at":"2026-07-22T22:26:25Z","subjects":["Biophysics, General"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3223758"],"render_values":[{"text":"(MiAaPQ)AAI3223758","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/85455","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Nair, Satish K."]},{"key":"dc:creator","label":"Author","values":["Yu, John-Paul Jaewoon"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:46:09Z","10000-01-01","2006"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biophysics and Computational Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biophysics, General"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/85455","(MiAaPQ)AAI3223758"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The lantibiotic nisin is a 34-residue bacteriocin produced by the Gram-positive bacteria Lactococcus lactis spp. It is first ribosomally synthesized as an inactive precursor which is then post-translationally modified into its mature biologically active form. 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